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Normal-temperature and low-temperature boar sperm diluent based on chlorpromazine

A technology of chlorpromazine and diluent, applied in the field of sperm diluent, can solve the problems of high sperm energy consumption, affecting the vitality and survival rate of pig sperm, and achieve the effect of good integrity

Inactive Publication Date: 2019-05-17
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the in vitro storage of pig sperm is usually stored at 16.5-17°C. Once the temperature is lower than this temperature, the vitality and survival rate of pig sperm will be seriously affected.
Pig sperm is very sensitive to temperature changes, especially cooling, but high temperature will lead to high energy consumption of sperm, so it is necessary to study the dilution of pig sperm cryopreservation

Method used

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  • Normal-temperature and low-temperature boar sperm diluent based on chlorpromazine
  • Normal-temperature and low-temperature boar sperm diluent based on chlorpromazine
  • Normal-temperature and low-temperature boar sperm diluent based on chlorpromazine

Examples

Experimental program
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Effect test

Embodiment 1

[0021] A kind of normal temperature dilution of pig sperm based on chlorpromazine provided by the present embodiment, said normal temperature dilution contains chlorpromazine-based cell dormancy system, glucose 11g, fructose 17.5g, lemon sodium bicarbonate 1.0g, EDTA-2Na 2.35g, sodium bicarbonate 1.0g, Tris buffer 5.65g, citric acid 2.9g, penicillin 0.1-0.3g, streptomycin 0.1-0.3g; Dissolve in aqueous solution after stirring, filter and sterilize, and freeze for later use; when used, it can be isothermally diluted with sperm according to a certain volume ratio.

[0022] The cell dormancy system based on the chlorpromazine is chlorpromazine 1-4mg / mL, meperidine 1-4mg / mL, promethazine 1-4mg / mL, phenobarbital 10-100 μg / mL, hydrogenated Ergot alkaloid 10-50μg / mL.

[0023] The method of using the diluent is to prepare the diluent and pass it through a 0.22 micron filter to sterilize and store it in a freezer. The temperature is kept constant while the diluent and sperm are mixed....

Embodiment 2

[0026] A kind of pig sperm normal temperature diluent based on chlorpromazine provided by the present embodiment, its difference with embodiment 1 is that the cell dormancy system based on said chlorpromazine is chlorpromazine 1-4mg / mL, iso Procaine 1-4mg / mL, acepromazine 0.2-1.2mg / mL, spadine 100-200μg / mL, procaine 10-40mg / mL.

Embodiment 3

[0028] A kind of low-temperature diluent of porcine sperm based on chlorpromazine provided by the present embodiment, said low-temperature diluent contains the cell dormancy system based on chlorpromazine in every 1 L of diluent, and the yolk liquid of soft-shelled turtle for later use after centrifugation 20mL, glucose 30.0g, sucrose 40.0g, sodium citrate 4.0g, penicillin 0.1-0.3g, streptomycin 0.1-0.3g, DMSO (dimethyl sulfoxide) 10-40g; Dissolve in aqueous solution after stirring, filter and sterilize, and freeze for later use; when used, it can be isothermally diluted with sperm according to a certain volume ratio.

[0029] The preparation method of the standby Chinese soft-shelled turtle yolk liquid after the centrifugation is to freeze the Chinese soft-shelled turtle eggs under low temperature conditions, cut the shell to get the yolk after comprehensive disinfection; the yolk and distilled water are prepared in proportion to a 10% yolk solution at room temperature, After...

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Abstract

The invention discloses a normal-temperature boar sperm diluent based on chlorpromazine and a low-temperature boar sperm diluent based on chlorpromazine and Chinese softshell turtle yolk. The in-vitrostorage temperature of boar sperm can be decreased to 10 DE C, preferably 12 DEG C by the normal-temperature diluent. The temperature of sperm stored at low temperature can be decreased to 4 DEG C, preferably 6 DEG C. Histological analysis proves that the integrity of acrosome of sperm stored at reduced temperature is obviously better than that of acrosome stored at normal temperature.

Description

technical field [0001] The invention relates to a sperm diluent, in particular to a normal temperature and low temperature diluent of pig sperm based on chlorpromazine. Background technique [0002] With the continuous intensification, specialization, ecologicalization and technological development of my country's pig industry, the artificial insemination technology of pigs has received more and more attention in the modern pig industry. However, the in vitro storage of pig sperm is usually stored at 16.5-17°C. Once the temperature is lower than this temperature, the vitality and survival rate of pig sperm will be seriously affected. Pig sperm is very sensitive to temperature changes, especially cooling, but higher temperature will lead to high energy consumption of sperm, so it is necessary to study the dilution of pig sperm for cryopreservation. Contents of the invention [0003] The purpose of the present invention is to provide a kind of normal temperature and low tem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 陈忠法王伟钱国英尹尚军
Owner ZHEJIANG WANLI UNIV
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