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Method for evaluating toxicity of Microcystin in real water environment

A technology for microcystins and water environment, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, and material testing products, etc. It can solve the problem that the potential toxicity hazards of microcystins are not reflected, and the research results are not convincing. and other issues, to achieve universal applicability, wide application range and good application prospects.

Active Publication Date: 2019-05-21
HEFEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] These devices and methods only detect and analyze the content of microcystins, and do not reflect the potential toxicity hazards of microcystins; and the current methods for evaluating the toxicity of microcystins are all based on a single microcystin solution. Yes, if the application of the research results in environmental water microcystin toxicity evaluation is considered, the convincing results will be less convincing because the real water environment is not restored

Method used

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  • Method for evaluating toxicity of Microcystin in real water environment
  • Method for evaluating toxicity of Microcystin in real water environment
  • Method for evaluating toxicity of Microcystin in real water environment

Examples

Experimental program
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Effect test

Embodiment 1

[0048] This embodiment includes the following steps:

[0049] (1) The degraded strain M9 before irradiation was prepared into a bacterial cell suspension and diluted to 10 9 pcs / mL; prepare the stock solution for coexistence of degrading strains and MC-LR;

[0050] The preparation method of the stock solution in which the degrading strain and MC-LR coexist is: take 200 μL of 50 μM pure MC-LR stock solution and add it to the bacterial cell concentration of 10 9 cells / mL in 800 μL cell suspension, the volume ratio of cell suspension to MC-LR solution in the prepared solution is 4:1; shake evenly, and incubate for three days at room temperature in the dark; then use a 0.22 μm pore size filter membrane Filtered and stored in a -20°C refrigerator to obtain a stock solution in which the degraded strains and MC-LR coexist.

[0051] (2) MC-LR and strain coexisting working solution with concentrations of 1 μM and 0.1 μM, pure MC-LR working solution of 1 μM and 0.1 μM, and degraded st...

Embodiment 2

[0062] The degrading strains irradiated by ion beams were prepared with MC-LR to prepare a coexisting working solution to infect Hep G2 cells and carry out MTT experiments, and then the absorbance value was detected. The strength of the degradation effect.

[0063] This embodiment includes the following steps:

[0064] (1) through N + The degrading strain M9 was irradiated by ion beam implantation, and the degrading strains A1, A2 and A3 were obtained after irradiation;

[0065] A1: The radiation dose is 40 units, each unit is 2.6×10 13 N + / cm 2 ,

[0066] A2: The radiation dose is 60 units, each unit is 2.6×10 13 N + / cm 2 ,

[0067] A3: The radiation dose is 80 units, each unit is 2.6×10 13 N + / cm 2 ,

[0068] (2) The degrading strain M9 before irradiation and the degrading strains A1, A2 and A3 after irradiation were prepared into bacterial cell suspension, and diluted to 10 9 pcs / mL; then prepare the stock solutions of the coexistence of degrading strains M...

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Abstract

The invention discloses a method for evaluating toxicity of Microcystin in a real water environment. The method comprises the following steps: preparing coexistence liquid of degrading strains and Microcystin-LR (MC-LR) and constructing a contamination sample with the form close to that of the MC-LR in a real water environment; then infecting human hepatoma carcinoma cells, and verifying toxicityof MC-LR under the condition of coexistence of the degrading strains by using an MTT method for detecting cell metabolic activity. According to the invention, the state of MC-LR in coexistence with the degrading strain in real water environments such as lakes is simulated, and the Limitation of toxicity research on single MC-LR is made up for, the toxicity of MC-LR is better evaluated, and the application prospect in the industries of aquaculture, environmental governance and the like is good. The method has universal applicability to various Microcystin isomers and is wide in application range. According to the method, the degrading strains is irradiated by an N+ ion beam injection technology to obtain irradiated degrading strains, the irradiated degrading strains are compared with the degrading strains before irradiation in the aspect of degrading effect on MC-LR, and then the feasibility of the method is verified.

Description

technical field [0001] The invention relates to a method for evaluating the toxicity of microcystins, in particular to a method for evaluating the toxicity of microcystins in a real water environment. Background technique [0002] Microcystins are the most widely distributed class of liver toxins, mainly produced by the freshwater alga Microcystis aeruginosa, with high stability. It can strongly inhibit the activity of protein phosphatase and is also a strong hepatic tumor promoter. The Chinese drinking water standard limits the content of this toxin in drinking water to 1 μg / L. [0003] Since the 1950s, with the development of social science, technology and culture, due to the abuse of phosphorus-containing washing powder, pesticides, etc., water body eutrophication, and water body eutrophication can promote the growth of cyanobacteria and other algae plants, so that the water surface forms a Thick layers of blue-green lake indigo, unpleasant taste. Among the various alg...

Claims

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Application Information

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IPC IPC(8): G01N33/50C12Q1/02
Inventor 王晓飞肖厚荣张敏吴茜茜鲍腾
Owner HEFEI UNIV
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