Soil and water loss control method based on microbial mineralization

A technology of mineralization and microorganisms, applied in the direction of chemical instruments and methods, applications, soil protection, etc., can solve the problems of low curing reaction efficiency, unrepresentative, MICP technology is not suitable for strengthening cohesive soil, etc., to achieve improvement Infiltration efficiency and infiltration depth, good air permeability and water permeability, good infiltration and diffusion performance

Inactive Publication Date: 2019-05-24
NANJING UNIVERSTIY SUZHOU HIGH TECH INST
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Problems solved by technology

[0006] "Journal of Agricultural Engineering" in June 2017, Volume 33, Issue 11, pages 133-139, published "Analysis of Factors Influencing the Strength and Erosion Resistance of Silt Slopes Protected by Microbial Mortar", which discloses the use of microbial mortar layers to protect silt surfaces. The method of protective treatment includes the step of sandblasting the surface of the silt, and the steps of repeatedly spraying the microbial solution and the cementing solution alternately, which can protect the silt slope to a certain extent, but it still exists. Certain limitations: (1) this prior art carries out sandblasting treatment to the silt surface at first, then sprays the microbial bacterium liquid on the sand, then sprays the cementing solution, and its purpose is to reinforce the sand layer to form a microbial mortar protective layer, and It is not a direct reinforcement of the soil. Since the sand contains large pores and has similar properties to calcium carbonate, it is easier to use microbial reinforcement to consolidate the sand than the direct reinforcement of silt. However, this method is directly used for silt or silt. It is difficult to achieve the effect when the general soil is reinforced; (2) in the actual application of soil solidification on a large area slope, the steps of sandblasting are complicated and do not meet the actual working conditions; (3) the culture medium uses tryptone, soybean The mixed solution of peptone, sodium chloride and urea has complex components, which leads to high project cost; (4) only the mixed solution of urea and calcium chloride is used as the cementing solution, the cementing effect is not ideal and the curing reaction efficiency is low, which cannot meet the requirements actual working conditions
However, it must be pointed out that soil types in nature vary greatly. The sandy soil composed of pure quartz sand within a specific gradation range in the existing research is only an ideal special case, which is artificially prepared to meet specific requirements. Kind of sandy soil is not common in nature and not representative
The soils where water and soil loss occurs in nature are basically cohesive soils with more fine particles. Because the pore size in cohesive soils is relatively small, it is generally considered that MICP technology is not suitable for strengthening cohesive soils. Therefore, the current academic circles There are relatively few research reports on the prevention and control of soil and water loss by microbial mineralization

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  • Soil and water loss control method based on microbial mineralization
  • Soil and water loss control method based on microbial mineralization
  • Soil and water loss control method based on microbial mineralization

Examples

Experimental program
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Effect test

Embodiment 1

[0052] The soil in this example is a horizontal soil with a permeability coefficient of 5×10 -4 m / s.

[0053] The microbial bacterial liquid adopts Bacillus sarcina pasteuri, and the medium is: yeast extract 20g / L, ammonium sulfate 10g / L, 0.13mol Tris-Buffer 15.75g / L; the bacterial liquid is activated when taking it, and the temperature during the activation process is controlled at 25°C, the shaker speed is 120rmp / min, and the pH is controlled at 8.1; the activated bacterial liquid is stored in a refrigerator at 4°C; the enzyme activity level of the microbial liquid is controlled by the conductivity method to be 1.8mS / (cm min -1 ), using a spectrophotometer to control the absorbance value OD of the bacterial solution concentration at a wavelength of 600nm 600 is 1.0Abs.

[0054] Concentration of cementing liquid is 0.1M, and each liter contains: 6.0g of urea, 11g of calcium chloride, and 3g of nutrient broth.

[0055] A method for preventing and controlling water and soil ...

Embodiment 2

[0061] The processing steps of this embodiment are basically the same as in Embodiment 1, the difference is that the permeability coefficient is selected to be 5×10 -5 The horizontal soil mass of m / s is tested, and the experimental results show that: figure 1 As shown, microorganisms form a layer of 2.5cm cemented and solidified layer on the soil surface. Compared with the untreated soil, the solidified layer increases the penetration resistance of the soil surface by 15%, and the soil erosion rate decreases by 10%, illustrating this embodiment. method for permeability coefficients less than 10 -4 The soil mass of m / s also has a certain curing effect, but the effect is not as good as the permeability coefficient greater than 10. -4 m / s of soil.

Embodiment 3

[0063] The soil in this embodiment is a 35° slope, and the soil permeability coefficient is 5×10 -4 m / s.

[0064] The microbial bacterial liquid adopts Bacillus sarcina pasteuri, and the medium is: yeast extract 20g / L, ammonium sulfate 10g / L, 0.13mol Tris-Buffer 15.75g / L; the bacterial liquid is activated when taking it, and the temperature during the activation process is controlled at 25°C, shaker speed is 120rmp / min; the activated bacterial liquid is stored in a refrigerator at 4°C; the enzyme activity level of the microbial liquid is controlled by the conductivity method to be 1.9mS / (cm min -1 ), using a spectrophotometer to control the absorbance value OD of the bacterial solution concentration at a wavelength of 600nm 600 It is 1.4Abs.

[0065] Concentration of cementing liquid is 0.5M, and each liter contains: 30g of urea, 55g of calcium chloride, and 3g of nutrient broth.

[0066] A method for preventing and controlling water and soil loss based on microbial mineral...

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Abstract

The invention discloses a soil and water loss control method based on microbial mineralization, and belongs to the field of soil and water conservation. The soil and water loss control method based onmicrobial mineralization comprises the following steps: (1) slowly spraying urease-producing microbial liquid on the surface of a soil body, and keeping for 6-12 h after spraying; (2) slowly sprayinga cementing solution on the surface of the soil body after the microbial liquid is sprayed, and keeping for 6-12 h; and (3) repeating the step (1) and the step (2) 3-5 times, and standing for air drying. According to the invention, the microbial liquid is directly and slowly sprayed on the surface of the soil body, the aim of directly carrying out microbial mineralization reinforcement on the soil body is achieved through an MICP technology, and the erosion resistance of the soil body is effectively improved. The method is simple in steps, can effectively control soil and water loss, and is asimple and efficient method for controlling soil and water loss.

Description

technical field [0001] The invention belongs to the field of soil and water conservation, and more specifically relates to a method for preventing and controlling soil and water loss based on microbial mineralization. Background technique [0002] my country is one of the countries with the most serious soil erosion in the world. Soil erosion has a wide range, great intensity, and serious harm, which are few and far between in the world. At present, the area of ​​soil erosion in my country is as high as 2.95 million km 2 , accounting for 30.7% of the country's land area, with an average annual soil loss of more than 5 billion tons. Severe soil erosion leads to the destruction of water and soil resources, the deterioration of the ecological environment, and the intensification of natural disasters, threatening the national ecological security, flood control safety, drinking water safety, food safety and normal production of various industries. It is of great scientific and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E02D3/12C09K17/40C09K105/00
Inventor 唐朝生谢约翰尹黎阳吕超刘博李昊蒋宁俊施斌程青
Owner NANJING UNIVERSTIY SUZHOU HIGH TECH INST
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