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HDL3 immune turbidimetry detection kit prepared based on polyclonal antibody and preparation and use method thereof

A polyclonal antibody and detection kit technology, which is applied in the field of immunological assay and analysis, can solve the problems of increased usage, impact, and time-consuming of R1 reagents

Active Publication Date: 2019-05-28
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The precipitation method is a method in which HDL3 is collected other than HDL3 by reagents containing divalent metal ions and dextran sulfate, and HDL3 in the supernatant part is recovered by centrifugation and measured with an automatic analysis device; it still has the following disadvantages: the operation requires skill , in addition, manual operations are required, and sometimes pre-processing operations for sample inspection are required, which takes too long and is not universal
However, this method has the following disadvantages: ① cannot identify subtypes of high-density lipoprotein cholesterol; ② easily affected by serum hemoglobin, bilirubin and triglycerides
Although it uses a surfactant that specifically recognizes HDL3-C to shield other lipoprotein components, which improves the specificity of identification, it cannot avoid the influence of high hemoglobin, bilirubin and triglyceride in serum, and Due to the shielding of HDL3-C, the influence of free cholesterol in the serum is more prominent, resulting in an increase in the use of R1 reagents, and the detection repeatability is not high

Method used

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  • HDL3 immune turbidimetry detection kit prepared based on polyclonal antibody and preparation and use method thereof
  • HDL3 immune turbidimetry detection kit prepared based on polyclonal antibody and preparation and use method thereof
  • HDL3 immune turbidimetry detection kit prepared based on polyclonal antibody and preparation and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] An HDL3 immunoturbidimetric assay kit prepared based on polyclonal antibodies in this embodiment includes two liquid components of reagent R1 and reagent R2 that are independent of each other, and the components and corresponding contents included are:

[0069] Reagent R1:

[0070]

[0071] The solvent is purified water;

[0072] Reagent R2:

[0073]

[0074] Its solvent is purified water.

[0075] In addition, HDL3 calibrator is also attached in the kit, and the components and corresponding contents included in the calibrator are as follows:

[0076]

[0077] Its solvent is purified water.

[0078] Further, the Polyoxyethylene styreneated phenyl ether derivative is specifically EMULGEN B-66, a product of Kao Corporation.

[0079] Further, the Polyoxyethylene polycyclic phenyl ether is specifically NIKKOL BC-15, a product of Nikko Chemical Company.

Embodiment 2

[0081] An HDL3 immunoturbidimetric assay kit prepared based on polyclonal antibodies in this embodiment includes two liquid components of reagent R1 and reagent R2 that are independent of each other, and the components and corresponding contents included are:

[0082] Reagent R1:

[0083]

[0084] The solvent is purified water;

[0085] Reagent R2:

[0086]

[0087] Its solvent is purified water.

[0088] In addition, HDL3 calibrator is also attached in the kit, and the components and corresponding contents included in the calibrator are as follows:

[0089]

[0090] Its solvent is purified water.

[0091] Further, the Polyoxyethylene styreneated phenyl ether derivative is specifically NIKKOL BC-10, a product of Nikko Chemical Company.

[0092] Further, the Polyoxyethylene polycyclic phenyl ether is specifically NIKKOL BD-10, a product of Nikko Chemical Company.

Embodiment 3

[0094] An HDL3 immunoturbidimetric assay kit prepared based on polyclonal antibodies in this embodiment includes two liquid components of reagent R1 and reagent R2 that are independent of each other, and the components and corresponding contents included are:

[0095] Reagent R1:

[0096]

[0097] The solvent is purified water;

[0098] Reagent R2:

[0099]

[0100] Its solvent is purified water.

[0101] In addition, HDL3 calibrator is also attached in the kit, and the components and corresponding contents included in the calibrator are as follows:

[0102]

[0103]

[0104] Its solvent is purified water.

[0105] Further, the Polyoxyethylene styreneated phenyl ether derivative is specifically a mixture of EMULGEN B-66 and EMULGEN A-90 from Kao Corporation and NIKKOL BC-10 from Nikko Chemicals.

[0106] Further, the Polyoxyethylene polycyclic phenyl ether is specifically a mixture of NIKKOL BC-15, NIKKOL BO-15V and NIKKOL BD-10 produced by Nikko Chemical Compa...

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PUM

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Abstract

The invention provides an HDL3 immuno turbidimetry detection kit prepared based on polyclonal antibody, which comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises Tris-HCl, PEG6000, BSA, NaCl, NaN3, EDTA, polyoxypyrene styrenylated phenyl ether derivative, and chicken anti-human HDL3 polyclonal antibody; and the reagent R2 comprises Tris-HCl, PEG6000, BSA, NaCl, NaN3, EDTA, polyoxypyrene polycylic phenyl ether. The invention also provides a preparation and use method of the kit. The kit uses the immune turbidimetry method to measure the HDL3, can directly measure the content of the HDL3, and has more reliable test result.

Description

technical field [0001] The invention belongs to the field of immunological determination and analysis, and relates to protein measurement and testing, in particular to a polyclonal antibody-based HDL3 immune turbidimetric detection kit and a preparation method thereof. Background technique [0002] High-density lipoprotein 3 (High-density lipoprotein 3, HDL3) is one of the subcomponents of high-density lipoprotein (High-density lipoprotein, HDL). HDL is one of the main lipoproteins in plasma, with the highest density and the smallest particle diameter. The protein composition is mainly apolipoprotein, and there are three subtypes of HDL1, HDL2 and HDL3. The particle size of human HDL is 7.2-12.9nm, the hydration density is between (1.063-1.210) g / mL, and the relative molecular mass is 200-400kD. The density of HDL3 is 1.125 g / mL-1.210 g / mL. [0003] HDL is mainly produced by the liver, and it is mainly broken down by the liver, and can also be synthesized in the small inte...

Claims

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Application Information

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IPC IPC(8): G01N33/92G01N33/53
Inventor 符修乐
Owner ANHUI DAQIAN BIO ENG LIMITED
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