A method for extracting fisetin in emblica

A technology of emblica and fisetin, applied in the direction of organic chemistry and the like, can solve the problems of long time, low purity of fisetin, low efficiency and the like, and achieve the effect of few separation steps and high separation purity

Active Publication Date: 2020-11-06
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing separation and purification processes mostly use multi-step solvent extraction methods. These methods have the disadvantage

Method used

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  • A method for extracting fisetin in emblica
  • A method for extracting fisetin in emblica
  • A method for extracting fisetin in emblica

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Embodiment 1, a kind of method of extracting fisetin in Emily

[0036] 1. Carry out secondary extraction with 70% ethanol aqueous solution, the material-liquid ratio of the first extraction is 1:8, the second extraction is 1:4, the extraction temperature is 65 ° C, and the extracts are combined and then concentrated and dried in vacuum;

[0037] 2. Pass the ethanol extract through a 100-mesh normal phase silica gel column, followed by petroleum ether:ethyl acetate (5:5), petroleum ether:ethyl acetate (1:9), ethyl acetate:methanol (5:5) , ethyl acetate: methanol (1:9), ethyl acetate: methanol: water (1:4:5) elution;

[0038] 3. Collect ethyl acetate:methanol (5:5) fractions, concentrate and dry, and then separate and purify by high performance liquid chromatography to obtain fisetin.

[0039] In order to qualitatively detect the obtained fisetin, HPLC-Q-TOF / MS detection technology was used for identification. HPLC-Q-TOF / MS detection conditions are as follows:

[0040]...

Example Embodiment

[0047] Embodiment 2, a kind of method for extracting fisetin in Emily, specifically comprises the steps:

[0048] 1. Carry out secondary extraction with 70% ethanol aqueous solution, the material-liquid ratio of the first extraction is 1:10, the second extraction is 1:4, the extraction temperature is 65°C, and the extracts are combined and then concentrated and dried in vacuum;

[0049] 2. Pass the ethanol extract through a 100-mesh normal phase silica gel column, followed by petroleum ether:ethyl acetate (5:5), petroleum ether:ethyl acetate (1:9), ethyl acetate:methanol (5:5) , ethyl acetate: methanol (1:9), ethyl acetate: methanol: water (1:4:5) elution;

[0050] 3. Collect ethyl acetate:methanol (5:5) fractions, concentrate and dry, and then separate and purify by high performance liquid chromatography to obtain fisetin. The chromatographic column is Diamonsil C18 (250mm×4.6mm, 5um); the column temperature: 30°C; the mobile phase is 0.2% formic acid aqueous solution-methan...

Example Embodiment

[0051] Embodiment 3, a kind of method for extracting fisetin in Emily, specifically comprises the steps:

[0052] 1. Carry out secondary extraction with 70% ethanol aqueous solution, the material-liquid ratio of the first extraction is 1:8, the second extraction is 1:6, the extraction temperature is 55 ° C, and the extracts are combined and then concentrated and dried in vacuum;

[0053] 2. Pass the ethanol extract through a 100-mesh normal phase silica gel column, followed by petroleum ether:ethyl acetate (5:5), petroleum ether:ethyl acetate (1:9), ethyl acetate:methanol (5:5) , ethyl acetate: methanol (1:9), ethyl acetate: methanol: water (1:4:5) elution;

[0054] 3. Collect ethyl acetate:methanol (5:5) fractions, concentrate and dry, and then separate and purify by high performance liquid chromatography to obtain fisetin. The chromatographic column is Diamonsil C18 (250mm×4.6mm, 5um); the column temperature: 30°C; the mobile phase is 0.2% formic acid aqueous solution-metha...

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Abstract

The invention belongs to the technical field of plant extraction and discloses a method for extracting lactoflavin from phyllanthus emblica. According to the method for extracting the lactoflavin in the phyllanthus emblica, three methods of phyllanthus emblica extraction, normal phase silica gel column chromatography and a preparation liquid chromatograph are cooperatively used, so that the methodis a novel and effective separation and purification method, the anti-inflammatory active ingredient lactoflavin in the phyllanthus emblica is effectively separated and purified, separation steps arefew, the purity of the separated lactoflavin is high, and the separation purity of the lactoflavin can reach 95% or above. The lactoflavin is separated from phyllanthus emblica for the first time, and reference is provided for separation and purification of active ingredients in extracts of other natural products.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting fisetin from emblica. Background technique [0002] Inflammation is a series of complex physiological changes that the body undergoes when it is subjected to external stimuli. With the emergence of drug resistance and adverse reactions to synthetic anti-inflammatory drugs, traditional Chinese medicinal materials are favored for their abundant resources, few side effects, and good curative effects. The development of natural anti-inflammatory drugs has become a research hotspot. Phyllanthus emblica is a traditionally edible fruit, a plant of the genus Euphorbiaceae, mainly distributed in tropical and subtropical countries such as India, Indonesia, Malaysia, and Hainan, Fujian, and Guangdong in my country. , Guangxi, Yunnan and other places, which are used in traditional medicine systems, such as traditional Chinese medicine, Tibetan med...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40
Inventor 李伟朱华伟宁嘉玲曹庸陈运娇郭晓蕾
Owner INFINITUS (CHINA) CO LTD
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