Method of separation and extraction of D-lactic acid from sodium D-lactate fermentation liquid

A technology for lactic acid fermentation and fermentation broth, applied in the field of bioengineering, can solve the problems of low degree of automation in the separation process, low product quality and high energy consumption, and achieve the target of reducing nitrogen content and post-decolorization costs, reducing energy consumption and acidification. short time effect

Active Publication Date: 2015-10-14
SINOPEC YANGZI PETROCHEM +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The process is mature and easy to control, but there are disadvantages such as long process route, many unit operations, serious waste liquid pollution, high energy consumpti

Method used

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Examples

Experimental program
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Example Embodiment

[0027] Example 1

[0028] The D-lactic acid fermentation broth produced by Bacillus strains, in which the concentration of D-lactate sodium is 100g / L, no residual sugar, and the pH of the fermentation broth is 6.0. Take the D-sodium lactate fermentation broth after fermentation to separate the bacteria through a disc centrifuge; then perform microfiltration and ultrafiltration to separate macromolecular proteins and some pigments. The molecular weight cut-off selected by the ultrafiltration membrane is 1000; Decolorization treatment, decolorization select powder activated carbon for decolorization, the decolorization pH is neutral, the decolorization temperature is 60℃, and the decolorization time is 30min; the decolorized fermentation broth is sent to the ion exchange column for ion exchange, the fermentation broth is first passed through the cation exchange resin After the anion exchange resin; finally the ion-exchanged fermentation broth is sent to the evaporator for vacuum ev...

Example Embodiment

[0029] Example 2

[0030] The D-lactic acid fermentation broth produced by Bacillus strains has a concentration of 120g / L of sodium D-lactate, no residual sugar, and the pH of the fermentation broth is 5.8. Take the D-sodium lactate fermentation broth after fermentation and directly enter the microfiltration and ultrafiltration system to separate the macromolecular protein and part of the pigment. The molecular weight cut-off of the ultrafiltration membrane is 5000; the fermentation broth is decolorized, and the decolorization is performed by the nanofiltration membrane. , The molecular weight cut-off used by the nanofiltration membrane is 300; the decolorized fermentation broth is sent to the ion exchange column for ion exchange, the fermentation broth is first passed through the cation exchange resin and then the anion exchange resin; finally the ion exchanged fermentation broth is sent to the evaporation D-lactic acid with an optical purity of 99% and a content of 91% is obtai...

Example Embodiment

[0031] Example 3

[0032] The D-lactic acid fermentation broth produced by Bacillus strains has a concentration of 110 g / L of D-lactate sodium, no residual sugar, and pH 6.0 of the fermentation broth. Take the D-sodium lactate fermentation broth after fermentation and directly enter the microfiltration and ultrafiltration system to separate the macromolecular protein and part of the pigment. The molecular weight cut-off of the ultrafiltration membrane is 20,000; the fermentation broth is decolorized, and the decolorization is performed in an activated carbon column. Select granular activated carbon, decolorization pH 6.0, decolorization temperature 60℃, decolorization time 30min; send the decolorized fermentation broth to the ion exchange column for ion exchange, the fermentation broth is passed through the cation exchange resin and then the anion exchange resin; The fermented broth after ion exchange is sent to an evaporator for vacuum evaporation to obtain D-lactic acid with an...

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Abstract

The invention discloses a method of separation and extraction of D-lactic acid from sodium D-lactate fermentation liquid, belongs to the technical field of bio-engineering and particularly relates to the method of separation and extraction of D-lactic acid from the fermentation liquid. The method includes the steps of (1) performing solid-liquid separation to the sodium D-lactate fermentation liquid, removing proteins and pigments to obtain a clarified fermentation liquid; (2) decoloring the clarified fermentation liquid obtained in the step (1); (3) performing ion exchange to the fermentation liquid obtained in the step (2); and (4) concentrating the fermentation liquid after the ion exchange to obtain the D-lactic acid. In the method, only is normal-temperature acidification required so that the method is short in acidification time. The method is effectively reduced in the content of protein and a part of the pigments in the fermentation liquid and is free of generation of calcium sulfate which is low in additional value and is difficult to treat. The method is optimized in operation and is less in separation steps.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for separating and purifying D-lactic acid in fermentation broth. Background technique [0002] Lactic acid is one of the three major organic acids in the world, it can be widely used in food, medicine, chemical industry and agriculture and other fields. The special D-lactic acid can be widely used in the fields of medicine, chemical industry and agriculture. Polylactic acid synthesized with D-lactic acid as a monomer has become the most promising green environmental protection material in the 21st century due to its good biodegradability and other excellent use characteristics (such as transparency, thermoplasticity, product safety, etc.). In addition, herbicides such as "Puma" and "Weiba", which are made of high-purity D-lactic acid, are new types of low-toxicity pesticides, which can effectively kill weeds and insects and are economical and reliabl...

Claims

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Application Information

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IPC IPC(8): C07C59/08C07C51/42
Inventor 方晓江刘经伟马江锋陈韶辉杨爱武李晓强柏基业
Owner SINOPEC YANGZI PETROCHEM
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