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Method for biologically synthesizing sclareolide

A biosynthetic technology of sclareolactone, which is applied in the food field and can solve problems such as cumbersome steps, unfriendly environment, and low safety

Inactive Publication Date: 2019-05-31
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a method for biosynthesizing sclareolide that can solve the problems of unfriendly environment, cumbersome steps and low safety in the process of chemically synthesizing sclareolactone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1) Take the yeast culture medium and add 3% sclareol and 6% cryptococcus albus protoplast liquid to the yeast culture medium to obtain a mixture;

[0013] 2) Put the mixture in a glass container, and then shake the flask for 10 days at 25° C. and 140 r / min on a shaker to obtain sclareolide.

Embodiment 2

[0015] 1) Take a yeast culture medium with a pH value of 6.0 and add 5% sclareol, 1.5% Tween 80 and 4% Cryptococcus albicans protoplasts which have undergone ultraviolet mutagenesis for 3 minutes to the yeast culture medium mixture of body fluids;

[0016] 2) Put the mixture in a glass container, and then shake the bottle on a shaker at 23° C. and 160 r / min for 10 days to obtain sclareolide.

Embodiment 3

[0018] 1) Take a yeast culture medium with a pH value of 6.0, and add 8% sclareol, 3% Tween 80 and 8% Cryptococcus albicans protoplasts which have undergone ultraviolet mutagenesis for 4 minutes to the yeast culture medium mixture of body fluids;

[0019] 2) Put the mixture in a glass container, and then shake the flask for 10 days at 26° C. and 150 r / min to ferment to obtain sclareolide.

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Abstract

The invention relates to a method for biologically synthesizing sclareolide. The method comprises the steps of taking a yeast culture medium and separately adding sclareol and a light white cryptococcus protoplast fluid into the yeast culture medium to obtain a mixture, wherein the sclareol accounts for 3-8% of the total mass of the yeast culture medium, and the light white cryptococcus protoplastfluid accounts for 4-10% of the total mass of the yeast culture medium; placing the mixture in a glass vessel, and then shaking the vessel for fermentation for 10 days on a shaking table at the temperature of 23-28 DEG C and the speed of 140-180 r / min to obtain sclareolide. According to the method, a microorganism conversion method is utilized, and sclareol is used as a substrate and converted bylight white cryptococcus to prepare sclareolide, so that the problems that a synthesis technology process of sclareolide based on a chemical method is environmentally unfriendly, complicated in step,low in safety and the like are solved, and a new way is provided for synthesizing sclareolide.

Description

technical field [0001] The invention belongs to the field of food, and in particular relates to a method for biosynthesizing sclareolactone. Background technique [0002] Sclareolide is an important food additive belonging to ambergris-flavored spices and an important intermediate for the synthesis of ambrox. Sclareolide can increase and improve the sensory properties of food, and has been widely used in the food industry. It can also be widely used in industries such as tobacco and cosmetics. At the same time, sclareolactone is also an intermediate in the synthesis of some drugs. It can be used in the pharmaceutical industry to synthesize drugs with anticancer effects. Therefore, the synthesis of sclareolide has high value. At present, the domestic synthesis of sclareolide usually adopts a chemical synthesis method, which needs to be obtained by oxidizing sclareol with an oxidant such as potassium permanganate. In this process, the use of a large number of oxidants and c...

Claims

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Application Information

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IPC IPC(8): C12P17/04C12R1/645
Inventor 刘树兴徐晨
Owner SHAANXI UNIV OF SCI & TECH
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