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Isoliquiritigenin and application thereof to treatment of acute lung and liver and brain tissue damage

A technology of isoliquiritigenin and lung, which is applied in the field of drugs for organ damage, can solve problems such as unconfirmed evidence, and achieve the effect of improving brain injury, improving the quality of life, and delaying the progression and deterioration of the disease

Pending Publication Date: 2019-06-07
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the treatment of traumatic brain injury, organ function support technology has made great progress, but there is no confirmed drug that can quickly and effectively delay the progression of injury

Method used

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  • Isoliquiritigenin and application thereof to treatment of acute lung and liver and brain tissue damage
  • Isoliquiritigenin and application thereof to treatment of acute lung and liver and brain tissue damage
  • Isoliquiritigenin and application thereof to treatment of acute lung and liver and brain tissue damage

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0053] Drug Toxicity Testing

[0054] Tetramethylazolium salt colorimetric method (MTT) test detects the change of cell viability of the hepatic cell line (HepG2) under the incubation of different concentrations of ISO: HepG2 cells are seeded in a 96-well plate, every 5000 cells, at 37 ° C Incubate for 24 hours; then cells were incubated with equal volumes of 1.25, 2.5, 5, 10, 20 or 40 μM ISO or DMSO (dimethyl sulfoxide) at 37° C. for 24 hours before performing MTT assay.

[0055] The cell viability was detected by the tetramethylazozolate colorimetric method, and the effect of ISO on the hepatocytes (HepG2) was analyzed. The results are shown in Figure 1 (A). No obvious toxicity.

experiment example 2

[0057] Construction of macrophage model, detection of inflammatory factors and related pathways

[0058] Studies have found that macrophages are mainly involved in the inflammatory response in lung or liver tissue, so macrophages are selected as the in vitro research object at the cellular level.

[0059] Primary macrophage extraction:

[0060] For the extraction method of primary macrophages, please refer to the reference (J Nutr Biochem 24, 146-155, PMID: 22819547, DOI: 10.1016 / j.jnutbio.2012.03.012).

[0061] qRT-PCR technology:

[0062] According to the instructions of the Trizol reagent, use the M-MLV reverse transcription kit to synthesize cDNA, take 2 μL of the prepared cDNA reaction solution as a template (guarantee that the Ct value of the internal reference and the target gene is in the range of 10-40, and the Ct value is the fluorescence in each reaction tube The number of cycles experienced when the signal reaches the set threshold), and the reaction system was p...

experiment example 3

[0083] Establishment and intervention of sepsis-induced lung and liver injury animal models

[0084] Thirty clean-grade male C57BL / 6 mice, aged 10-12 weeks, weighing 23-25 ​​g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., and were treated with LPS (20 mg·kg -1 Tail vein injection)-induced sepsis-induced lung and liver injury model.

[0085] Experimental group:

[0086] Model group (10 animals), using the above-mentioned modeling method;

[0087] The administration group (10 rats) was given ISO (20 mg·kg) 15 minutes after LPS induction -1 tail vein injection);

[0088] The control group (10 rats) was given the same volume of normal saline through the tail vein.

[0089] Real-time fluorescent quantitative PCR (qRT-PCR) was used to detect the inflammatory factors tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP- 1) mRNA expression, Western blot method to detect the degradation level of Iκ...

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Abstract

The invention provides an application of isoliquiritigenin to medicines for treatment of lung and liver damage diseases caused by septicopyemia, and an application of the isoliquiritigenin to medicines for treatment of brain tissue injury diseases caused by wounds. The medicines are favorable for delaying of progress deterioration of state of an illness, the case-fatality rate and the illness anddisability rate are notably reduced, and the quality of life is improved. The medicines are high in treatment maneuverability and low in cost, and the diagnosis and treatment cost for patients and medical institutions can be notably reduced.

Description

technical field [0001] The invention relates to a medicine for treating organ damage caused by acute diseases, in particular to a medicine for treating acute lung and liver injury induced by sepsis and acute brain injury caused by trauma. Background technique [0002] Sepsis and traumatic brain injury are two of the most common diseases in acute and critical illness. Sepsis refers to the systemic inflammatory response syndrome (SIRS) caused by infection, which often leads to multiple organ failure and poses a huge threat to human life and health. The fatality rate of sepsis is still as high as 30%. ~70%. [0003] Traumatic brain injury (TBI) refers to changes in brain function or pathology caused by external forces. In recent years, with the development of society and the prevalence of vehicles, the incidence of traumatic brain injury has increased year by year. In the United States, 30% of traumatic deaths are caused by traumatic brain injury, and its fatality rate and i...

Claims

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Application Information

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IPC IPC(8): A61K31/12A61P1/16A61P11/00A61P25/00A61P29/00A61P43/00A61P31/04A61P17/02
Inventor 陈雄陈咨苗陈肖俊吴文俊张曼洪广亮邱贤克潘学威乐融融施玉娟谷雪梅沈飞霞
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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