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Application of hederin in preparation of drug for preventing vascular endothelial cell inflammatory injury

A technology of ivy saponins and endothelial cells, which can be used in antibacterial drugs, anti-inflammatory agents, drug combinations, etc., can solve the problems of no report on the protective effect of vascular endothelial cells on inflammatory damage.

Active Publication Date: 2019-06-07
GUIZHOU NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protective effect of these compounds on inflammatory injury of vascular endothelial cells has not been reported

Method used

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  • Application of hederin in preparation of drug for preventing vascular endothelial cell inflammatory injury
  • Application of hederin in preparation of drug for preventing vascular endothelial cell inflammatory injury
  • Application of hederin in preparation of drug for preventing vascular endothelial cell inflammatory injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Preparation of double sugar chain hedera saponin

[0076] 10 kg of dried roots of Dipsacus chuanxiong, crushed, extracted with 95% ethanol (25L×2), combined two extracts, concentrated under reduced pressure to obtain extract. The extract was dispersed in 5L of water, filtered, and the filtrate was pretreated on the AB-8 macroporous adsorption resin column, firstly eluted with water until the color of the eluent was lighter, then eluted with 80% ethanol, collected 80% ethanol to wash Deliquification, concentration under reduced pressure to obtain the total saponins of Dipsacus chuanxiong. The total saponins of Dipsacus sativa were applied to a normal-phase silica gel chromatography column, and the gradient elution of chloroform-methanol-water system was used to obtain pure compound 1. After ESI-MS and 13 C-NMR data compared with literature data, identified as Dipsacus saponin VI (Asperosaponin VI).

[0077] Asperosaponin VI: white amorphous powder, ESI-MS: m / z927.3 ([...

Embodiment 2

[0079] Preparation of double sugar chain hedera saponin

[0080] 5 kg of dried flower buds of Lonicerae pilosula, crushed, extracted with 95% ethanol (25L×2), combined two extracts, concentrated under reduced pressure to obtain extract. The extract was dispersed in 5L of water, filtered, and the filtrate was pretreated on the AB-8 macroporous adsorption resin column, and first eluted with water until the color of the eluent was lighter, and then eluted with 50% methanol for 2 column volumes, Then elute with 80% ethanol, collect the 80% ethanol eluate, and concentrate under reduced pressure to obtain the total saponins of C. argentina. Take 100 g of total saponins of Lonicera tomentosa, put it on a normal-phase silica gel chromatography column, and elute with a gradient of chloroform-methanol-water system to obtain pure compounds 1 and 2, which are analyzed by ESI-MS and 13 The C-NMR data were compared with the data in the literature, and they were identified as Lonicera ciner...

Embodiment 3

[0084] Preparation of double sugar chain hedera saponin

[0085] 5 kg of dried flower buds of Lonicera chrysanthemum, crushed, extracted with 95% ethanol (25L×2), combined two extracts, concentrated under reduced pressure to obtain extract. The extract was dispersed in 5L of water, filtered, the filtrate was pretreated on the AB-8 macroporous adsorption resin column, and firstly eluted with water until the color of the eluent was lighter, and then eluted with 40% methanol for 2 column volumes, Then elute with 80% ethanol, collect the 80% ethanol eluate, and concentrate under reduced pressure to obtain total saponins of flavonoids (TSF). Take 100 g of total saponins of Lonicera tomentosa, put it on a normal-phase silica gel chromatography column, and elute with the gradient of chloroform-methanol-water system to obtain pure compound 1-3, which is analyzed by ESI-MS and 13 Comparing C-NMR data with literature data, they were identified as Dipsacoside B, Decaisoside E and Fulvot...

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Abstract

The present invention discloses an application of a double-sugar chain hederin compound in preparation of a drug for preventing vascular endothelial cell inflammatory injury. The compound can alleviate the vascular endothelial cell inflammatory injury by directly inhibiting adsorption of inflammatory factors and adhesion molecules, and regulating a NF-kappa-B signaling pathway and other various pathways, and plays a protection function of the vascular endothelial cell inflammatory injury. The application of the double-sugar chain hederin in the preparation of the drug for preventing the vascular endothelial cell inflammatory injury s is first disclosed, and the double-sugar chain hederin compound is rich in raw material source and mature in preparation method, and has good value in development of drugs for treating vascular endothelial cell inflammatory injury related acute lung injury, kidney injury, pyohemia, atherosclerosis, vascular complications of diabetes, etc.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to the application of a class of double sugar chain hedera saponins in the preparation of anti-vascular endothelial cell inflammatory injury drugs. Background technique [0002] Vascular endothelial cells (VEC) are a selectively permeable barrier covering between the blood vessel wall and the blood flow. They can synthesize and secrete substances that regulate the coagulation-fibrinolytic system, factors that regulate vascular tension, and cytokines. It plays an important role in angiogenesis, wound healing and inflammatory response (Endemann DH, et al. Endothelial dysfunction. JAm Soc Nephrol. 2004, 15(8): 1983–92.), and is an important part of the first barrier against foreign invasion part. When it senses external stimuli, it synthesizes and releases a variety of factors to regulate and exert functions such as hemostasis, cell growth, vasoconstr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P11/00A61P13/12A61P31/04A61P3/10A61P9/10A61P9/14A61P29/00A61P17/02
Inventor 杨庆雄孙黔云郭靖刘巧洲张军
Owner GUIZHOU NORMAL UNIVERSITY
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