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A compound preparation of egg yolk antibody against Chinese bee cystic larvae disease virus and its preparation

A technology of cystic larvae and Chinese honeybees, applied in the direction of antiviral agents, antiviral immunoglobulins, antibodies, etc., to achieve the effect of improving palatability, improving growth and development speed, disease resistance, and strong specificity

Active Publication Date: 2021-03-23
洛阳蜂藻康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the existence of such or such deficiencies in the above-mentioned multiple methods, the above-mentioned methods are limited in practical application.

Method used

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  • A compound preparation of egg yolk antibody against Chinese bee cystic larvae disease virus and its preparation
  • A compound preparation of egg yolk antibody against Chinese bee cystic larvae disease virus and its preparation
  • A compound preparation of egg yolk antibody against Chinese bee cystic larvae disease virus and its preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Artificial propagation and purification of CSBV

[0058] ①Artificial propagation of CSBV: Infect honeybee larvae with five strains of Chinese bee cystic larval disease virus strains from Jiangxi strain, Beijing strain, Shaanxi strain, Liaoning strain and Fujian strain, respectively, and mix the infected larvae; then take 5g to infect Chinese bee Honeybee larvae of honeybee cystic larvae disease virus were homogenized with a glass homogenizer until completely homogenized, centrifuged, and then the supernatant containing the virus was collected; the supernatant containing the virus was used to infect a batch of healthy bee larvae (80 2-day-old larvae), collect diseased larvae;

[0059] ② Purification of CSBV: Homogenize the honeybee larvae infected with the cystic larvae virus of Apis mellifera collected in step ① on an ice bath for 30 minutes until completely homogenized, centrifuge, and then collect the supernatant containing the virus; buffer with 0.2M PBS Resuspe...

Embodiment 2

[0068] (1) Artificial propagation and purification of CSBV

[0069] ①Artificial propagation of CSBV: Infect honeybee larvae with five strains of Chinese bee cystic larvae virus strains from Jiangxi strain, Beijing strain, Shaanxi strain, Liaoning strain and Fujian strain, respectively, and mix the infected larvae; then take 10g to infect Chinese bee Honeybee larvae of honeybee cystic larvae disease virus were homogenized to complete homogenization with a glass homogenizer, centrifuged, and then collected the supernatant containing the virus; the supernatant containing the virus was deinfected in 3 batches (240 larvae 2 days instar larvae) healthy bee larvae, collect diseased larvae;

[0070] ② Purification of CSBV: Homogenize the honeybee larvae infected with the cystic larvae virus of Apis mellifera collected in step ① on an ice bath for 5 minutes until completely homogenized, centrifuge, and then collect the supernatant containing the virus; buffer with 0.2M PBS Resuspend t...

Embodiment 3

[0079] (1) Artificial propagation and purification of CSBV

[0080] ①Artificial propagation of CSBV: Infect honeybee larvae with five strains of Chinese bee cystic larval disease virus strains from Jiangxi strain, Beijing strain, Shaanxi strain, Liaoning strain and Fujian strain respectively, and mix the infected larvae; then take 15g to infect Chinese bee Honeybee larvae of honeybee cystic larvae disease virus were homogenized to complete homogenization with a glass homogenizer, centrifuged, and then collected the supernatant containing the virus; the supernatant containing the virus was deinfected in 6 batches (480 larvae) 2 days instar larvae) healthy bee larvae, collect diseased larvae;

[0081] ② Purification of CSBV: Homogenize the honeybee larvae infected with the cystic larvae virus of Apis mellifera collected in step ① on an ice bath for 20 minutes until completely homogenized, centrifuge, and then collect the supernatant containing the virus; buffer with 0.2M PBS Re...

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Abstract

The invention belongs to the technical field of animal biological products, and specifically relates to a yolk antibody compound preparation capable of resisting Chinese Sacbrood Bee Virus and a preparation method thereof. The preparation method of the yolk antibody compound preparation capable of resisting the Chinese Sacbrood Bee Virus comprises the following steps: mixing a graphene oxide suspension with a chitosan solution, and carrying out stirring so as to obtain a graphene-oxide / chitosan adjuvant; adding a Chinese Sacbrood Bee Virus particle solution into the graphene-oxide / chitosan adjuvant so as to obtain a graphene-oxide / chitosan virus vaccine; immunizing laying hens with the graphene-oxide / chitosan virus vaccine as an immunogen, collecting eggs laid by the immunized laying hens,separating yolk from egg white, extracting yolk antibodies, and carrying out purification so as to obtain yolk antibodies of the Chinese Sacbrood Bee Virus; and then, mixing the yolk antibodies withan algae extract so as to obtain the yolk antibody compound preparation capable of resisting the Chinese Sacbrood Bee Virus. The yolk antibody compound preparation capable of resisting the Chinese Sacbrood Bee Virus has the advantages of being high in biological potency, good in prevention and treatment effects, free of any toxic or side residues, high in specificity, fast to effect, long-lastingin effect time and so on.

Description

technical field [0001] The invention belongs to the technical field of animal biological products, and in particular relates to a compound preparation of egg yolk antibody against Chinese bee cystic larvae disease virus and its preparation. Background technique [0002] As a precious resource, Apis cerana has made great contributions to honey production and global food production, and has promoted the development of the global economy to a certain extent. Bees also play an extremely important role in maintaining the ecological balance of animals and plants. Since Chinese Sacbrood Bee Virus (CSBV) mainly infects 1 to 3-day-old larvae, the infection rate and mortality rate of the larvae are extremely high, eventually resulting in the death of a large area of ​​the bee colony, bringing great harm to the bee industry. huge economic losses. CSBV is a genus of the family Infectious Soft Rot Virus. Its diameter is 26-30 nm. It is an icosahedral, non-enveloped, positive-strand RNA...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C07K16/02A61K39/395A61K47/46A61P31/14
Inventor 冯书营杜景霞李爱芳李艳胡丽娜叶晓君于梦园焦燕李静雅张海州杨金芳
Owner 洛阳蜂藻康生物科技有限公司