Rice bran extract and preparation method and application thereof to health food
A technology of rice bran extract and health food, which is applied in the field of rice bran extract and its preparation, can solve the problems of no extraction of biologically active ingredients, improvement of memory function without rice bran extract, etc., to achieve enhanced immunity, low requirements for preparation equipment, The effect of preventing heart disease
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Embodiment 1
[0030] The preparation method of the rice bran extract of the present embodiment comprises the following steps:
[0031] (1) Rice bran grinding: Grinding rice bran into rice bran powder, passing through a 60-mesh sieve to obtain coarse rice bran powder.
[0032] (2) Ethanol extraction: Weigh 2.000g of coarse rice bran powder with an analytical balance into a 250ml conical flask with stopper, add 80ml of 75% ethanol solution for extraction for 1.5h.
[0033] (3) Enzyme-assisted extraction: heat in a 60°C water bath, and when the temperature of the liquid in the conical flask reaches 60°C, add cellulase with a mass ratio of 5% rice bran powder, stir well, and use HCl standard solution and NaOH standard solution to adjust the pH value to 7.0, 60 ° C water bath for 1.5 h; place the Erlenmeyer flask in a water bath at 90 ° C for 15 min to inactivate the enzyme.
[0034] (4) Ultrasonic-assisted extraction: the rice bran solution after the water bath was placed in an ultrasonic clea...
Embodiment 2
[0039] The preparation method of the rice bran extract of the present embodiment comprises the following steps:
[0040] (1) Rice bran grinding: Grinding rice bran into rice bran powder, passing through a 60-mesh sieve to obtain coarse rice bran powder.
[0041] (2) Ethanol extraction: Weigh 2.000g of coarse rice bran powder with an analytical balance into a 250ml conical flask with stopper, add 80ml of ethanol solution with a volume concentration of 74% for 1.5h.
[0042] (3) Enzyme-assisted extraction: heat in a 60°C water bath, and when the temperature of the liquid in the conical flask reaches 60°C, add cellulase with a mass ratio of 4% rice bran powder, stir well, and use HCl standard solution and NaOH standard solution to adjust the pH value to 7.0, 60 ° C water bath for 1.5 h; place the Erlenmeyer flask in a water bath at 90 ° C for 15 min to inactivate the enzyme.
[0043] (4) Ultrasonic-assisted extraction: the rice bran solution after the water bath was placed in an...
Embodiment 3
[0048] The preparation method of the rice bran extract of the present embodiment comprises the following steps:
[0049] (1) Rice bran grinding: Grinding rice bran into rice bran powder, passing through a 60-mesh sieve to obtain coarse rice bran powder.
[0050] (2) Ethanol extraction: Weigh 2.000g of coarse rice bran powder with an analytical balance into a 250ml conical flask with a stopper, add 100ml of ethanol solution with a volume concentration of 77% for 1.5h.
[0051] (3) Enzyme-assisted extraction: heat in a 60°C water bath, and when the temperature of the liquid in the conical flask reaches 60°C, add cellulase with a mass ratio of 6% rice bran powder, stir well, and use HCl standard solution and NaOH standard solution to adjust the pH value to 7.0, 60 ° C water bath for 1.5 h; place the Erlenmeyer flask in a water bath at 90 ° C for 15 min to inactivate the enzyme.
[0052] (4) Ultrasonic-assisted extraction: the rice bran solution after the water bath was placed in...
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