Interaction factor gene Actin for zinc finger binding protein of corn, and recombinant expression vector and application thereof

A technology for binding protein and zinc finger protein, which can be used in application, genetic engineering, plant genetic improvement, etc., and can solve the problems of low AOS content, instability, difficulty in content determination and tissue localization, etc.
CN109912704AActive Publication Date: 2019-06-21UNIV OF JINAN
5 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
UNIV OF JINAN
Publication Date
2019-06-21

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the field of biotechnology, and particularly relates to an interaction factor gene Actin for zinc finger binding protein of corn, and a recombinant expression vector and application thereof. The full length of the gene is 12712 bp, and the gene is a complete expression frame. The gene comprises 6 exons and is encoded with 123 amino acids. Through expression detection in yeast, it is found that a Zm-Actin gene and a Zm-ZnRing gene can have obviously mutual regulation effect and play a role in chloroplasts through co-localization. The gene Actin has a function of improving the translation efficiency of foreign genes, the zinc finger binding protein has the activity of E3 ubiquitin ligase, the E3 ubiquitin ligase involves in a salt stress response process of plants, and the growth and metabolism of cells are regulated through control of membrane transport and generation of reactive oxygen; the gene can be applied to increase the reactive oxygen in tobacco leaves so as to regulate the growth and metabolism of the cells.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to the gene Actin, a maize zinc finger binding protein interaction factor gene, and its recombinant expression vector and application. Background technique

[0002] The E3 ubiquitination ligase that catalyzes the terminal process in the protein ubiquitination pathway of many RING-type zinc finger proteins contains a RING domain. These RING-type zinc finger proteins mainly participate in the ubiquitination through the interaction with other proteins Degradation of over-ubiquitinated proteins will change protein activity and affect biological effects. RING-type zinc finger proteins may be involved in many important biological effects. RING-type zinc finger protein genes are also closely related to salt stress. Zinc finger binding protein also has E3 ubiquitin ligase activity, participates in the process of plant salt stress response, mainly regulates the expression of genes r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More