Medium for promoting hydrogen production of HAU-M1 photosynthetic bacteria flora and application thereof

A technology of HAU-M1, photosynthetic bacteria, applied in bacteria, microorganism-based methods, and adding compounds to stimulate growth, etc., can solve problems such as low hydrogen production efficiency

Active Publication Date: 2019-06-25
HENAN AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current problem is that when corn stalks are used as substrates for synchronous saccharification and fe

Method used

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  • Medium for promoting hydrogen production of HAU-M1 photosynthetic bacteria flora and application thereof
  • Medium for promoting hydrogen production of HAU-M1 photosynthetic bacteria flora and application thereof
  • Medium for promoting hydrogen production of HAU-M1 photosynthetic bacteria flora and application thereof

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Experimental program
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Embodiment

[0036] In order to obtain a medium that can improve the hydrogen production capacity of HAU-M1 photosynthetic bacteria, the applicant chose to change the amino acid composition in the medium to investigate the hydrogen production changes during the fermentation process. The added amino acids were L-cysteine, L- One of leucine, L-alanine, L-threonine, L-serine;

[0037] In order to comprehensively investigate the effect of amino acid concentration on the hydrogen production capacity of HAU-M1, the applicant conducted a comparative experiment under different conditions for different amino acids. Among them, when adding L-cysteine, L-alanine and L-threo When L-leucine is added, the added concentrations are 0.3 g / L, 0.6 g / L, 0.9 g / L, 1.2 g / L; when L-leucine is added, the added concentrations are 0.6 g / L, 0.9 g / L , 1.2 g / L, 1.5 g / L; when L-serine was added, the added concentrations were 1 g / L, 1.5 g / L, 2 g / L, 2.5 g / L; the control group did not add hydrogen-producing amino acids; E...

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Abstract

The invention belongs to the technical field of microbial fermentation biological hydrogen production, and particularly relates to a medium for promoting hydrogen production of HAU-M1 photosynthetic bacteria flora and application thereof. The medium comprises 0.1-1g/L of NH4Cl, 0.01-0.5g/L of MgCl2, 0.01-0.2g/L of yeast extract, 0.1-1g/L of K2HPO4, 1-3g/L of NaCl, 1-5g/L of sodium glutamate and 0.3-2.5g/L of hydrogen-producing amino acid. The hydrogen-producing amino acid is one or a mixture of a plurality of L-cysteine, L-alanine, L-threonine, L-leucine and L-serine in any ratio. Based on a general inventive concept, the invention also provides application of the medium in biological hydrogen production, thereby effectively improving the hydrogen production capability of the HAU-M1 photosynthetic bacteria flora.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation biological hydrogen production, and in particular relates to a culture medium for promoting hydrogen production of HAU-M1 photosynthetic bacterial flora and its application. Background technique [0002] Hydrogen energy is considered to be the most ideal alternative energy source in the future because of its high combustion calorific value, cleanness and pollution-free characteristics. Among many hydrogen production technologies, biological hydrogen production has attracted more and more attention because of its high efficiency, greenness and low cost. . Research on biological hydrogen production at home and abroad mainly focuses on two aspects: anaerobic dark fermentation hydrogen production and photofermentation hydrogen production. Among them, photosynthetic biological hydrogen production technology uses photosynthetic bacteria to convert straw, sludge, and feces as raw materials...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P3/00C12N1/38C12N1/20C12R1/01
CPCY02E50/30
Inventor 张全国夏晨曦杨雪梅路朝阳胡建军王艳锦王毅张志萍
Owner HENAN AGRICULTURAL UNIVERSITY
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