Application of transcriptional sequencing technology in screening jujube shrink disease anti-disease gene

Abstract

The invention discloses application of a transcriptional sequencing technology in screening a jujube shrink disease anti-disease gene and relates to the technical field of biological genes. The jujubeshrink disease is caused by A. altrenata by dip dyeing. The varieties of jujube are Fengmiguan resisting the jujube shrink disease and Qiyuexian infected with the jujube shrink disease. The transcriptional sequencing technology includes pretreatment, transcriptome sequencing, and data processing. The pretreatment includes extraction of total RNA from jujube, detection of total RNA quality, synthesis of a cDNA library and real-time quantitative PCR verification. The Fengmiguan resisting the jujube shrink disease and Qiyuexian infected with the jujube shrink disease and clear water are adoptedfor treatment of reference materials, A. altrenata is used as an induction condition, a response mechanism of jujube under the induction of A. altrenata is studied at the molecular level by using thetranscriptional sequencing technology and a qPCR technology and other technologies, so as to screen the jujube shrink disease anti-disease gene and provide a certain theoretical basis for jujube breeding work.

Description

technical field [0001] The invention relates to the technical field of biological genes, in particular to the application of a transcription sequencing technology in screening jujube fruit shrinkage disease resistance genes. Background technique [0002] Jujube (Ziziphus jujuba Mill.) is a plant of the genus Jujube in the Rhamnaceae family. It is an important dry fruit tree species in my country. The development of the jujube industry plays a very positive role in bringing high-level economic income to farmers and promoting a good change in the ecological environment. (Liu Mengjun, 2009) species. Jujube trees are widely used in promoting the development of farmers' economy and improving the ecological environment because of their special products in my country, drought resistance and ridge tolerance, fast abundance of jujube fruits, rich nutrition, especially high vitamin content, and can be used as medicine to moisten the heart and lungs, nourish qi and nourish the body. occ...

AI Technical Summary

Problems solved by technology

[0003] The rapid development of the jujube industry plays an important role in promoting the development of the peasant economy and improving the ecological environment. However, due to the occurrence of the jujube fruit shrinkage disease, the jujube fruit falls off and dies, which seriously affects the yield and quality of the jujube fruit. cause serious harm to the production of the disease, the prevention and control of the disease is urgent

In the 1970s, there was a report on jujube fruit shrinkage disease research in China (Han Jinsheng 1979), but there is no unified conclusion for the identification of jujube fruit shrinkage disease pathogenic bacteria at present

Although reports on the pathogens of jujube fruit shrinkage disease vary from region to region, it is widely believed that jujube fruit shrinkage disease is caused by the above-mentioned single pathogen or mixed infection of two or more pathogens, and it was found that the strain of Alternaria may be the cause of jujube fruit shrinkage. The most common pathogenic fungus of fruit shrinkage disease

[0004] In order to control the above-mentioned pathogens, relevant research mainly explores the defense effect of jujube on Alternaria tennatus from the physiological, biochemical and morphological levels, but there are few reports on the molecular level.

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