Macrophages membrane coated breast cancer targeted nanoparticles and preparation method thereof

A technology for macrophages and breast cancer, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc. It can solve the problems of difficulty in loading drugs and lack of release mechanism, and achieve the preparation process Simple and feasible, improve the killing ability and improve the effect of treatment effect

Inactive Publication Date: 2019-07-02
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the lack of a release mechanism in traditional biofilm-coated nano drug delivery systems, when recruited or adhered

Method used

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  • Macrophages membrane coated breast cancer targeted nanoparticles and preparation method thereof
  • Macrophages membrane coated breast cancer targeted nanoparticles and preparation method thereof
  • Macrophages membrane coated breast cancer targeted nanoparticles and preparation method thereof

Examples

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Example Embodiment

[0025] Example 1: Macrophage membrane-coated nanoparticle preparation loaded with paclitaxel, the preparation method of which is as follows figure 1 shown, including the following steps:

[0026] (1) Prepare 50 μL of polyethylene glycol-polyamino acid N,N-dimethylformamide solution with a volume concentration of 10 mg / mL, add 0.1 mg of the original drug of paclitaxel, mediate and mix until completely dissolved, and centrifuge to collect the solution;

[0027] (2) The modified poly-β-amino ester paclitaxel N,N-dimethylformamide solution in step (1) is slowly added dropwise from a high place to the injection vehicle under the condition of rapid stirring, and at 25°C After stirring for 15 minutes, the injection medium was dialyzed for 48 hours to obtain paclitaxel-loaded nanoparticles.

[0028] (3) Extract peritoneal macrophages, inject 1 mL of 4% thioglycolate broth into the abdominal cavity of 25 g male mice, kill the mice after 3 days, immerse the whole body in 75% ethanol fo...

Example Embodiment

[0031] Example 2: pH-sensitive macrophage membrane-coated nanoparticle formulation loaded with paclitaxel

[0032] (1) Prepare 50 μL of a modified poly-β-amino ester N,N-dimethylformamide solution with a volume concentration of 10 mg / mL, add 0.2 mg of the original drug of paclitaxel, mediate and mix until completely dissolved, and centrifuge to collect the liquid;

[0033] (2) The modified poly-β-amino ester paclitaxel N,N-dimethylformamide solution in step (1) is slowly added dropwise from a high place to the injection vehicle under the condition of rapid stirring, and at 20°C After stirring for 30 minutes, dialyze with injection solvent for 12 hours to obtain paclitaxel-loaded nanoparticles;

[0034] (3) Extract peritoneal macrophages, inject 1 mL of 4% thioglycolate broth into the abdominal cavity of 25 g male mice, kill the mice after 3 days, immerse the whole body in 75% ethanol for disinfection, and inject 5 mL of medium twice to extract peritoneal macrophages Cells wer...

Example Embodiment

[0037] Example 3: Preparation of near-infrared probe-labeled macrophage membrane-coated nanoparticle preparations

[0038] (1) Prepare 50 μL of a modified poly-β-amino ester N,N-dimethylformamide solution with a volume concentration of 10 mg / mL, add 10 μg of a near-infrared probe, mediate and mix until completely dissolved, and centrifuge to collect the liquid;

[0039] (2) The N, N-dimethylformamide solution of the modified poly-β-amino ester near-infrared probe of step (1) is slowly added dropwise from a height to the injection solvent under the condition of rapid stirring, at 20°C After stirring for 30 minutes under the same conditions, the injection solvent was dialyzed for 24 hours to obtain nanoparticles loaded with near-infrared probes;

[0040] (3) Extract peritoneal macrophages, inject 1 mL of 4% thioglycolate broth into the abdominal cavity of 25 g male mice, kill the mice after 3 days, immerse the whole body in 75% ethanol for disinfection, and inject 5 mL of medium...

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Abstract

The invention belongs to the field of pharmaceutical preparations, and relates to macrophages membrane coated breast cancer targeted nanoparticles and a preparation method thereof. The macrophage membrane coated paclitaxel-loaded nanoparticles are prepared into a paclitaxel-loaded nanometer medicine delivery system from paclitaxel, macrophage membranes, amphiphilic high molecular materials, an injection solvent and the like. The macrophage membrane coated medicine carrying nanoparticles are good in biocompatibility, simple in preparation method and uniform in nanoparticle size distribution. Tumor cells are actively recognized through biological molecules on the surface of the macrophage membranes, so that active collection of medicine-containing carriers to a tumor region is effectively increased, retention of the medicine-containing carriers in tumor tissue is increased, controllability of medicine release is realized, and the anti-tumor treatment effects of medicines are increased.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to a macrophage membrane-coated paclitaxel-loaded breast cancer targeting nanoparticle and a preparation method thereof. Background technique [0002] The prior art discloses that breast cancer is one of the cancers with the highest morbidity and mortality among women in my country. The number of new breast cancer cases and deaths in China account for 12.2% and 9.6% of the world's total each year, and it also occurs in men, seriously affecting National health and quality of life. In clinical practice, common treatment methods for breast cancer include surgical resection, radiotherapy and chemotherapy. However, due to the high degree of metastasis of triple-negative breast cancer to the bone, liver, and lung, the prognosis is poor, and surgery cannot completely remove the malignant tissue. Due to the current limitations of breast cancer typing and molecular scre...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K31/337A61K47/46A61P35/00
CPCA61K9/5146A61K9/5176A61K31/337
Inventor 蒋晨张宇
Owner FUDAN UNIV
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