Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Macrophages membrane coated breast cancer targeted nanoparticles and preparation method thereof

A technology for macrophages and breast cancer, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc. It can solve the problems of difficulty in loading drugs and lack of release mechanism, and achieve the preparation process Simple and feasible, improve the killing ability and improve the effect of treatment effect

Inactive Publication Date: 2019-07-02
FUDAN UNIV
View PDF2 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of a release mechanism in traditional biofilm-coated nano drug delivery systems, when recruited or adhered to tumor tissue through different mechanisms, it is difficult for the loaded drug to be quickly released and absorbed by tumor cells.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Macrophages membrane coated breast cancer targeted nanoparticles and preparation method thereof
  • Macrophages membrane coated breast cancer targeted nanoparticles and preparation method thereof
  • Macrophages membrane coated breast cancer targeted nanoparticles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Macrophage membrane-coated nanoparticle preparation loaded with paclitaxel, the preparation method of which is as follows figure 1 shown, including the following steps:

[0026] (1) Prepare 50 μL of polyethylene glycol-polyamino acid N,N-dimethylformamide solution with a volume concentration of 10 mg / mL, add 0.1 mg of the original drug of paclitaxel, mediate and mix until completely dissolved, and centrifuge to collect the solution;

[0027] (2) The modified poly-β-amino ester paclitaxel N,N-dimethylformamide solution in step (1) is slowly added dropwise from a high place to the injection vehicle under the condition of rapid stirring, and at 25°C After stirring for 15 minutes, the injection medium was dialyzed for 48 hours to obtain paclitaxel-loaded nanoparticles.

[0028] (3) Extract peritoneal macrophages, inject 1 mL of 4% thioglycolate broth into the abdominal cavity of 25 g male mice, kill the mice after 3 days, immerse the whole body in 75% ethanol fo...

Embodiment 2

[0031] Example 2: pH-sensitive macrophage membrane-coated nanoparticle formulation loaded with paclitaxel

[0032] (1) Prepare 50 μL of a modified poly-β-amino ester N,N-dimethylformamide solution with a volume concentration of 10 mg / mL, add 0.2 mg of the original drug of paclitaxel, mediate and mix until completely dissolved, and centrifuge to collect the liquid;

[0033] (2) The modified poly-β-amino ester paclitaxel N,N-dimethylformamide solution in step (1) is slowly added dropwise from a high place to the injection vehicle under the condition of rapid stirring, and at 20°C After stirring for 30 minutes, dialyze with injection solvent for 12 hours to obtain paclitaxel-loaded nanoparticles;

[0034] (3) Extract peritoneal macrophages, inject 1 mL of 4% thioglycolate broth into the abdominal cavity of 25 g male mice, kill the mice after 3 days, immerse the whole body in 75% ethanol for disinfection, and inject 5 mL of medium twice to extract peritoneal macrophages Cells wer...

Embodiment 3

[0037] Example 3: Preparation of near-infrared probe-labeled macrophage membrane-coated nanoparticle preparations

[0038] (1) Prepare 50 μL of a modified poly-β-amino ester N,N-dimethylformamide solution with a volume concentration of 10 mg / mL, add 10 μg of a near-infrared probe, mediate and mix until completely dissolved, and centrifuge to collect the liquid;

[0039] (2) The N, N-dimethylformamide solution of the modified poly-β-amino ester near-infrared probe of step (1) is slowly added dropwise from a height to the injection solvent under the condition of rapid stirring, at 20°C After stirring for 30 minutes under the same conditions, the injection solvent was dialyzed for 24 hours to obtain nanoparticles loaded with near-infrared probes;

[0040] (3) Extract peritoneal macrophages, inject 1 mL of 4% thioglycolate broth into the abdominal cavity of 25 g male mice, kill the mice after 3 days, immerse the whole body in 75% ethanol for disinfection, and inject 5 mL of medium...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The average particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of pharmaceutical preparations, and relates to macrophages membrane coated breast cancer targeted nanoparticles and a preparation method thereof. The macrophage membrane coated paclitaxel-loaded nanoparticles are prepared into a paclitaxel-loaded nanometer medicine delivery system from paclitaxel, macrophage membranes, amphiphilic high molecular materials, an injection solvent and the like. The macrophage membrane coated medicine carrying nanoparticles are good in biocompatibility, simple in preparation method and uniform in nanoparticle size distribution. Tumor cells are actively recognized through biological molecules on the surface of the macrophage membranes, so that active collection of medicine-containing carriers to a tumor region is effectively increased, retention of the medicine-containing carriers in tumor tissue is increased, controllability of medicine release is realized, and the anti-tumor treatment effects of medicines are increased.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to a macrophage membrane-coated paclitaxel-loaded breast cancer targeting nanoparticle and a preparation method thereof. Background technique [0002] The prior art discloses that breast cancer is one of the cancers with the highest morbidity and mortality among women in my country. The number of new breast cancer cases and deaths in China account for 12.2% and 9.6% of the world's total each year, and it also occurs in men, seriously affecting National health and quality of life. In clinical practice, common treatment methods for breast cancer include surgical resection, radiotherapy and chemotherapy. However, due to the high degree of metastasis of triple-negative breast cancer to the bone, liver, and lung, the prognosis is poor, and surgery cannot completely remove the malignant tissue. Due to the current limitations of breast cancer typing and molecular scre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K9/51A61K31/337A61K47/46A61P35/00
CPCA61K9/5146A61K9/5176A61K31/337
Inventor 蒋晨张宇
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com