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Application of bacteroides fragilis extract to preparation of composition for preventing and treating psoriasis

A technology of Bacteroides fragilis and extract, applied in the application field of the composition, can solve the problems of strong toxicity restricting long-term and lasting treatment, unsatisfactory medical needs, short effective period of clinical symptom treatment, etc., and achieves good food and medicinal use. Foreground effect

Active Publication Date: 2019-07-02
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Regardless of the treatment method, its strong toxicity limits its long-term and durable treatment, resulting in a short effective period of treatment for clinical symptoms
Therefore, there remains an unsatisfactory medical need in the treatment and prevention of relapse

Method used

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  • Application of bacteroides fragilis extract to preparation of composition for preventing and treating psoriasis
  • Application of bacteroides fragilis extract to preparation of composition for preventing and treating psoriasis
  • Application of bacteroides fragilis extract to preparation of composition for preventing and treating psoriasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The preparation of embodiment 1 Bacteroides fragilis extract

[0068] (1) Fermentation culture of Bacteroides fragilis

[0069] Streak inoculation of strains on blood plate, anaerobic culture for 48h. Observe the colony morphological characteristics, staining characteristics, size, club shape and distribution, etc.

[0070] Colony characteristics: Bacteroides fragilis ZY-312, after being cultured on a blood plate for 48 hours, is slightly convex, translucent, white, smooth, non-hemolytic, and the diameter of the colony is between 1-3mm. See figure 1 .

[0071] Morphology under the microscope: Bacteroides fragilis ZY-312 was examined by Gram staining. It is a Gram-negative bacterium with a typical rod shape, blunt rounded ends and dense staining. The uncolored part in the middle of the bacteria is like a vacuole. figure 2 .

[0072] A single colony was selected and inoculated in tryptone broth for fermentation and culture for 8 hours (at a temperature of 37° C.). Th...

Embodiment 2

[0084] Example 2 Curative effect of different doses of Bacteroides fragilis capsular polysaccharide A on imiquimod-induced psoriasis in mice

[0085] 1. Experimental design

[0086] This example induces the female BALB / c mouse psoriasis model by 5% imiquimod, and then uses the Bacteroides fragilis extract (the main component is capsular polysaccharide A) prepared in Example 1 to treat 5% imiquimod Specially induce psoriasis in female BALB / c mice for treatment, and detect its therapeutic effect. In this example, capsular polysaccharide A with a molecular weight of 70KD is taken as an example to detect the therapeutic effects of low, medium and high doses of capsular polysaccharide A on psoriasis.

[0087] Mouse psoriasis induction method: 60 female BALB / c mice were used in this example, and each experimental mouse was assigned a unique number. Prior to grouping animals, the item number, species / strain, sex, cage number, and animal number should be marked on the cage label. Usi...

Embodiment 3

[0112] Example 3 The curative effect of Bacteroides fragilis capsular polysaccharide A with different molecular weights on psoriasis

[0113] This embodiment refers to the method of Example 2 to prepare a mouse psoriasis animal model, and then use the 2KD, 5KD, 40KD, 70KD, 110KD Bacteroides fragilis capsular polysaccharide A prepared in Example 1 of the present invention to 5% mirin Prevent / treat psoriasis in mice induced by quimod, and detect the therapeutic effect of different molecular weight Bacteroides fragilis capsular polysaccharide A on psoriasis. This embodiment takes high doses of Bacteroides fragilis capsular polysaccharide A of 2KD, 5KD, 40KD, 70KD and 110KD as an example.

[0114] Referring to the grouping method of the experimental group in Example 2, the mice were divided into normal control group, model group, 2KD group, 5KD group, 40KD group, 70KD group and 110KD group.

[0115] Dosing regimen refers to Example 2.

[0116] The specific experimental grouping ...

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Abstract

The invention relates to an application of a bacteroides fragilis extract to preparation of a composition for preventing and treating psoriasis. The bacteroides fragilis extract contains bacteroides fragilis capsular polysaccharide A. The inventor of the application disclosed by the invention finds that the bacteroides fragilis extract containing the capsular polysaccharide A has the function of preventing / treating the psoriasis.

Description

technical field [0001] The invention relates to the technical field of application of Bacteroides fragilis, in particular to the application of an extract of Bacteroides fragilis in preparing a composition for preventing and treating psoriasis. Background technique [0002] Psoriasis is an autoimmune skin disease regulated by genetic genes and mediated by T lymphocytes. The main histopathological changes are abnormal proliferation of keratinocytes, parakeratosis, hyperkeratosis, angiogenesis and inflammatory cell infiltration. Its etiology and pathogenesis are not yet fully clear, and there is no effective cure. It is one of the most common chronic inflammatory hyperplastic skin diseases. The disease is stubborn and easy to relapse, which can cause systemic damage and seriously affect the quality of life and even the survival cycle of patients. [0003] Psoriasis is a complex genetic disorder of immune dysfunction, although the mechanisms of inheritance are not fully defin...

Claims

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Application Information

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IPC IPC(8): A61K35/741A23L33/135C08B37/00A61P17/06
CPCA23V2002/00A61K35/741A23L33/135C08B37/0003A23V2200/318
Inventor 智发朝王晔刘洋洋李平白杨邝高波郑丽君
Owner GUANGZHOU ZHIYI PHARMA INC
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