Centrifugal microfluidic chip and nucleic acid analysis system

Abstract

The invention relates to a centrifugal microfluidic chip and a nucleic acid analysis system, wherein the centrifugal microfluidic chip includes a chip substrate, a magnetic adsorption component and amagnetic component; the chip substrate is also provided with a stirring chamber and a liquid injection hole connected with the stirring chamber; the magnetic adsorption component is accommodated in the stirring chamber; the chip substrate has a rotating center, and the magnetic component is arranged outside the chip substrate and is located at a position, close to the rotating center, of the stirring chamber. On one hand, when the magnetic adsorption component moves to a position away from the magnetic component, and under the action of centrifugal force, the magnetic adsorption component moves to the position, far from the rotating center, of the stirring chamber; on the other hand, when the magnetic adsorption component moves to a position close to the magnetic component, the magnetic adsorption component moves to a position, close to the rotating center, of the stirring chamber under the action of magnetic adsorption. An ideal mixing effect can be achieved, and a certain cracking effect is achieved and is stable and effective on the premise of simplifying the structure and needing no complicated instruments and equipment.

Description

technical field [0001] The present application relates to the field of centrifugal microfluidics, in particular to a centrifugal microfluidic chip and a nucleic acid analysis system. Background technique [0002] Microfluidics refers to the manipulation of liquids on the submillimeter scale, where the submillimeter scale is generally several microns to hundreds of microns. Microfluidic technology integrates the basic operating units involved in the fields of biology and chemistry, and even the functions of the entire laboratory, including sampling, dilution, reaction, separation, detection, etc., on a small chip, so it is also called lab-on-a-chip ( Lab-on-a-Chip). This kind of chip is generally composed of various liquid storage pools and interconnected microchannel networks, which can greatly shorten the sample processing time, and realize the maximum utilization efficiency of reagent consumables through precise control of liquid flow. [0003] Microfluidic systems are d...

AI Technical Summary

Problems solved by technology

The first method of mixing often puts forward greater requirements on the centrifugal motor. At the same time, since the rotational speed in centrifugal microfluidics is the driving force for manipulating the flow of liquid on the entire chip, changing the rotational speed will inevitably affect other parts of the microfluidic chip. The flow of liquid reagents affects the design of the chip, which increases the difficulty

The second method will increase the processing difficulty of the entire microfluidic chip, and at the same time, the mixing effect of the continuously bent serpentine flow pipe is often not so ideal

In centrifugal microfluidics, these physical methods tend to greatly increase the complexity of the instrument

Moreover, since the microfluidic chip is in a centrifugal state during the operation, it is often difficult for the ultrasonic probe to make direct contact with the microfluidic chip, and the cracking effect is difficult to meet expectations.

On the other hand, it is difficult to align the laser spot with the cracking chamber of the microfluidic chip when the laser irradiation method is used for cracking.

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