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Application of mmu-mir-183-5p in the preparation of drugs for inhibiting embryo implantation

A drug and embryo technology is applied in the application field of mmu-miR-183-5p in the preparation of drugs for inhibiting embryo implantation, which can solve problems such as no documented effect and achieve a significant inhibitory effect.

Active Publication Date: 2021-08-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the role of miR-183 in tumor cell migration, differentiation, proliferation, and microvascular formation has been confirmed, there is no literature to confirm its role in embryo implantation

Method used

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  • Application of mmu-mir-183-5p in the preparation of drugs for inhibiting embryo implantation
  • Application of mmu-mir-183-5p in the preparation of drugs for inhibiting embryo implantation
  • Application of mmu-mir-183-5p in the preparation of drugs for inhibiting embryo implantation

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Experimental program
Comparison scheme
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Embodiment 1

[0026] 1. Small RNA sequencing

[0027] 1. Take materials

[0028] For the ICR mouse strain, select the estrous female mouse and the male mouse to co-cage at 17 o'clock in the evening, and check the plug at 9 o'clock the next morning. If a white plug appears at the vaginal opening of the female mouse, it proves that the female mouse is pregnant, and it is recorded as pregnancy D1 sky.

[0029] Collect endometrial tissue from the first day (D1), fourth day (D4), fifth day implantation site (D5IMS) and non-implantation site (D5IIS) after pregnancy: the collection method is, within the corresponding days, At 10:00 in the morning, the mice were humanely killed by neck dissection, and 75% alcohol was used for disinfection, and then the uterine tissue of the mice was removed, the adherent fat and blood vessels were removed, and the attached blood was washed away, and the mice were separated by extrusion stripping endometrium. The endometrial tissue of the first day (D1), fourth d...

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Abstract

The invention discloses the application of a microRNA—mmu-miR-183-5p in the preparation of medicines for inhibiting embryo implantation. The present invention screens out the microRNA that regulates the uterine receptivity stage and plays a key role in the implantation site through the microarray experiment of microRNA expression profile and bioinformatics method: mmu‑miR‑183‑5p, and it is proved by experiments that mmu‑miR‑183 ‑5p has an extremely significant inhibitory effect on embryo implantation, suggesting that it can be used as a contraceptive drug and provides a new way for new drug screening.

Description

[0001] (1) Technical field [0002] The invention relates to the application of mmu-miR-183-5p in the preparation of medicines for inhibiting embryo implantation. [0003] (2) Background technology [0004] The miR-183 family consists of three miRNAs, miR-96, miR-182 and miR-183, which are highly conserved in structure. Through miRBase query comparison, it was found that miR-183-5p is highly conserved in different species, and the identity of human miR-183-5p and mouse miR-183-5p reached 100%. This family is abnormally expressed in various tumors of the digestive system, genitourinary system, and respiratory system. Reported studies have confirmed that miR-183 can promote cell migration, invasion, differentiation, proliferation, and indirectly affect the formation of microvessels. In the process of embryo implantation, physiological processes such as migration, differentiation, proliferation, and microvascular formation also occur. Endometrial epithelial cells and embryos wil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/713A61K47/54A61K47/52A61P15/18
CPCA61K31/713A61K47/52A61K47/54A61K47/554A61P15/18
Inventor 王争光曹丁壬梁晶婕邱寒峰谭强施爽
Owner ZHEJIANG UNIV