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Vertical type rotary tank bioreactor for large-scale cell culturing

A bioreactor and large-scale culture technology, applied in the field of vertical rotating tank bioreactor, can solve the problem of rarely harvesting cells, and achieve the effect of avoiding pollution and saving production links

Pending Publication Date: 2019-07-16
南京比瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional bioreactors can only cultivate cells to harvest proteins, and are rarely used to harvest cells

Method used

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  • Vertical type rotary tank bioreactor for large-scale cell culturing
  • Vertical type rotary tank bioreactor for large-scale cell culturing
  • Vertical type rotary tank bioreactor for large-scale cell culturing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] figure 1 It is a schematic diagram of the overall internal structure of the present invention. It can be seen in conjunction with the accompanying drawings that the rotary bioreactor for large-scale cultivation of the present invention includes a cultivation tank 6. In practical applications, the cultivation tank 6 is placed vertically. Culture cells in. During the cultivation process, the upper stirring motor drives the stirring paddle to rotate at a low speed to stir the culture medium so that the culture medium is evenly mixed. The bottom of the culture tank 6 is provided with a rotary drive device, which can drive the culture tank 6 to rotate at a high speed along its vertical central axis to generate centrifugal force, which can separate, concentrate and wash the cells. Driving the rotation of the culture tank 6 is not present in any culture tank 6, and the present invention combines the functions of the culture tank and the centrifuge, which is one of the most im...

Embodiment 2

[0034] figure 2 It is a schematic example of the overall external structure of the present invention. As can be seen in conjunction with the accompanying drawings, the culture tank 6 is arranged in an incubator, and the side wall of the incubator is provided with an observation window 17. The box is also provided with a constant temperature system and a temperature display system. One side of the incubator is a door that can be rotated and opened, and a handle 19 is arranged on the door. The observation window 17 can be located on the door, or on other side walls of the incubator.

Embodiment 3

[0036] This example introduces in detail the specific means for achieving the culture tank 6 being driven to rotate by the bottom motor 2 and rotating at a high speed, and sealing the tank mouth. image 3 yes figure 1 In the partial enlarged view of B, it can be seen in conjunction with the accompanying drawings that the bottom of the culture tank 6 is provided with a cross groove 18, the rotation drive device includes a rotary tray 4 and a bottom motor 2, and the upper surface of the rotary tray 4 is provided with The cross protruding strips 22 intersect, the intersection point of the cross protruding strips 22 is located at the center of the rotating tray 4 , the rotating tray 4 is arranged in contact with the bottom of the thickened base, and the cross protruding strips 22 are inserted into the cross grooves 18 exactly. The intersection point of the cross groove 18 is located at the center of the thickened base. Inserting the cross protruding strip 22 into the cross groove...

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Abstract

The invention discloses a rotary bioreactor for large-scale cell culturing. The rotary bioreactor comprises a rotatable culture tank, wherein a tank cover is arranged at a tank opening of the culturetank in a sealing manner; a rotating driving device is arranged below the bottom of the culture tank, and can drive the culture tank to rotate along a vertical central axis of the culture tank; the tank cover of the culture tank is fixed and does not rotate; a plurality of interfaces are formed in the tank cover; a rotary joint is arranged between the tank cover and the tank opening, and is used for realizing the sealing rotation of the culture tank; and an electrode, a feeding and discharging pipeline, an on-line cell detecting system, a stirring system, a perfusion system and a ventilating system are also connected to the interfaces in the tank cover. The rotary bioreactor disclosed by the invention is suitable for the whole preparation process of a cell therapy preparation; and all preparation steps of cell separation, magnetic separation transfection, amplifying, concentrating, washing, configuring and subpackaging can be completed in a full-sealed full-automatic controlled bioreactor.

Description

technical field [0001] The invention relates to a vertical rotary tank bioreactor for large-scale cell culture, belonging to the technical field of cell culture and regenerative medicine. Background technique [0002] The in vitro culture of cells and tissues has become an essential part of life research and practice. Traditional bioreactors can only cultivate cells to harvest proteins, and are rarely used to harvest cells. With the rise of immunotherapy and stem cell therapy, large-scale, fully enclosed, fully automatic, and multifunctional cell preparation devices have become urgent. Contents of the invention [0003] In order to solve the above problems, the present invention discloses a rotary bioreactor for large-scale cultivation. [0004] Concrete scheme of the present invention is: [0005] A rotary bioreactor for large-scale cultivation, comprising a rotatable culture tank, the mouth of the culture tank is sealed with a tank cover; the bottom of the culture tan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/34C12M1/04C12M1/02
CPCC12M27/10C12M27/02C12M29/06C12M41/12
Inventor 朱学仁朱羚
Owner 南京比瑞生物科技有限公司
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