New method for producing low polyxylose by utilizing microorganism

A technology of xylo-oligosaccharides and microorganisms, applied in the field of microbial enzyme engineering technology, can solve the problems of low enzyme activity that cannot meet industrial production, increase the difficulty of production technology, and long time, so as to improve animal production performance and increase the additional value of development and utilization. The effect of low value and low cost

Inactive Publication Date: 2010-12-15
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

In recent years, it has been reported to use Aspergillus niger to produce xylanase by solid fermentation, and then use xylanase to hydrolyze xylan to prepare xylooligosaccharides. However, this method needs to be cultivated at 28°C for 72 hours to obtain enzyme activity greater than 2500IU/ g (medium dry weight) xylanase, not only takes a long time, but

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] 1. Separation and screening of Aspergillus niger U8-7

[0033] (1) Separation and purification of strains

[0034] Obtain the Aspergillus niger strain from the decaying corncob in the field, take 1g of its sample, put it into an Erlenmeyer flask with 100ml sterile water and glass beads, shake to disperse it, make a corncob suspension, and insert it into PDA medium (potato 200g, 20g glucose, 20g agar, 1000ml water, natural pH) culture at 30°C for 72 hours for separation and purification while producing spores. The control strain was Aspergillus niger AS3.4309, which was purchased from the Chinese Academy of Sciences Microbial Culture Collection.

[0035] (2) Strain propagation and cultivation

[0036] Scrape the purified Aspergillus niger spores with an inoculating loop in a triangular flask filled with 100ml sterile water and glass beads, shake, filter with 4 layers of gauze and dilute and count to make a spore suspension containing 3-5 spores per ml. Take 1ml of spore suspen...

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PUM

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Abstract

The invention relates to a new method for producing low polyxylose by utilizing microorganism, which takes corncob as a raw material. The method comprises the following steps: smashing corncob, adding water, and carrying out acidification and high-temperature splitting decomposition to obtain mixed liquor of the raw material; directly inoculating fermented culture of aspergillus niger U8-7, carrying out microbial induction enzyme production zymolysing, and then separating, purifying, decoloring, desalinating, filtering, concentrating and spray drying to obtain the low polyxylose. The method of the invention leaves out the production link for producing low polyxylose, reduces production cost, and obviously increases the output capacity of low polyxylose.

Description

technical field [0001] The invention belongs to the application field of microbial enzyme engineering technology and is used for producing xylooligosaccharides from corncobs, in particular to a new method for producing xylooligosaccharides by using microorganisms. Background technique [0002] Xylooligosaccharides, also known as xylooligosaccharides, are oligosaccharides composed of 2-7 xylose molecules connected by β-1, 4 glycosidic bonds, mainly xylobiose and xylotriose. Xylo-oligosaccharides occur naturally in bamboo roots, fruits, vegetables, milk and honey, but in very small amounts. The main raw materials used to produce xylooligosaccharides are hardwood, corn cob, sawdust, bagasse, sugar beet bagasse, chaff, bran, etc. The sweetness of xylo-oligosaccharide is about 40% of that of sucrose, with pure sweet taste, dry product is light brown or light yellow powder, does not degrade at PH 0.8-1.5, has high acid stability, does not absorb moisture and returns to moisture, ...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12P19/04C12N1/14C12R1/685
Inventor 张秀江王秋菊
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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