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Preparation method of autosoluble hyaluronic acid microneedles

A technology of hyaluronic acid and autolysis, which is applied in the field of preparation of autolyzed hyaluronic acid microneedles, can solve the problems of skin irritation, drug activity and the like

Pending Publication Date: 2019-07-19
南京医科大学康达学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many biological microneedles will add organic solvents during the production process, which may have a certain stimulating effect on the skin in later use. At the same time, some preparation conditions will also affect the activity of the drug itself

Method used

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  • Preparation method of autosoluble hyaluronic acid microneedles
  • Preparation method of autosoluble hyaluronic acid microneedles

Examples

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Effect test

Embodiment 1

[0038] A preparation method of self-dissolving hyaluronic acid microneedles, comprising the steps of:

[0039] (1) Take an appropriate amount of pure hyaluronic acid with a molecular weight of 5KDa, add purified water at a mass volume ratio (g / mL) of 1:50, swell in a water bath at 30°C for 30 minutes, and stand at room temperature to remove bubbles in the solution to obtain a uniform hyaluronic acid. Hyaluronic acid clarification solution, spare;

[0040] (2) Clean the PDMS (Polydimethylsiloxane) microneedle mold, air-dry, and set aside;

[0041] (3) Take the hyaluronic acid clarified solution in step (1), cast it onto the PDMS microneedle mold naturally, and shake it back and forth to ensure that it is evenly spread; the dosage of the above hyaluronic acid clarified solution is 0.12ml / cm 2 ;

[0042] (4) Put the PDMS microneedle mold coated with the clarified hyaluronic acid solution in a vacuum drying oven at 30°C, evacuate to 0.06MPa, hold the pressure for 2.5min, slowly ...

Embodiment 2

[0044] A preparation method of self-dissolving hyaluronic acid microneedles, comprising the steps of:

[0045] (1) Take an appropriate amount of pure hyaluronic acid with a molecular weight of 10KDa, add purified water at a mass volume ratio (g / mL) of 3:100, swell in a water bath at 35°C for 35 minutes, and stand at room temperature to remove bubbles in the solution to obtain a uniform hyaluronic acid. Hyaluronic acid clarification solution, spare;

[0046] (2) Clean the PDMS (Polydimethylsiloxane) microneedle mold, air-dry, and set aside;

[0047] (3) Take the hyaluronic acid clarified solution in step (1), cast it onto the PDMS microneedle mold naturally, and shake it back and forth to ensure that it is evenly spread; the dosage of the above hyaluronic acid clarified solution is 0.15ml / cm 2 ;

[0048] (4) Put the PDMS microneedle mold coated with the clarified hyaluronic acid solution in a vacuum drying oven at 30°C, evacuate to 0.06 MPa, hold the pressure for 3 minutes, a...

Embodiment 3

[0050] A preparation method of self-dissolving hyaluronic acid microneedles, comprising the steps of:

[0051] (1) Take an appropriate amount of pure hyaluronic acid with a molecular weight of 1KDa, add purified water at a mass volume ratio (g / mL) of 1:100, swell in a water bath at 25°C for 25 minutes, and stand at room temperature to remove bubbles in the solution to obtain a uniform hyaluronic acid. Hyaluronic acid clarification solution, spare;

[0052](2) Clean the PDMS (Polydimethylsiloxane) microneedle mold, air-dry, and set aside;

[0053] (3) Take the clear solution of hyaluronic acid in step (1), cast it onto the PDMS microneedle mold naturally, and shake it back and forth to ensure that it is evenly spread; the dosage of the above clear solution of hyaluronic acid is 0.09ml / cm 2 ;

[0054] (4) Put the PDMS microneedle mold coated with the clarified hyaluronic acid solution in a vacuum drying oven at 30°C, evacuate to 0.06MPa, hold the pressure for 2min, and slowly ...

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Abstract

The invention discloses a preparation method of autosoluble hyaluronic acid microneedles. The preparation method of the autosoluble hyaluronic acid microneedles comprises the following steps: (1) adding hyaluronic acid into purified water so as to be subjected to water-bath swelling, and allowing the swelled hyaluronic acid to stand at room temperature so as to have bubbles removed, thereby obtaining a clear hyaluronic acid solution for standby application; (2) washing a polydimethylsiloxane (PDMS) microneedle mould, and carrying out air-drying for standby application; (3) allowing natural casting of the clear hyaluronic acid solution obtained in the step (1) onto the PDMS microneedle mould, and carrying out frontward and backward oscillation so as to ensure uniform coating; and (4) putting the PDMS microneedle mould fully coated with the clear hyaluronic acid solution into a vacuum drying box, repeatedly carrying out vacuum-extraction, pressurization and exhaustion operation, and adding a desiccant so as to carry out normal-temperature drying for 24 hours until release of microneedles from the PDMS microneedle mould, thereby obtaining the autosoluble hyaluronic acid microneedles.The autosoluble hyaluronic acid microneedles prepared by the preparation method are sufficient in mechanical strength, so that, the microneedles are capable of puncturing cutaneous epidermis so as toform micron-scale channels; and thus, percutaneous transmissivity of the hyaluronic acid can be improved. Moreover, the microneedles are autosoluble after puncturing of the cutaneous epidermis.

Description

technical field [0001] The invention relates to the technical field of preparation of biological microneedles, in particular to a preparation method of self-dissolving hyaluronic acid microneedles. Background technique [0002] Hyaluronic acid is a polymer mucopolysaccharide composed of D-N-acetylglucosamine and D-glucuronic acid as structural units. Commercial hyaluronic acid is generally its sodium salt, namely Sodium Hyaluronate. Abbreviated as HA. Hyaluronic acid is a biological endogenous substance, showing a variety of physiological functions, especially, hyaluronic acid has a special water retention effect, known as an ideal natural moisturizing factor, and is widely used in medicine, biomaterials and beauty industries application. [0003] Hyaluronic acid is mainly used in the beauty industry in two ways, one is to add it to various beauty and skin care products and apply it directly, and the other is to inject it subcutaneously with the help of various syringes. ...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K47/36
CPCA61K9/0021A61K47/36
Inventor 杨明利徐群为李泽龚瑞匡义成
Owner 南京医科大学康达学院
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