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Preparation method of Gynostemma pentaphyllum extract and method for measuring content of gypenosides in Gynostemma pentaphyllum extract

A technology of Gynostemma pentaphyllum extract and determination method, which is applied in the field of traditional Chinese medicine extraction, can solve problems such as potential safety hazards, organic reagent residues, and uneven quality of Gypenosides, and achieve good precision and avoid solvent residues.

Inactive Publication Date: 2019-07-19
SCHOOL OF MEDICINE JIAYING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These extraction methods are likely to cause residues of organic reagents, resulting in greater safety hazards when people take them
The quality of gypenosides extracted from different manufacturers is uneven, which is worrying

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of embodiment 1 Gynostemma pentaphyllum extract

[0053] Take the dried whole herb of Gynostemma pentaphyllum, pass through a 50-mesh sieve after crushing, add ethanol to heat and reflux for extraction, then concentrate and dry the extract to obtain the Gynostemma pentaphylla extract; the dosage ratio of the dried Gynostemma pentaphyllum herb to ethanol is 1g : 8mL; the ethanol is a 70% aqueous ethanol solution by volume; the heating and reflux times are 3 times; each heating and reflux time is 45min.

[0054] The total saponin content of Gynostemma pentaphyllum in the Gynostemma pentaphyllum extract was determined to be 0.1025mg / ml by referring to the above method.

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Abstract

The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a preparation method of Gynostemma pentaphyllum extract and a method for measuring the contentof gypenosides in the Gynostemma pentaphyllum extract. The preparation method of the Gynostemma pentaphyllum extract comprises the following steps: taking and crushing Gynostemma pentaphyllum dried whole herb, adding ethanol, performing heating reflux extraction, and then concentrating and drying the extractive liquid to obtain the Gynostemma pentaphyllum extract. The dosage ratio of the Gynostemma pentaphyllum dried whole herb to the ethanol is 1g:(7-9)mL; the ethanol is an ethanol solution with a volume fraction of 65-75%; the heating reflux extraction number is 3-4 times; and the heating reflux time is 40-50 min. The method uses the non-toxic ethanol as an extraction solvent, and effectively avoids solvent residue caused by extraction with other toxic solvents. Meanwhile, the method can extract and obtain the gypenosides with high purity.

Description

technical field [0001] The invention relates to the technical field of extraction of traditional Chinese medicines, in particular to a preparation method of Gynostemma pentaphyllum extract and a content determination method of Gynostemma pentaphylla total saponins in the Gynostemma pentaphyllum extract. Background technique [0002] Gynostemma is a vine of the genus Gynostemma cucurbitaceae, known as "Southern Ginseng", and it is a traditional Chinese medicine that can be used as health food announced by the Ministry of Health in 2002. Its main active ingredients are gypenoside, gypenoside, water-soluble amino acids, flavonoids, multivitamins, trace elements, minerals, etc., which are beneficial to Qi and spleen, reduce phlegm and relieve cough, and clear away heat and detoxify. It has been found clinically that Gynostemma pentaphyllum has various functions such as lowering blood fat, regulating blood pressure, preventing and treating blood clots, regulating blood sugar, pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/424G01N21/31
CPCA61K36/424A61K2236/333A61K2236/39A61K2236/51G01N21/3103
Inventor 梁香钟娜娜吴凌凤程亚涛张声源丘波聂华
Owner SCHOOL OF MEDICINE JIAYING UNIV