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Preparation method and application of soya lecithin frozen diluent

A technology of soybean lecithin and freezing dilution, which is applied in the field of livestock reproduction, can solve problems such as sperm cell damage, and achieve the effects of stable properties and good cryopreservation effect.

Inactive Publication Date: 2019-07-23
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, there are many irregular large particles in the semen frozen diluent, which makes the water molecules in the solution easier to form irregular large ice crystals during the freezing process, causing irreversible mechanical damage to the sperm cells; on the other hand, soybean egg The larger particle size of phospholipids is also not conducive to its attachment to the surface of the sperm membrane to play a protective role

Method used

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  • Preparation method and application of soya lecithin frozen diluent
  • Preparation method and application of soya lecithin frozen diluent
  • Preparation method and application of soya lecithin frozen diluent

Examples

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Effect test

Embodiment 1

[0021] A preparation method of soybean lecithin frozen diluent, comprising the steps of:

[0022] (1) Add 1.8252g of citric acid, 0.5044g of glucose and 3.6342g of tris to ultrapure water for dissolution, then use a volumetric flask to set the volume to 100mL to obtain TCG solution;

[0023] (2) Add soybean lecithin at a weight percentage of 1% to the TCG solution preheated to 55°C, stir evenly, and perform ultrasonic treatment (40KHz, 50-60°C, 30min) to obtain a mixed solution;

[0024] (3) Add the mixed solution to a high-pressure homogenizer for high-pressure homogenization treatment (7°C, 2000 bar pressure, 3 times), to obtain a clear and translucent frozen dilution of soybean lecithin.

Embodiment 2

[0026] A preparation method of soybean lecithin frozen diluent, comprising the steps of:

[0027] (1) Add 1.8252g of citric acid, 0.5044g of glucose and 3.6342g of tris to ultrapure water for dissolution, then use a volumetric flask to set the volume to 100mL to obtain TCG solution;

[0028] (2) Add soybean lecithin at a weight percentage of 2% to the TCG solution preheated to 55°C, stir evenly, and perform ultrasonic treatment (40KHz, 50-60°C, 30min) to obtain a mixed solution;

[0029] (3) Add the mixed solution to a high-pressure homogenizer for high-pressure homogenization (4° C., 2000 bar pressure, 3 times), to obtain a clear and translucent frozen dilution of soybean lecithin.

Embodiment 3

[0031] A preparation method of soybean lecithin frozen diluent, comprising the steps of:

[0032] (1) Add 1.8252g of citric acid, 0.5044g of glucose and 3.6342g of tris to ultrapure water for dissolution, then use a volumetric flask to set the volume to 100mL to obtain TCG solution;

[0033] (2) Add soybean lecithin at a weight percentage of 3% to the TCG solution preheated to 55°C, stir evenly, and perform ultrasonic treatment (40KHz, 50-60°C, 30min) to obtain a mixed solution;

[0034] (3) Add the mixed solution to a high-pressure homogenizer for high-pressure homogenization treatment (4°C, 2000 bar pressure, 3 times), to obtain a clear and translucent frozen dilution of soybean lecithin.

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Abstract

The invention relates to a preparation method of a soya lecithin frozen diluent. Firstly, TCG liquid is prepared, soya lecithin is added to the preheated prepared TCG liquid in a weight percentage of1-10%, and the soya lecithin and the TCG liquid are uniformly stirred and then subjected to ultrasonic treatment to obtain a mixed solution; and finally, the mixed solution is subjected to high-pressure homogenization treatment to obtain the clear and transparent soya lecithin frozen diluent with stable properties and no precipitation after being placed for a long time. The soya lecithin frozen diluent prepared by the preparation method of the invention is used for cryopreservation of sheep semen after adding glycerol, which can improve the solubility of the soya lecithin and increase the stability of the solution by greatly reducing the particle size of the soya lecithin in the frozen diluent and has good effect on sperm cryopreservation.

Description

technical field [0001] The invention relates to a preparation method of soybean lecithin frozen diluent, which belongs to the technical field of livestock breeding. Background technique [0002] At present, the biological safety of livestock breeding has gradually attracted people's attention. Many researchers have raised objections to the addition of egg yolk, milk and other animal-derived ingredients in the semen frozen dilution, thinking that it may cause microbial contamination and have biological safety. Therefore, plant The replacement of animal-sourced active ingredients with active ingredients has become a research hotspot in current livestock sperm cryopreservation technology, and it is also a positive driving factor for the commercialization and standardized production of sperm cryodiluents. [0003] Soy lecithin (Soy lecithin) is an amphoteric surface-active phospholipid substance extracted from soybeans. It is similar to the main functional component in egg yolk ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 张树山张德福戴建军韩雪峻姜红菊吴彩凤孙玲伟吕松洁范文华
Owner SHANGHAI ACAD OF AGRI SCI
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