A kind of quality control method of spinach fruit

A quality control method and fruit technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of restricting the development and utilization of spinach fruit resources, spinach variation, lack, etc., to achieve a perfect quality control system, increase sensitivity, and good applicability Effect

Active Publication Date: 2022-03-11
GUILIN MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Spinach will mutate and mutate during the planting process, and there will be "round-leaf spinach" and "pointy-leaf spinach" in cultivation. The fruits of these two kinds of spinach are easily confused with other plant fruits. For example, the fruits of "pointy-leaf spinach" often have Thorn, very similar to the fruit of Tribulus terrestris, it is difficult to identify its authenticity based on its traits and characteristics. At present, there is a lack of fast and accurate TLC qualitative identification system for spinach fruit or dried fruit, which restricts the resource development and utilization of spinach fruit and its application as a medicinal raw material

Method used

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  • A kind of quality control method of spinach fruit
  • A kind of quality control method of spinach fruit
  • A kind of quality control method of spinach fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1) Preparation of the test solution: Take 1 g of spinach fruit powder (40 mesh), add 10 ml of methanol, weigh, ultrasonically treat for 15 minutes, take it out and let it cool, add methanol to make up for the weight loss, filter, and concentrate the filtrate to about 1.5 ml, the concentrated solution was treated at -4°C for 0.5h, filtered, and used as the test solution for later use;

[0048]2) Preparation of reference substance solution: Take an appropriate amount of β-ecdysone reference substance, add methanol to make 1ml of a solution containing β-ecdysone 0.067 mg / ml, and use it as a reference substance solution for later use.

[0049] 3) Spotting and development: According to the test of thin-layer chromatography ("Chinese Pharmacopoeia" 2015 edition four general rules 0502), draw 10 μl of the above two solutions respectively, and spot them on the same silica gel G thin-layer plate, and use chloroform-methanol (8:2) is the developing agent, and the thin-layer board...

Embodiment 2

[0053] 1) Preparation of the test solution: Take 0.5g of spinach fruit powder (10 mesh), add 5ml of methanol, weigh, ultrasonically treat for 10 minutes, take it out and let it cool, add methanol to make up for the weight loss, filter, and concentrate the filtrate to about 1.5ml, the concentrated solution was treated at -20°C for 1 hour, filtered, and used as the test solution for later use;

[0054] 2) Preparation of reference substance solution: Take an appropriate amount of β-ecdysone reference substance, add methanol to make 1ml of a solution containing β-ecdysone 0.067 mg / ml, and use it as a reference substance solution for later use.

[0055] 3) Spotting and development: According to the test of thin-layer chromatography ("Chinese Pharmacopoeia" 2015 edition four general rules 0502), respectively draw 20 μl of the above two solutions, respectively spot on the same silica gel G thin-layer plate, and use chloroform-methanol (6:1.5) is used as the developing agent, and the ...

Embodiment 3

[0059] 1) Preparation of the test solution: Take 20g of spinach fruit powder (200 mesh), add 200ml of methanol, weigh, ultrasonically treat for 60 minutes, take it out and let it cool, add methanol to make up for the weight loss, filter, and concentrate the filtrate to about 20ml , the concentrated solution was treated at -10°C for 24 hours, filtered, and used as the test solution for later use;

[0060] 2) Preparation of reference substance solution: Take an appropriate amount of β-ecdysone reference substance, add methanol to make 1ml of a solution containing β-ecdysone 0.067 mg / ml, and use it as a reference substance solution for later use.

[0061] 3) Spotting and development: According to the test of thin-layer chromatography ("Chinese Pharmacopoeia" 2015 edition four general rules 0502), draw 5 μl of the above two solutions respectively, respectively spot on the same silica gel G thin-layer plate, and use chloroform-methanol (7.5:1.9) is used as the developing agent, and...

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Abstract

The invention discloses a quality control method of spinach fruit, which belongs to the field of development and utilization of traditional Chinese medicine and medicinal and edible resources. The method uses thin-layer chromatography to identify the index component β-ecdysterone in spinach fruit or uses high-performance liquid chromatography to control β‑ecdysone, an index component in spinach fruit. The present invention adopts chemical and modern analytical techniques to establish a rapid and accurate identification of the authenticity of spinach fruits and a quality control system based on chemically active index components, breaking through the traditional method of identifying the authenticity of spinach fruits based on traits and experience only, and providing spinach Scientific, accurate, and rapid identification and detection of fruits provide technical basis. Based on the identification and detection methods, the commonly used cold filtration method is improved, and the samples are treated with low-temperature refrigeration and then filtered to reduce impurities such as proteins and lipids in the seeds. Creatively The sensitivity of the identification method is increased, the identification reliability is improved, and the entire quality control system is more perfect.

Description

technical field [0001] The invention belongs to the field of development and utilization of traditional Chinese medicine and medicinal and edible resources, and in particular relates to a quality control method of spinach fruit. Background technique [0002] Spinach fruit comes from the Chenopodiaceae spinach plant spinach ( Spinacia oleracea The fruit of L.), spinach is both edible and medicinal. Its medicinal use was first seen in "Diet Therapy Materia Medica" in the Tang Dynasty. According to "Chinese Materia Medica", spinach has the effects of stopping bleeding, nourishing blood, calming the liver, and moistening dryness , and spinach fruit (also known as spinach seed, spinach colza seed) has the effect of clearing the liver and improving eyesight, relieving cough and relieving asthma, and mainly treating wind-fire, red eyes, swelling and pain, cough and asthma. Spinach fruit contains microkeelin B, ecdysterone, α-spinasterol, stigmasterenol, stigmasterol, etc. Prelimi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/90
CPCG01N30/02G01N30/90G01N30/06
Inventor 李波刘鸿雁梁云刘渊贾忠郝明罗臻
Owner GUILIN MEDICAL UNIVERSITY
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