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Use of sabina plant polysaccharides in preparation of medicines for preventing and treating viral acute lung injury

A technology of acute lung injury and plant polysaccharides, which is applied in the preparation of drugs for the prevention and treatment of viral acute lung injury, and new drug application fields of plant polysaccharides of the genus Juniperaceae, to reduce inflammatory immune damage, reduce permeability, and inhibit replication Effect

Active Publication Date: 2019-07-26
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Throughout the research at home and abroad, there is no research report on the treatment of viral acute lung injury with polysaccharides from Juniper genus

Method used

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  • Use of sabina plant polysaccharides in preparation of medicines for preventing and treating viral acute lung injury
  • Use of sabina plant polysaccharides in preparation of medicines for preventing and treating viral acute lung injury
  • Use of sabina plant polysaccharides in preparation of medicines for preventing and treating viral acute lung injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Take 100 g of cedar cedar, crush it, extract it by cold soaking with 95% ethanol for 3 times, place the medicinal residues in a ventilated place at room temperature to dry, then extract with hot water 3 times, filter, combine the extracts, concentrate, add ethanol to the alcohol content The total polysaccharide was obtained by dialysis of the supernatant with water for 3 days, concentration of the dialysate, and freeze-drying. The sugar content of cedar polysaccharide was measured by the sulfuric acid-phenol method to be 81.2%; the uronic acid content of cedar polysaccharide was measured by the m-hydroxybiphenyl method; the protein content of cedar polysaccharide was measured by the Coomassie brilliant blue method of 1.1% %.

Embodiment 2

[0039] 36 BALB / C mice (14-16g) were randomly divided into 6 groups (N, M, A, B, C, P) according to body weight: group N was the normal group, group M was the H1N1 virus model group, and group A was the Cedar polysaccharide 7.5mg / kg, group B was cedar polysaccharide 15mg / kg, group C was cedar polysaccharide 30mg / kg, group P was positive drug ribavirin 100mg / kg, 6 animals in each group, all animals were treated with C. Poofol was anesthetized by tail vein injection, and 30 μL of 1640 culture solution was instilled in the nose in the N group as a control; the other groups were infected with 5LD via nasal infusion 50 30 μL of H1N1 virus solution was administered by intragastric administration two hours after H1N1 infection. Group N and group M were intragastrically administered with 0.5% CMC, as normal and virus controls, administered once a day for four consecutive days, H1N1 virus challenged for 96 hours After that, the body weight was weighed, the eyeball was taken out for bloo...

Embodiment 3

[0046] 60 BALB / C mice (14-16g) were randomly divided into 6 groups (N, M, A, B, C, P) according to body weight: N group was normal control group, M group was H1N1 virus model group, A group Cedar polysaccharide group 7.5mg / kg, B group cedar polysaccharide group 15mg / kg, C group cedar polysaccharide group 30mg / kg, P group ribavirin 100mg / kg, 10 in each group; all groups Animals were anesthetized with propofol, and 30 μL of 1640 culture medium in group N was used as control; 50 30 μL of H1N1 virus was administered by intragastric administration 2 hours after H1N1 infection in groups A and P, while groups N and M were intragastrically administered with 0.5% CMC, as normal and virus controls, administered once a day for seven consecutive days and then stopped. The drug was observed for 14 days, and the number of animals that survived and died was recorded every day, and the life protection rate of the drug on mice with severe influenza infection and pneumonia was calculated;

[0...

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Abstract

The invention belongs to the field of traditional Chinese medicine, and in particular, relates to a use of sabina plant polysaccharides in preparation of medicines for preventing and treating viral acute lung injury. Total polysaccharides are obtained by separating and extracting from sabina plant cedar and juniperus indica, and the sugar content is more than 60%. The integral experiment confirmsthat the polysaccharides have significant therapeutic effects on mice acute lung injury induced by influenza A virus H1N1, can inhibit the replication of influenza virus in lungs of mice, reduce the permeability of capillaries in the lungs, alleviate pulmonary hemorrhage and pulmonary edema and inhibit systemic and lung local inflammatory reactions, and significantly improve the survival rate of mice infected by severe influenza virus. The sabina plant polysaccharides can be further used for preparing the medicines for preventing and treating viral acute lung injury.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and relates to a new medicinal use of polysaccharides of the genus Juniperaceae. In particular, it relates to the use of the polysaccharide of the genus Juniper in the preparation of a medicine for preventing and treating viral acute lung injury. Background technique [0002] Studies have reported that viral acute lung injury is lung damage caused by a variety of viral infections, usually caused by the downward spread of upper respiratory viral infections. Studies have shown that influenza viruses are the most common viruses causing acute lung injury, including parainfluenza virus, cytomegalovirus, adenovirus, rhinovirus, and coronavirus. Studies have reported that influenza A virus is an important pathogen that causes respiratory diseases in humans and birds. The virus invades the bronchiolar epithelium and causes bronchiolitis, and the infection spreads to the pulmonary i...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P11/00A61P31/16A61P31/14A61P31/22A61K36/14
CPCA61K31/715A61K36/14A61K2236/331A61K2236/39A61K2236/53A61K2236/51A61K2236/333
Inventor 卢燕陈道峰傅泽龙力弘
Owner FUDAN UNIV
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