Anti-plant virus epsilon-polylysine high-yield strain and preparation process thereof

Abstract

The invention provides an anti-plant virus epsilon-polylysine high-yield strain and a preparation process. The name of the high-yield strain is Streptomyces microflavus var. liaoningen Sis X, the preservation unit is the General Microbiology Center of the Chinese Microbiological Culture Collection and Management Committee, the preservation number is CGMCC No.15355, and the preservation date is February 12, 2018; the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing City. The preparation process of anti-plant virus epsilon-polylysine comprises the following steps of strain propagation, anti-plant virus epsilon-polylysine generation by fermentation and finished product preparation process after fermentation. By adopting the anti-plant virus epsilon-polylysine high-yield strain and the preparation process, the epsilon-polylysine yield can be greatly improved, and the process has very important significance in industrial large-scale epsilon-polylysine production.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, and in particular relates to a high-yield bacterial strain resistant to plant virus ε-polylysine and a preparation process of ε-polylysine. Background technique [0002] Plant virus disease is called "plant cancer", which is the second largest plant disease after plant fungal disease, and has caused great losses to agriculture. Plant viruses have been a major problem for many crops, vegetables and ornamentals, seriously affecting the quality and yield of different crop products. The economic loss caused by plant virus diseases is as high as 60 billion U.S. dollars every year in the world, and the loss of food crops alone has reached 20 billion U.S. dollars. Once the virus invades the host, its reproduction is coupled with the metabolism of the host plant. So it is often the case that drugs that inhibit viral replication may also cause damage to the host, making it dif...

AI Technical Summary

Problems solved by technology

As a biological preservative, ε-polylysine has the characteristics of broad antibacterial spectrum and high stability. antibacterial effect, but ε-polylysine acts on the research of plant virus disease in agriculture but no report, based on this, the present invention provides Streptomyces flavinus and its antiviral ε-polylysine. Fermentation method (mainly targeting tobacco mosaic virus), which can greatly increase the yield of ε-polylysine, which provides favorable information for the long-term and even industrialized large-scale production of ε-polylysine, laying a solid foundation , and ε-polylysine is likely to become an efficient green pesticide and a regulator for promoting plant growth in the future, changing the industrial structure of pesticides and agricultural preparations in the future, and becoming a new direction for agricultural development, so its application prospects are very broad, so The present invention has very important significance