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Method for Measuring Amino Content of Chitosan Derivatives Based on Headspace Gas Chromatography

A chitosan derivative and headspace gas chromatography technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of difficulty in popularization and expensive nuclear magnetic resonance equipment, and achieves simple operation, fast measurement speed and objective results. accurate effect

Inactive Publication Date: 2021-10-26
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

Although NMR has been established as the standard for the determination of chitin deacetylation and is usually used to calibrate other methods, NMR equipment is expensive and requires professional technicians to operate, making it difficult to popularize

Method used

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  • Method for Measuring Amino Content of Chitosan Derivatives Based on Headspace Gas Chromatography
  • Method for Measuring Amino Content of Chitosan Derivatives Based on Headspace Gas Chromatography
  • Method for Measuring Amino Content of Chitosan Derivatives Based on Headspace Gas Chromatography

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Embodiment

[0037] (1) Standard sample preparation: Accurately weigh five samples of glucosamine hydrochloride (0.0100g, 0.0150g, 0.0200g, 0.0250g, 0.0300g), put them into 20ml headspace sampling vials respectively, and pour them into the headspace vials. Add 7ml of 0.01g / mL ninhydrin solution to the sample to disperse the sample evenly, then gently put it into the glass vial, carefully add 0.9mL of sodium bicarbonate solution (concentration 1.2mol / L) with a pipette gun, and seal it with the bottle cap ; put the whole headspace vial in a water bath at 90°C for 15 minutes, shake the vial manually to mix the sodium bicarbonate in the glass vial with the ninhydrin solution containing chitosan derivatives, and then place it at 60 ℃ water bath reaction for 60 minutes; also prepare a control blank bottle (does not contain glucosamine hydrochloride), and the others are exactly the same as above. After the reaction, it can be clearly found that purple substances were formed in the headspace vials...

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Abstract

The invention discloses a method for measuring the amino group content of chitosan derivatives based on headspace gas chromatography. The method is to use excess ninhydrin to react with an amino group-containing standard substance, and then use sodium bicarbonate to react with the remaining ninhydrin The reaction generates carbon dioxide. No standard substance is added to the control group. The peak area of ​​carbon dioxide is measured by headspace gas chromatography. The relative reduction of carbon dioxide is linear in relation to the quality of the standard substance. A standard curve is established, and then the standard substance is replaced by the standard substance to be tested. Chitosan derivatives with amino content, and detect the peak area of ​​its carbon dioxide, obtain the quality of the corresponding standard substance according to the calibration curve, and calculate the amino content at this time. Compared with the traditional conductometric titration method, the relative error is no more than 13.03%, and for the detection of the same sample, the relative standard deviation is at most 4.83%, so the result is objective and accurate, and faster than the traditional determination method.

Description

technical field [0001] The invention relates to the technical field of determining the amino content of chitosan derivatives, in particular to a method for measuring the amino content of chitosan derivatives based on headspace gas chromatography. Background technique [0002] Headspace gas chromatography, also known as headspace gas chromatography. Headspace gas chromatography uses the principle that after the volatile components in the liquid (solid) body reach equilibrium in a closed constant temperature system, the ratio of the volatile components in the gas phase and the liquid (solid) phase is constant, and the vapor in the upper part of the liquid (solid) after equilibrium is analyzed. Perform gas chromatographic analysis. It can also carry out targeted chemical reactions during the sample balance process, and convert the components (non-volatile) that cannot be determined by gas chromatography into volatile components, so as to realize the detection of the non-volati...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/16
CPCG01N30/02G01N30/16
Inventor 万小芳刘梦珍陈广学柴欣生
Owner SOUTH CHINA UNIV OF TECH
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