Quality detection method of Mongolian medicine red sandalwood heart based on hplc fingerprint

A quality detection method and fingerprint technology, which are applied in the field of quality detection of red sandalwood heart medicinal materials, can solve problems such as long calculation time, prolong quality detection period, unfavorable actual quality control, etc., and achieve high detection sensitivity, fast calculation speed, and detection short cycle effect

Inactive Publication Date: 2021-08-06
INNER MONGOLIAN AUTONOMOUS REGION INST OF CHINESE MEDICINE
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  • Abstract
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Problems solved by technology

[0003] At present, the quality detection of red sandalwood heart medicinal materials is usually carried out by infrared spectroscopy, but because the infrared spectral data obtained by this method is a spectrogram, it cannot be used in the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2004A Edition, compiled by the National Pharmacopoeia Committee"). )” software for data processing to calculate the similarity, can only copy the source file data of infrared spectrograms of all red sandalwood samples from the workstation of BRUKER IFS-55 Fourier infrared spectrometer, transfer them to TXT files, and then import them into word, Transfer to Excel to calculate the similarity through a specific calculation formula; therefore, it takes a long calculation time, which prolongs the quality inspection cycle and is not conducive to actual quality control

Method used

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  • Quality detection method of Mongolian medicine red sandalwood heart based on hplc fingerprint
  • Quality detection method of Mongolian medicine red sandalwood heart based on hplc fingerprint
  • Quality detection method of Mongolian medicine red sandalwood heart based on hplc fingerprint

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Experimental program
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Effect test

Embodiment 1

[0035] Instruments and materials:

[0036] Therm3000 high performance liquid chromatography, Thermo Fisher automatic sampler, diode array detector, ultrasonic cleaner: model, KQ3200DV numerical control, Kunshan Ultrasonic Instrument Co., Ltd.; universal high-speed pulverizer, model: FLB-500A, Shanghai Fei Libo Food Machinery Co., Ltd.; ultra-pure water machine, model: XJG-40-B, Shenyang Xinjie Technology Co., Ltd.; electric blast drying oven (Shanghai-Heng Scientific Instrument Co., Ltd.); AL104 electronic balance (Meite Le Toledo Instrument Co., Ltd.), column Thermo C18 (250×4.6mm5μm); red sandalwood medicinal materials (batch numbers: 20100829, 20100830, 20120920, 1203011), provided by the National Mongolian Medicine Preparation Center; (batch numbers: 20100203, 100501, 20140513 ), purchased from different local pharmacies; Indian red sandalwood heartwood (batch numbers: 20150618, 20150714), purchased from the Fozhu Market in Hohhot; the control substance was genuine Mongoli...

Embodiment 2

[0044] 1. See Table 1 for the sources of samples of red sandalwood medicinal materials used in material experiments.

[0045] Table 1 Mongolian medicine red sandalwood HPLC fingerprint sample batch list

[0046] Red sandalwood medicinal material number Sample serial number batch number source 1 S1 9814104 Provided by the Mongolian Medicine Department of the Inner Mongolia Institute for Food and Drug Control 2 S2 20100203 Bought from Furui Pharmacy Store in Hohhot Origin: Unknown 3 S3 100501 Purchased from Hexiantang Dayao, Hohhot. Real estate location: Hainan 4 S4 20100829 Bought from Shouchuntang Pharmacy in Hohhot Origin: Unknown 5 S5 20100830 National Mongolian Medicine Preparation Center provides origin: Guangdong 6 S6 1203011 National Mongolian Medicine Preparation Center provides origin: Guangdong 7 S7 20140513 Bought from Shouchuntang Pharmacy in Hohhot Origin: Guangdong 8 S8 20150806 Indian r...

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Abstract

The invention discloses a method for detecting the quality of Mongolian medicine red sandalwood heart medicinal material based on HPLC fingerprints, which comprises the following steps: step (1): preparation of the test solution; step (2): preparing the Need testing solution injects in the high performance liquid chromatograph and detects, obtains need testing chromatogram; Step (3): the preparation of contrast solution; Step (4): inject the contrast solution prepared in step (3) into high performance liquid Detect in the chromatograph, obtain contrast fingerprint; Step (5): compare the contrast fingerprint obtained in step (2) with the contrast fingerprint obtained in step (4), and calculate the relative standard deviation RSD% . The invention can be used as a means for authenticity identification and quality inspection of the red sandalwood medicine, and has the advantages of safety, reliability and quality control.

Description

Technical field: [0001] The invention relates to a quality detection method of red sandalwood medicinal material, in particular to a quality detection method of Mongolian medicine red sandalwood medicinal material based on HPLC fingerprint. Background technique: [0002] As a Mongolian medicinal material, red sandalwood is a high-frequency clinical medicine in Mongolian medicine. Red sandalwood is the powder of the dried heartwood of the butterfly-shaped plant Pterocarpus indicus Willd. It is native to India and Southeast Asia, and it is also cultivated in Yunnan, Hainan and other places in my country; Alias: red sandalwood, rose wood, green dragon wood, red blood tree, etc. Its heartwood is used as the part of medicine, which has the effects of clearing blood heat, regulating "Heyi" blood stasis, and reducing swelling. Clinically, it is mainly used for blood heat, blood stasis and tingling pain, "Heyi" blood conflict, "Baori" fever, liver fire, heart heat, lumps and other d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86
CPCG01N30/8686G01N30/8696
Inventor 朱明张红霞王静
Owner INNER MONGOLIAN AUTONOMOUS REGION INST OF CHINESE MEDICINE
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