Multiple gene detection kit used for medication guidance of anti-manic medicine and use method thereof
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A technology for medication guidance and genetic testing, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of long time, high cumulative price, high cost, avoid false negatives and false positives, and achieve good repeatability. , a strong specific effect
Pending Publication Date: 2019-08-02
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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Problems solved by technology
However, the Sanger sequencing method is complex and expensive
When there are many sequencing sites, it needs to be sequenced one by one, which takes a long time and the cumulative price is relatively expensive
The next-generation sequencing technology realizes sequencing while synthesizing, which has the advantages of high throughput and high efficiency. However, the next-generation sequencing platform is expensive and has low popularity, and its clinical application as a SNP detection technology is not mature enough.
[0014] Due to the large number of gene SNPs related to the use of antimanic drugs, the above techniques have obvious limitations, so it is difficult to apply multiple gene detection for the guidance of antimanic drug use
[0015] At present, there is no report on the multiplex gene detection scheme based on multiplex PCR and CE separation technology for the guidance of antimanic drug medication in China
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Embodiment 1
[0041] In this example, the oral exfoliated cells of a manic patient were collected, multiple PCR reactions were directly performed using the cell collection card as a template, and finally the samples were separated by capillary electrophoresis. The specific steps are as follows:
[0042] 1. Production of multiple gene detection kits for antimanic drug medication guidance, including the following components:
[0043] 1) Primer composition Mania Primer Mix;
[0044] 2) PCR reaction solution;
[0045] 3) Positive control substance Mania POS;
[0046] 4) Ultrapure water.
[0047] 2. Collection of samples
[0048] The oral exfoliated cells of a manic patient were collected with a buccal swab and stored on a cell collection card.
[0049] 3. Use the cell collection card as a template for PCR reaction
[0050]1) Add reagents and samples to the 96-well sample plate / PCR eight-tube tube according to Table 4.
[0051] Table 4 PCR reaction system
[0052] Reagent Amou...
Embodiment 2
[0072] In this embodiment, a whole blood sample of a manic patient is collected and nucleic acid is extracted, multiple PCR reactions are carried out using the extracted nucleic acid as a template, and finally the sample is separated by capillary electrophoresis. The specific steps are as follows:
[0073] 1. Production of multiple gene detection kits for antimanic drug medication guidance, kit components as described in Example 1.
[0074] 2. Collection of samples
[0075] A whole blood sample was taken from a manic patient and nucleic acid was extracted.
[0076] 3. Use the extracted nucleic acid as a template for PCR reaction
[0077] Add reagents and samples to the 96-well sample plate / PCR eight-tube tube according to Table 9.
[0078] Table 9 PCR reaction system
[0079] Reagent Amount (μL) / well PCR reaction solution 14 Primer composition 2 nucleic acid 2 water 2 Total 20
[0080] 2) Mix and centrifuge the prepared PCR sys...
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Abstract
The invention discloses a multiple gene detection kit used for medication guidance of an anti-manic medicine and a use method thereof. According to the multiple gene detection kit used for medicationguidance of the anti-manic medicine and the use method thereof, multiple PCR in combination with a fragment length/quality analysis method is adopted, so that two single nucleotide polymorphism (SNP)sites on GSK3B and NTRK2 genes which are related to medication of the anti-manic medicine are detected synchronously, fast and qualitatively. The use method of the multiple gene detection kit used formedication guidance of the anti-manic medicine comprises the detection steps of (1) collecting oral cavity exfoliative cells, and storing the oral cavity exfoliative cells in a cell collection card or collecting a blood sample, and extracting nucleic acid; (2) adopting the cell collection card or the nucleic acid in the step (1) as a template for multiple PCR amplification; (3) according to PCR product fragment length/quality, separating the two SNP sites, three human genome DNA reference genes and a PCR reaction reference PCR product synchronously; and (4) analyzing and judging results. Themultiple gene detection kit used for medication guidance of the anti-manic medicine has the advantages of being fast, high in sensibility, good in repeatability, high in specificity, high in flux andlow in cost.
Description
technical field [0001] The invention relates to a multiple gene detection kit, in particular to a multiple gene detection kit for guiding the administration of antimanic drugs and a method for using the same. Background technique [0002] Mania is an affective mental disorder. The basic characteristics of the disease are that the patient's thoughts are running wild, the mood is high, and the speech and actions are more than usual. The main clinical manifestations are irritability and high mood. Mania is often accompanied by depressive episodes, so mania has been listed as a type of bipolar disorder. Most patients with mania will have recurrent episodes, and severe patients will also be accompanied by psychotic symptoms such as hallucinations and delusions. [0003] Medication is the mainstay of treatment for mania. As the pathogenesis of mania is gradually revealed, the development of antimania drugs has also achieved rapid development. Clinically, mood stabilizers are of...
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