Neuroglioma 1p/19q co-deletion detection system based on next generation sequencing

Abstract

The invention discloses a neuroglioma 1p / 19q co-deletion detection system based on next generation sequencing. The system comprises an SNP locus screening device, an SNP detection device without a check sample and / or an SNP detection device with a check sample, wherein the SNP locus screening device is used for screening SNP loci on the first chromosome and nineteenth chromosome of the human according to an existing database to obtain a first group of SNP loci, and the SNP detection device without the check sample comprises a first sequencing module used for sequencing a to-be-sequenced sampleand a group of negative samples, a first SNP detection module, a first gSNP locus screening module, a second SNP detection module, a first calculating statistics module and a first judgement module.The system is utilized and combined with information on 1q and 19 p to correct information of 1p and the 19q, the detection accuracy is improved, and 1p / 19q co-deletion can be identified efficiently,conveniently and accurately.

Description

technical field [0001] The present invention relates to the technical field of medical devices, in particular to a system for detecting combined deletion of 1p / 19q in glioma based on next-generation sequencing. Background technique [0002] High-Throughput Sequencing technology (High-Throughput Sequencing) is to connect DNA adapters to prepare a sequencing library, perform extension reactions on tens of thousands of clones in the library, detect corresponding signals, and finally obtain sequence information. Sequencing up to millions of DNA molecules is a revolutionary change to traditional generation sequencing, so it is called Next Generation Sequencing (NGS). At the same time, high-throughput sequencing enables the transcriptome and genome of a species to be Detailed and comprehensive analysis becomes possible, so it is also called deep sequencing. [0003] The NGS detection method has high throughput and can detect a large number of genes to meet the needs of clinical d...

AI Technical Summary

Problems solved by technology

[0006] (2) 454 sequencing technology (long read length, but low accuracy, high cost, and pyrosequencing technology, the duration accounts for

[0014] 2) High-grade gliomas (WHO grade III-IV) are poorly differentiated gliomas; these tumors are malignant tumors, and the prognosis of patients is poor

Detect the lack of judgment of a small number of fixed STR intervals on 1p and 19q, and the judgment range is not as large as that of NGS, and if homozygosity occurs in the STR interval, it cannot be included in the judgment result, reducing the accuracy of the result

And the operation is complicated, and the results are mostly based on the subjective judgment of the experimenter, so the results cannot be judged conveniently and accurately

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