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Method of simultaneously evaluating oxidation resistance active constituents and contents in downy rosemyrtle root

A technology of myrtle root and content, which is applied in the field of analysis of antioxidative active components of Chinese herbal medicines, can solve the problems of high reagent consumption, long time consumption, and expensive instruments, and achieve the effects of high sensitivity, low reagent consumption, and easy operation

Inactive Publication Date: 2019-08-09
GUANGXI VETERINARY RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The UV method is cumbersome, consumes a lot of reagents, takes a long time, and has poor reproducibility; the HPLC-UV-DPPH method takes a long time; the HPLC-DPPH-UV-MS method is expensive, has high requirements for operators, and has a low penetration rate

Method used

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  • Method of simultaneously evaluating oxidation resistance active constituents and contents in downy rosemyrtle root
  • Method of simultaneously evaluating oxidation resistance active constituents and contents in downy rosemyrtle root
  • Method of simultaneously evaluating oxidation resistance active constituents and contents in downy rosemyrtle root

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Experimental program
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Effect test

Embodiment 1

[0020] (1) Configuration of standard solution: Accurately weigh appropriate amounts of gallic acid reference substance and ellagic acid reference substance respectively, place them in a 50mL brown volumetric flask, add methanol to dissolve and dilute to the mark, and prepare 98.72μg / mL gallic acid and tannic acid A mixed reference solution of 74.01 μg / mL flower acid; before use, dilute the above standard solution with methanol in order to prepare standard working solutions with different concentrations;

[0021] (2) Sample pretreatment: powder the dried myrtle root from Jinxiu, Guangxi, and sieve it to obtain the sample powder of the myrtle root medicinal material to be tested, and store it in a desiccator; accurately weigh 0.5 g of the fine powder and place Add 50mL of methanol to a 100mL Erlenmeyer flask with a stopper, weigh it, extract it by ultrasonic (350W, 53kHz, 35°C) for 40min, let it cool, filter, evaporate to dryness, dissolve the residue with methanol, and dilute to...

Embodiment 2

[0027] (1) The configuration of the standard solution is the same as in Example 1.

[0028] (2) Sample pretreatment: powder the dried myrtle root from Yulin, Guangxi, and sieve it to obtain the sample powder of the myrtle root medicinal material to be tested, and store it in a desiccator; accurately weigh 0.5g of the fine powder and place it in the Add 50mL of methanol to a 100mL Erlenmeyer flask with a stopper, weigh it, extract it ultrasonically (350W, 53kHz, 40°C) for 30min, let it cool, filter, evaporate to dryness, dissolve the residue with methanol, and dilute to a 25mL volumetric flask. Accurately draw 5mL of the above solution and place them in a 10mL brown volumetric flask, respectively add methanol and 5.04mmol / L DPPH solution to the volume, shake well, and react in the dark for 30min at room temperature to obtain the test samples before and after DPPH reaction The solution was measured after passing through a 0.2 μm filter membrane.

[0029] (3) The chromatographic...

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Abstract

The invention relates to a method of simultaneously evaluating the oxidation resistance and the contents of the active constituents of gallic acid and ellagic acid in a downy rosemyrtle root by usingDPPH-UPLC. The method comprises the following steps: a to-be-detected sample before and after reaction with the DPPH is subjected to ultra-high performance liquid chromatography detection; and based on gallic acid and ellagic acid peak areas of a test solution before and after DPPH reaction detected by UPLC, gallic acid and ellagic acid contents and clearance rates are calculated. The oxidation resistance and the contents of the active constituents in the downy rosemyrtle root can be detected at the same time, the operation is simple, quick and convenient, the consumption of a reagent is little, the sensitivity is high, the oxidation resistance active constituents and the contents in the downy rosemyrtle root can be evaluated, and a theoretical basis is provided for screening and further development of the downy rosemyrtle oxidation resistance active constituents.

Description

technical field [0001] The invention relates to the field of analysis of antioxidative active components of traditional Chinese medicinal materials, in particular to a method for simultaneously evaluating the antioxidative properties and contents of gallic acid and ellagic acid, effective components in myrtle root, by using DPPH-UPLC. Background technique [0002] The main active ingredients in myrtle root are gallic acid and ellagic acid, and gallic acid and ellagic acid have been proven to have antioxidant effects. Therefore, evaluating the antioxidant active ingredients and their content in myrtle root medicinal materials can Provide a theoretical basis for the screening and further development of myrtle antioxidant active ingredients. [0003] At present, the methods for evaluating the antioxidant properties of active ingredients in Chinese herbal medicines are mainly UV method, HPLC-UV-DPPH method, HPLC-DPPH-UV-MS method, etc. The UV method is cumbersome, consumes a lo...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 银慧慧刘伟赵武孟菲姜源明覃振华孙建华
Owner GUANGXI VETERINARY RES INST
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