Method for extracting lycorine

Abstract

The invention provides a method for extracting lycorine. The method comprises the following steps: (1) soaking lycoris radiate bulb powder with water, then adding a compound enzyme for hydrolysis, carrying out filtering, and merging the filtrate; and (2), adding acidic clay into the filtrate obtained in the step (1), carrying out stirring, and the carrying out filtering; (3) adjusting the pH valueof the filtrate obtained in the step (2) by using a saturated sodium carbonate solution, carrying out filtering after standing, extracting the filter cake obtained by filtering, merging the filtrate,and concentrating the filtrate into paste to obtain a lycorine crude product; and (4) carrying out reflux and crystallization on the lycorine crude product obtained in the step (3) to obtain a lycorine pure product. The method disclosed by the invention has the advantages that the purity of the lycorine extracted from the plant lycoris radiate is greater than or equal to 99%, the product yield isincreased, the solvent loss is less than or equal to 2%, and the lycorine crude product which is high in purity and can be used for anti-tumor lycorine products is obtained, so that the yield is improved to the maximum extent, and a good technical support is created for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, in particular to a method for extracting lycorine. Background technique [0002] Lycoris belongs to the genus Lycoris, with bulbs that are nearly spherical. Leaves emerge in autumn, narrowly banded, blunt at the top, dark green with a pink-green band in the middle. Lycorine is an important component of Lycoris with physiological and pharmacological activities, and has extremely high medicinal value, especially high-purity lycorine has a strong anti-tumor effect, as well as antibacterial and cardiovascular protection effects. It can be used to develop drugs for treating major diseases such as cancer and cardiovascular disease. At present, the extraction technology of lycorine is not mature enough. Most of the extracted lycorine is a mixture of various alkaloids. Since the efficacy of different alkaloids varies greatly, it is difficult to obtain such a mixture of alkaloids. If it is dir...

AI Technical Summary

Problems solved by technology

At present, the extraction technology of lycorine is not mature enough. Most of the extracted lycorine is a mixture of various alkaloids. Since the efficacy of different alkaloids varies greatly, it is difficult to obtain such a mixture of alkaloids. If it is directly used, it will seriously affect the efficacy of the drug, and it must be separated and purified first; and the separation and purification of these alkaloids is quite complicated, and has many defects such as large consumption of organic solvents, serious environmental pollution, and long operating hours. It directly brings problems such as environmental protection and high product cost. However, when extracting lycorine from lycoris in the prior art, it is difficult to make lycorine reach a purity of 95%, which cannot meet the requirements for the treatment of intractable diseases.

At the same time, the yield of the existing technology is <0.01‰, and the solvent loss is ≥15%, which makes it difficult to form large-scale production

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