Application of total alkaloids in melodinus khasianus to preparation of medicine for preventing and treating inflammatory bowel disease

A technology for inflammatory bowel disease and total alkaloids, applied in the field of medicine, can solve the problems of prevention and treatment of inflammatory bowel disease, such as no patent literature reports, and achieve the effect of market demand, promising prospects, wide sources, and low cost

Active Publication Date: 2019-08-20
云南聚和生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Jingdongshan orange is rich in indole alkaloids, terpenoids, sterols and volatile oils, which have a good regu

Method used

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  • Application of total alkaloids in melodinus khasianus to preparation of medicine for preventing and treating inflammatory bowel disease
  • Application of total alkaloids in melodinus khasianus to preparation of medicine for preventing and treating inflammatory bowel disease
  • Application of total alkaloids in melodinus khasianus to preparation of medicine for preventing and treating inflammatory bowel disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Extraction and Preparation of Jingdongshan Orange Total Alkaloids

[0023] Take by weighing 100g of pulverized Jingdongshan orange branches and leaves, according to the ratio of solid-liquid ratio g:mL=1:10, use methanol aqueous solution with a volume concentration of 90% for ultrasonic extraction for 30min, filter, and after repeated ultrasonic extraction of filter residue for 3 times, combine the filtrates, The extract was obtained after evaporation and concentration at 45°C; the extract was dissolved in aqueous hydrochloric acid solution with a volume concentration of 0.3%, adjusted to pH 2-3, extracted with ethyl acetate, the water layer was collected, and then ammonia water with a volume concentration of 5% was added. Adjust the pH to 9-10, then extract with ethyl acetate, collect the ethyl acetate layer, evaporate and concentrate, freeze-dry to obtain the total alkaloids of Jingdongshan orange.

Embodiment 2

[0024] Embodiment 2: Apply the method of DSS induction to establish the model of UC, observe the preventive effect of total alkaloids of Jingdongshan orange on UC through animal model experiments

[0025] 1. Establishment of UC model and experimental process

[0026] Male C57BL / 6 mice (6-8 weeks, weight 21-23g) were used as research objects, with 8 mice in each group; the extreme concentration was established by administration of 2.5% DSS (dextran sodium sulfate) for 7 consecutive days 5-aminosalicylic acid (SASP) was used as a positive drug, and the total alkaloids of Jingdongshan orange were administered to evaluate its preventive effect on UC; the mice were adapted to the standard environment for seven days after purchase, and the standard Feed for feeding. The specific experimental procedures and groups are as follows:

[0027] Negative control group (Control): normal drinking water

[0028] Model group (Model): administrated with normal saline for 10 consecutive days, ...

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Abstract

The invention discloses new application of total alkaloids in melodinus khasianus, that is, application of a medicine for preventing or/and treating an inflammatory bowel disease, and belongs to the technical field of development of natural drugs or medicines. According to the application of the total alkaloids in the melodinus khasianus disclosed by the invention, by using aerial parts of the melodinus khasianus as raw materials, the total alkaloids in the melodinus khasianus are obtained through ultrasonically extracting methanol water and performing acid-alkali treatment; the plants of themelodinus khasianus are widely distributed and are low in cost, and raw materials are easy to get, and therefore, an extraction process of the alkaloids is simple. According to the application of thetotal alkaloids in the melodinus khasianus disclosed by the invention, a colonitis model is built through DSS-induced C57BL/6 mice, and by adopting the total alkaloids in the melodinus khasianus, expression of related inflammatory factors in DSS-induced colon tissues can be effectively inhibited, the activity of related oxydase in the colon tissues can be improved, and the total alkaloids in the melodinus khasianus have certain prevention and treatment effects on the inflammatory bowel disease and have good development and application prospects in preparation of the medicine for preventing or/and treating the inflammatory bowel disease.

Description

technical field [0001] The invention relates to the application of total alkaloids of Jingdongshan orange in the preparation of medicines for preventing and treating inflammatory bowel disease, and belongs to the technical field of medicines. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a non-specific intestinal disease characterized by chronic inflammation and ulceration. At present, there is no effective cure. It is a chronic disease of the digestive system. , weight loss, purulent mucus stool, and tenesmus are the main symptoms; the lesion of the disease is mainly the mucosa and submucosa of the colon, mainly ulcers and edema, mostly involving the rectum, distal colon or even the entire colon. It is the result of multi-factors, multi-mechanisms, and multi-links. The course of the disease is long, the disease is prone to relapse and delay in healing, and it can cause serious complications and the possibility of cancer. It ha...

Claims

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Application Information

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IPC IPC(8): A61K36/24A61P1/00
CPCA61K36/24A61K2236/333A61K2236/55A61P1/00
Inventor 程桂广杨美莲何舒悦曹建新赵天瑞
Owner 云南聚和生物科技有限公司
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