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Immune mouse model with pulmonary delivery of liquid aerosol of Yersinia pestis F1 vaccine

An aerosol and vaccine technology, applied in the direction of bacterial antigen components, antibody medical components, preparations for in vivo tests, etc., can solve the problems of large sample loss, large equipment volume, high price, etc., to achieve good protection effect, Reproducible and targeted effects

Inactive Publication Date: 2019-08-20
ACADEMY OF MILITARY MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most studies on liquid aerosol or dry powder aerosol use oral and nasal exposure and whole body exposure. Although these two methods are non-invasive to experimental animals, they cannot accurately quantify the dosage, and the samples are lost in the exposure tower and exposure chamber. Huge, unsuitable for inhalation administration of expensive, tiny samples such as protein vaccines
Moreover, the related instruments and equipment are bulky and expensive.

Method used

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  • Immune mouse model with pulmonary delivery of liquid aerosol of Yersinia pestis F1 vaccine
  • Immune mouse model with pulmonary delivery of liquid aerosol of Yersinia pestis F1 vaccine
  • Immune mouse model with pulmonary delivery of liquid aerosol of Yersinia pestis F1 vaccine

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Embodiment 1

[0042] Example 1. A method for preparing immune mouse model by liquid aerosol delivery to lung

[0043] 1. Cultivation of Yersinia pestis and preparation of its generating liquid

[0044] (1) Activation: Thaw the glycerin strains stored in the refrigerator at -80 degrees and inoculate 20 μl into 20 ml of BHI broth, and cultivate them on a shaker at 26° C. and 200 rpm for 36 hours to make Y. pestis grow to a plateau. This is the first generation of bacteria.

[0045] (2) Pre-cultivation: Dilute the bacterial solution 20 times and transfer it to 20ml of BHI broth, and incubate the bacterial solution to OD on a 26℃ shaker at 200 rpm 600 Is 1.0. This is the second generation of bacteria.

[0046] (3) Formal culture: Dilute the bacterial solution 100 times and transfer it to 20ml of BHI broth, and incubate the bacterial solution to OD at 200 rpm at 26°C 600 1.0, this is the third generation of bacteria. The third-generation bacterial solution was transferred to a 37°C shaker at 200 rpm f...

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Abstract

The invention discloses an immune mouse model with pulmonary delivery of liquid aerosol of a Yersinia pestis F1 vaccine. The invention provides a method for preparing an animal model. The method for preparing the animal model comprises the following steps: delivering immunogen to lungs of animals by the means of liquid aerosols; and then, carrying out challenging by using pathogen corresponding tothe immunogen so as to obtain the animal model. According to the method for preparing the animal model, tested substances are directly delivered to lungs of mice by the means of liquid aerosols so asto construct a novel immune mouse model; and the constructed model is highly targeted, good in repeatability, and capable of allowing accurate and quantitative study of immune status of the tested substances in mice.

Description

Technical field [0001] The invention relates to a preparation method of a mouse inhalation immunity model, in particular to a mouse model of Y. pestis F1 vaccine liquid aerosol lung delivery immunity. Background technique [0002] Yersinia pestis is a gram-negative bacterium belonging to the Yersinia enterobacteriaceae, which can cause plague outbreaks. Plague is a natural epidemic disease that spreads among rodents and is spread by fleas. It has caused three worldwide pandemics in history, claiming hundreds of millions of lives. Yersinia pestis is one of the bacteria in the genus Yersinia pestis that can cause serious human infections. It can cause three main syndromes, manifesting pneumonic plague, sepsis or bubonic plague. Pneumonic plague occurs through aerosol droplets. Spread; the main cause of sepsis is the bite of an infected fleas. If no treatment is taken, the fatality rate can reach 100%; bubonic plague is also spread by fleas, and no treatment can lead to a fatality...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K49/00A61D7/00
CPCA61K39/0208A61K49/0008A61D7/00Y02A50/30
Inventor 冯俊霞杨文慧周冬生孙岩松邱业峰高波法云智王效义杨慧盈殷喆胡凌飞焦俊靳爱军熊小路赵月娥于学东焦周光
Owner ACADEMY OF MILITARY MEDICAL SCI
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