Steady-state malvidin 3-O-glucoside derivative and preparation method and applications thereof

A technology of glycoside derivatives and glucoside, which is applied in the field of stable mallow 3-O-glucoside derivatives and its preparation, can solve problems such as inability to meet production needs, low efficiency, and no reports, and achieve good light Effects of thermostability and anti-oxidative stress

Active Publication Date: 2019-08-20
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this spontaneous steady-state reaction is slow and inefficient, which cannot meet the production needs
Due to the high pressure, ultra-high pressure has the advantages of instantaneous compression, uniform action, safe operation, and eco-friendliness, and can extract substances to the greatest extent; however, there are few related studies on the use of ultra-high pressure technology to promote pigment stability, and There is no report about the high-pressure stabilization treatment of mallow 3-O-glucoside and caffeic acid and its mechanism; after ultra-high pressure stabilization treatment, mallow 3-O-glucoside is derived into its derivatives, and this The derivative is stable in nature, and its antioxidant effect is better than that of mallow 3-O-glucoside, which can be widely used in the development of anti-oxidative stress products

Method used

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  • Steady-state malvidin 3-O-glucoside derivative and preparation method and applications thereof
  • Steady-state malvidin 3-O-glucoside derivative and preparation method and applications thereof
  • Steady-state malvidin 3-O-glucoside derivative and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Wild grape pretreatment: After the wild grape is screened, removed, and cleaned, use a colloid mill at 2000r / min to grind for 20min to obtain a wild grape slurry for subsequent use;

[0044] (2) Extraction: 0.1% hydrochloric acid-75% ethanol (v / v) according to the liquid-to-material ratio of 5:1 (w / w), that is, 100mL solution contains 0.1mL of HCl and 75mL of ethanol, and the rest is deionized water ) was added to the grape grinding slurry, extracted by high-voltage pulsed electric field, the electric field strength was 10kV / cm, and the number of pulses was 10, to obtain anthocyanin extract;

[0045] (3) Centrifugal concentration: centrifuge the above anthocyanin extract at 3000r / min for 15min, discard the filter residue, take the supernatant, concentrate under reduced pressure at 45°C, 40r / min, and vacuum 0.08Mpa to a density of 1.0× 10 3 kg / m 3 , to obtain anthocyanin concentrate;

[0046] (4) Purification of D101 macroporous resin: dilute the concentrated anth...

Embodiment 2

[0053] (1) Wild grape pretreatment: After the wild grape is screened, removed, and cleaned, use a colloid mill at 4000r / min to grind for 10 minutes to obtain the wild grape slurry, which is set aside;

[0054] (2) Extraction: 0.5% hydrochloric acid-55% ethanol (v / v, i.e. 100mL solution contains 0.5mL of HCl and 55mL of ethanol, the rest being deionized water) extract was added to the grape grinding slurry, and extracted by high-voltage pulsed electric field, the electric field strength was 20kV / cm, and the number of pulses was 4 to obtain anthocyanin extract;

[0055] (3) Centrifugal concentration: centrifuge the above anthocyanin extract at 5000r / min for 5min, discard the filter residue, take the supernatant, concentrate under reduced pressure at 55°C, 60r / min, and vacuum 0.1Mpa to a density of 1.1× 10 3 kg / m 3 , to obtain anthocyanin concentrate;

[0056] (4) Purification of D101 macroporous resin: Dilute the concentrated anthocyanin solution 10 times with distilled water...

Embodiment 3

[0063] (1) Wild grape pretreatment: After the wild grape is screened, removed, and cleaned, use a colloid mill 3500r / min to grind for 15 minutes to obtain the wild grape slurry, which is set aside;

[0064] (2) Extraction: 0.1% hydrochloric acid-65% ethanol (v / v, i.e. 100mL solution contains 0.1mL of HCl and 65mL of ethanol, the rest being deionized water) extract was added to the grape grinding slurry, and extracted by high-voltage pulsed electric field, the electric field strength was 15kV / cm, and the number of pulses was 6 to obtain anthocyanin extract;

[0065](3) Centrifugal concentration: centrifuge the above anthocyanin extract at 4500r / min for 10min, discard the filter residue, take the supernatant, concentrate under reduced pressure at 50°C, 50r / min, and vacuum 0.09Mpa to a density of 1.05× 10 3 kg / m 3 , to obtain anthocyanin concentrate;

[0066] (4) Purification of D101 macroporous resin: dilute the anthocyanin concentrate 5 times with deionized water, and load t...

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Abstract

The invention discloses a steady-state malvidin 3-O-glucoside derivative and a preparation method and applications thereof. The method comprises the following steps: subjecting amur grapes to pulping,extraction, macroporous resin purification and preparative high performance liquid chromatography purification to obtain malvidin 3-O-glucoside, and carrying out ultrahigh-pressure steady-state treatment on the malvidin 3-O-glucoside and caffeic acid to obtain the steady-state malvidin 3-O-glucoside derivative. The method has the beneficial effects that: the anthocyanin is protected from being damaged by high-voltage pulse electric field extraction; the purification condition is optimized, and the purity of the prepared malvidin 3-O-glucoside reaches 95 percent; and the malvidin 3-O-glucosideand the caffeic acid are subjected to the ultrahigh-pressure steady-state treatment, so that the derivative has better photo-thermal stability and oxidation resistance.

Description

technical field [0001] The invention relates to the technical field of anthocyanin stabilization, and more specifically relates to a stabilized mallow 3-O-glucoside derivative and its preparation method and application. Background technique [0002] Anthocyanins are water-soluble flavonoids with a basic structure of 3,5,7-trihydroxy-2-phenylbenzopyran, which are widely found in natural plants, especially in grapes, lingonberries, and mulberries. Rich. Mountain grapes have been planted in Ji'an, Liuhe and other places in Northeast my country for more than 50 years. The cultivation area is large and the annual output is high. The content of anthocyanins in mountain grapes in Northeast China is rich, and individual varieties are as high as 400mg / (100g.FW). The content of anthocyanins is the highest, accounting for more than 55% of the total anthocyanins, which can be used as a good source of natural anthocyanins. Natural anthocyanins have a good antioxidant effect and have gre...

Claims

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Application Information

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IPC IPC(8): C07H1/00C07H1/08C07H17/065A61P39/06
CPCC07H1/00C07H1/08C07H17/065A61P39/06
Inventor 文连奎贺阳郑飞于寒松王治同吴曼毓王贲香卢思言刘贺李佳
Owner JILIN AGRICULTURAL UNIV
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