Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Capillary electrophoresis-enhanced light emitting diode-induced fluorescence detecting system and detecting method thereof

A technology of light-emitting diodes and capillary electrophoresis, which is applied in the directions of fluorescence/phosphorescence, measuring devices, material excitation analysis, etc., can solve the problems of low detection sensitivity and excitation intensity to be improved, and achieve the effect of improving detection sensitivity

Inactive Publication Date: 2019-08-20
四川中科微纳科技有限公司
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The above documents 1 to 4 all belong to the CE-LEDIF detection system in essence, but there are problems that the excitation intensity needs to be improved and the detection sensitivity is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Capillary electrophoresis-enhanced light emitting diode-induced fluorescence detecting system and detecting method thereof
  • Capillary electrophoresis-enhanced light emitting diode-induced fluorescence detecting system and detecting method thereof
  • Capillary electrophoresis-enhanced light emitting diode-induced fluorescence detecting system and detecting method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] see figure 1 , Figure 1a , a capillary electrophoresis-enhanced light-emitting diode-induced fluorescence detection system, mainly composed of a capillary column, an excitation light source, a convex lens, a conductive optical fiber, a silver-coated concave mirror, and a photomultiplier tube PMT;

[0039] Design concave silver mirror structure: through precise calculation of optical theory, set the best concave mirror curvature radius suitable for the detection wavelength range, design a concave mirror suitable for capillary column detection window with matching thickness; then select the concave mirror material and silver-plate it.

[0040] Concave silver mirror and capillary matching design, high-precision modular assembly: combined with the outer diameter and inner diameter of the capillary, and the focal point of the concave mirror (designed by precise calculation) is precisely coupled with the central axis of the capillary.

[0041] Silver-plated concave mirror: ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
thicknessaaaaaaaaaa
reflectanceaaaaaaaaaa
Login to View More

Abstract

The invention discloses a capillary electrophoresis-enhanced light emitting diode-induced fluorescence detecting system and a detecting method thereof. The system is mainly composed of a capillary column, an excitation light source, a convex lens, a conducting optical fiber, a silver-plated concave mirror, a photomultiplier tube PMT and other components, wherein the silver-plated concave mirror isarranged below a capillary detection window; the photomultiplier tube PMT is arranged above the capillary detection window and is located right above the silver-plated concave mirror for detecting sample emitting light converged and enhanced by the silver-plated concave mirror; and a detection recording system receives and processes the output signals of the photomultiplier tube PMT and records and displays the obtained detection result. Thus, through convergence of the silver-plated concave mirror, the sample emitting light-light propagating from different directions excited by the excitation light source are converged and reflected, the intensity and the brightness of the reflected light are further enhanced, and the detection sensitivity is finally greatly improved. Under equal other conditions, the sensitivity of the detection method is 30 times that of the conventional method.

Description

technical field [0001] The invention relates to a trace sample analysis and detection device, in particular to an enhanced light-emitting diode-induced fluorescence detection system and a related detection method. Background technique [0002] Capillary electrophoresis (CE) is an efficient separation and analysis technique. The separation of the analytes is driven by a high-voltage electric field with a difference in their charge-to-mass ratios. In recent years, capillary electrophoresis LED-induced fluorescence detection system has attracted more and more researchers because of its small size, low cost, high stability, and large wavelength range. Compared with conventional methods, it can be used to test a variety of samples such as (ribose nucleic acid) RNA. [0003] CE can match a variety of detectors, and the Chinese patent documents are disclosed as follows: [0004] Document 1 "A High Brightness Light-Emitting Diode Induced Fluorescence Detector" (CN 200410071057.7)...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 霍峰刘宇航蒋志李文琼胡猛
Owner 四川中科微纳科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com