Application of endothelial progenitor cells in transplantation treatment of coronary microembolization of mouse

A technology of endothelial progenitor cells and coronary arteries, applied in the application field of endothelial progenitor cells in the transplantation treatment of coronary artery microembolism, can solve the problems of lack of effective treatment methods for coronary artery microembolism, and achieve significant curative effect

Active Publication Date: 2019-08-23
BEIJING TSINGHUA CHANGGUNG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently there is no effective treatment for coronary microembolism

Method used

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  • Application of endothelial progenitor cells in transplantation treatment of coronary microembolization of mouse
  • Application of endothelial progenitor cells in transplantation treatment of coronary microembolization of mouse
  • Application of endothelial progenitor cells in transplantation treatment of coronary microembolization of mouse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation and culture identification of EPC

[0031] 1. Extraction of EPCs

[0032] 1) Take SD rats with a mass of 200-250 g and an age of 8-9 weeks, weigh them, and anesthetize them with 5% chloral hydrate (0.6 mL / 100 g) by intraperitoneal anesthesia. remove. The rats were fixed in prone position, depilated, sterilized with iodophor alcohol, and covered with towels. Remove both legs of the SD rat with scissors. All surgical operations are performed by the same person, in order to reduce unnecessary errors that may be brought about during the operation.

[0033] 2) Roughly separate the flesh and bones of the removed two legs into a 50 mL centrifuge tube filled with 75% absolute ethanol, and then place the centrifuge tube in a transfer chamber for UV disinfection for 10 minutes.

[0034] 3) Take the sterilized centrifuge tube to the ultra-clean bench, take out the two legs, separate the femur and tibia with sterilized forceps and small scissors, and cut bo...

Embodiment 2

[0049] Example 2: Establishment of Rat Coronary Artery Microembolism Model

[0050] In this example, a rat coronary microembolization model was established:

[0051] 1. Experimental grouping

[0052] 1) Sham operation group (Sham)

[0053] 2) Coronary microembolization group (CME)

[0054] 3) Coronary microembolization (CME) & EPC transplantation group (low dose group)

[0055] 4) Coronary microembolization (CME) & EPC transplantation group (high dose group)

[0056] 2. Animal modeling

[0057] Half an hour before the operation, 10% trichloroacetaldehyde was used to anesthetize the rats at a dose of 3 μL / g. After the rats were anesthetized, 0.5 mL of blood was collected from the tail vein, and blood clots were formed in a 37°C incubator. The rat tail was electrocoagulated to stop bleeding, the chest was shaved, the trachea was intubated, and the ventilator was connected. The breathing ratio was 1:2, and the tidal volume was 18. The breathing rhythm of the rat was consiste...

Embodiment 3

[0082] Example 3: Detection of the effect of EPC transplantation in the treatment of coronary micro-embolism in rats

[0083] 1. Experimental grouping: the same as in Example 2.

[0084] 2. Animal modeling: the same as in Example 2.

[0085] 3 Immunohistochemical detection

[0086] 3.1 Preprocessing

[0087] Paraffin sections: dewaxed and hydrated according to conventional methods, soaked in xylene for 5 min, replaced xylene and then soaked for 5 min; soaked in absolute ethanol for 5 min; soaked in 95% ethanol for 5 min; soaked in 85% ethanol for 5 min ; Soak in 70% ethanol for 5 min, and soak in distilled water for 3 min × 3 times.

[0088] 3.2 Experimental steps

[0089] 1) Bake the slices at 37℃ overnight;

[0090] 2) Xylene 1, 2, 3 for 10min each, ethanol 100%, 95%, 90%, 80%, 70%, 50% for 2min each, distilled water for 1min;

[0091] 3) TBS wash 3 times, 5min each time;

[0092] 4) Antigen retrieval (high fire to boiling, turn to low fire for 20min), natural cooling...

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Abstract

The invention relates to treatment for coronary microembolization. The coronary microembolization (CME) is an extremely-common and important clinical event in acute coronary syndrome and percutaneouscoronary intervention therapy. According to the treatment, it is discovered that through low-dose EPC transplantation, generation of endothelial cell growth factors VEGF and blood capillaries can be increased, fibrocyte growth factors bFGF are inhibited, cardiac muscular tissue lesion and cardiac muscular tissue collagen fiber proliferation caused by CME are relieved, and a new strategy is provided for CME treatment.

Description

technical field [0001] The present invention relates to the treatment of coronary micro-embolism, in particular to the application of endothelial progenitor cells in the treatment of coronary micro-embolism by transplantation. Background technique [0002] Coronary atherosclerotic plaque rupture leads to platelet activation and aggregation to form thrombus, and thrombus completely occludes the larger coronary blood vessels in the epicardium, which is an important pathogenesis of acute myocardial infarction (ami). Plaque fragments falling into distal small vessels can directly or secondary to cause distal vascular occlusion, namely coronary microembolism (CME). [0003] Microthrombi can often be found in the coronary microcirculation (mostly with an inner diameter of 10-100 μm) in patients who died of ischemic heart disease. This coronary microthrombosis is characterized by platelet aggregation, fibrinogen, and the formation of atherosclerotic substances (including cholester...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/44A61P7/02A61P9/10
CPCA61K35/44A61P7/02A61P9/10
Inventor 薛亚军张萍吴建缪国斌李锟周杰吕婷婷耿雨张鸥
Owner BEIJING TSINGHUA CHANGGUNG HOSPITAL
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