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Detection method of indazole derivatives

A detection method and derivative technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve problems such as the lack of new psychoactive substances, and achieve the effects of easy popularization and application, fast method, and easy operation.

Inactive Publication Date: 2019-08-23
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, research on new psychoactive substances in our country is still very scarce, and the standard detection method for the qualitative and quantitative detection of controlled indazole amides synthetic cannabinoids is still blank. sue for technical support

Method used

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  • Detection method of indazole derivatives
  • Detection method of indazole derivatives
  • Detection method of indazole derivatives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Embodiment 1. Gas chromatography-mass spectrometry detection (qualitative analysis) of indazole derivatives in the sample

[0097] 1. Qualitative analysis of 5F-AMB in samples

[0098] 1) Sample preparation

[0099] After the sample was homogenized, weigh 5 mg and put it in a centrifuge tube, add 10 mL of methanol, dissolve it by ultrasonic, vortex mix, filter with a 0.45 μm filter membrane, and use it for analysis. According to the concentration of the target substance in the sample solution, the weighing amount of the sample can be adjusted appropriately.

[0100] 2) Gas chromatography-mass spectrometry (GC-MS) reference conditions

[0101] a) Instrument: GC-MS.

[0102] b) Ion source: electron bombardment source (EI).

[0103] c) Mass range: 35amu~500amu.

[0104] d) Acquisition method: full scan (Scan).

[0105] e) Column type: DB-5MS quartz glass capillary column (5% phenyl + 95% polydimethylsiloxane) or other equivalent columns.

[0106] f) Column parameter...

Embodiment 2

[0128] Embodiment 2, to the gas chromatographic detection (external standard single-point method) of indazole derivatives in the sample

[0129] 1. Quantitative analysis of 5F-AMB in samples

[0130] 1) Preparation of standard solution

[0131] 1.1) Preparation of 1.0mg / mL 5F-AMB standard stock solution

[0132] Accurately weigh a certain amount of 5F-AMB standard substance, dissolve it in methanol, prepare a 1.0 mg / mL 5F-AMB standard stock solution, and store it in a refrigerator for 6 months.

[0133] 1.2) Preparation of 0.1mg / mL 5F-AMB standard working solution

[0134] Accurately pipette an appropriate amount of 1.0mg / mL 5F-AMB standard stock solution, dilute to 0.1mg / mL with methanol, shake and mix well, and store in the refrigerator for 3 months.

[0135] 2) Reference conditions for gas chromatography (GC-FID)

[0136] a) Instrument: GC.

[0137] b) Detector: FID.

[0138] c) Chromatographic column model: DB-5 quartz glass capillary column (5% phenyl + 95% polydime...

Embodiment 3

[0188] Embodiment 3, to the gas chromatography detection (standard working curve method) of indazole derivative in the sample

[0189] 1. Quantitative analysis of 5F-AMB in samples

[0190] 1) Standard working curve drawing

[0191] Accurately weigh a certain amount of 5F-AMB standard substance, dissolve it in methanol, make a standard stock solution with a concentration of 3mg / mL, store it in a sealed refrigerator at 0-5°C, and have a validity period of 6 months. Accurately pipette appropriate amount of 5F-AMB standard stock solution, dilute with methanol to 1.5, 0.9, 0.3, 0.09, 0.03mg / mL concentration, and mix thoroughly. 6 concentrations of 3.0, 1.5, 0.9, 0.3, 0.09, and 0.03 mg / mL were used to draw a 5F-AMB standard working curve with a linear range of 0.03-3.0 mg / mL.

[0192] 2) quantitative

[0193] 2.1) Extraction operation

[0194] Weigh 2 samples in parallel, about 30 mg each, add 10 mL of methanol, ultrasonically dissolve, analyze with GC-FID after centrifugation,...

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Abstract

The invention discloses a detection method of indazole derivatives. The detection method comprises a qualitative detection method of gas chromatography-mass spectrometry, a qualitative detection method of gas chromatography and a quantitative detection method of gas chromatography, and is suitable for qualitative and quantitative detection and identification of indazole derivatives in solid test materials for drug cases. According to the invention, a qualitative and quantitative analysis method for the indazolamide synthetic cannabinoid is established by utilizing the commonly used gas chromatography and gas chromatography-mass spectrometry technology, and the method is quick, simple, convenient and easy to operate, is convenient to popularize and apply, can meet the requirements of judicial identification departments on the handling of such cases, and provides a basis for the smooth litigation of such cases.

Description

technical field [0001] The invention relates to a detection method of indazole derivatives. Background technique [0002] New psychoactive substances, English name New psychoactive substances, referred to as NPS, early called Legal Highs or Designer Drug, Chinese called "legal stimulants" or "planning drugs", is the research and production of some controlled drugs in order to avoid crackdowns. The drug analogs obtained by structural modification have similar effects to controlled drugs, and some have stronger excitatory, hallucinogenic, and anesthetic effects. The United Nations Office on Drugs and Crime (UNODC) divides new psychoactive substances into 9 categories, namely: synthetic cannabinoids, cathinones, phenethylamines, tryptamines, piperazines, ketamines and benzene rings. Ridines, aminoindenes, plants and others. Among them, synthetic cannabinoids and cathinones are the most abused NPS. [0003] Synthetic cannabinoids are the most abused new psychoactive substance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/04G01N30/06G01N30/68G01N30/86
CPCG01N30/02G01N30/04G01N30/06G01N30/68G01N30/72G01N30/8634G01N2030/047
Inventor 钱振华郑晓雨赵彦彪
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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