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Chemoenzymatic synthesis method of monosialotetrahexosylganglioside with different lipid chain lengths

A ganglioside, monosialic acid technology, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of high cost and long synthesis cycle, and achieve high success rate, short synthesis cycle, and economical The effect of synthesis cost

Inactive Publication Date: 2019-08-27
武汉糖智药业有限公司
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  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the shortcomings of long synthesis period and high cost in the prior art, and propose a chemical and enzymatic synthesis method of monosialotetrahexosyl gangliosides with different lipid chain lengths

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  • Chemoenzymatic synthesis method of monosialotetrahexosylganglioside with different lipid chain lengths

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Embodiment Construction

[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0029] refer to figure 1 , a method for synthesizing monosialotetrahexosyl gangliosides with different lipid chain lengths by chemical enzymatic method, comprising the following steps;

[0030] Step 1: Pig brain degreasing; first, prepare fresh goods or frozen pig brain at minus 25 degrees, tear off the meninges and surface blood vessels, weigh them and cut them into small pieces, then add acetone at 4L / kg to prepare a homogenate, stir for 30 Carry out suction filtration after 10 minutes, wherein, filtrate stays to reclaim acetone, collects insoluble matter for subsequent use;

[0031] Step 2: Extract the insoluble matter; first, add 5 times the volume of chloroform...

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Abstract

The invention relates to the technical field of drug synthesis, in particular to a chemoenzymatic synthesis method of monosialotetrahexosylganglioside with different lipid chain lengths. The synthesisof the monosialotetrahexosylganglioside is realized through several steps of pig brain degreasing, insoluble matter extraction, concentration treatment, packing treatment, sample loading and elution,and sample collection and treatment. According to the method involved in the invention, the monosialotetrahexosylganglioside is separated and purified through existing materials of pig brains, the synthesis cycle of a whole process is relatively short, the operation requirement is relatively low, and the success rate is high; meanwhile, old deae-sepharose after use is subjected to secondary treatment, so that the cost of chemical reagents can be reduced to a large extent.

Description

technical field [0001] The invention relates to the technical field of drug synthesis, in particular to a method for synthesizing monosialotetrahexosyl gangliosides with different lipid chain lengths by chemical enzymatic method. Background technique [0002] Paralysis, dementia, and loss of living ability caused by various reasons of brain and nerve damage have brought pain and burden to tens of millions of patients. Therefore, research on reducing the disability rate and promoting neurological function recovery is of great significance; One of the encouraging research results is the clinical application of gangliosides, a kind of glycosphingolipids, derivatives of a class of hydrophilic fatty acids, which are the constituents of most mammalian cell membranes, It is particularly abundant in the system, and studies have shown that it plays an important role in reducing experimental cerebral edema, increasing dendrite generation and survival rate of nerve cells after trauma, ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/10C07H1/08
CPCC07H1/08C07H15/10
Inventor 王鹏杨广宇
Owner 武汉糖智药业有限公司