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An ultra-microinjection detection and control device and method based on lensless imaging technology

A control device and lens-less technology, which is applied in the direction of using micro-injection method, measuring device, enzymology/microbiology device, etc., can solve the problem of lack of accurate detection of capillary glass needles, etc., and achieve simple structure, low cost, and technical maturity high effect

Active Publication Date: 2020-03-27
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the existing problems of lacking a device capable of accurately detecting the change of the liquid level in the capillary glass needle and precisely controlling the volume of the injected liquid

Method used

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  • An ultra-microinjection detection and control device and method based on lensless imaging technology
  • An ultra-microinjection detection and control device and method based on lensless imaging technology
  • An ultra-microinjection detection and control device and method based on lensless imaging technology

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Embodiment 1

[0046] In the present invention, the miniature line array image sensing chip can be the model S10226 linear CMOS image sensor produced by Hamamatsu as the detection chip, the number of sensing bits is 12 bits, the photosensitive area specification is a single pixel 7×125 μm, and the line array has a total of 1024 pixels , the total length of the photosensitive area is 8mm, and the overall dimensions are 9.1mm×2.4mm×1.6mm. The surface of the sensor is covered with a light-reducing film that reduces light by 90%, and the external light source is composed of LED light-emitting elements and light-guiding fibers, and the light intensity is about 150lux. The glass injection needle can be drawn from a capillary glass tube with an outer diameter of 1 mm and an inner diameter of 0.5 mm. The pneumatic microsyringe pump can be used with the FemtoJet 4i upgrade microsyringe pump produced by Eppendorf.

[0047] In this embodiment, it is assumed that the liquid surface light can saturate t...

specific Embodiment approach 2

[0053] Specific Embodiment 2: This embodiment is a further description of the ultra-microinjection detection and control device based on lensless imaging technology described in Specific Embodiment 1. In this embodiment, the parallel light source 4 includes LED light-emitting elements, guide The optical fiber and the light-homogeneous film, the LED light-emitting element, the light-guiding fiber and the light-homogeneous film are laminated and arranged sequentially from the outside to the inside.

specific Embodiment approach 3

[0054] Embodiment 3: This embodiment is a further description of the ultra-microinjection detection and control device based on lensless imaging technology described in Embodiment 1. In this embodiment, the control unit includes a drive circuit 3 and a flexible circuit board 2, the driving circuit 3 is arranged on the needle holder e, the driving circuit 3 is connected to the micro linear array image sensing chip 5 through the flexible circuit board 2, and the flexible circuit board 2 is used to collect the measurement information of the micro linear array image sensing chip 5 According to the information, the injection pressure of the injection pump is corrected, and the driving circuit 3 is used to drive the injection pump to work according to the injection pressure sent by the flexible circuit board 2 .

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Abstract

An ultra-microinjection detection and control device based on lensless imaging technology and a method thereof, relating to the field of lensless imaging technology and ultra-microinjection control technology of a microinjection system. The invention aims to solve the existing problems of lacking a device capable of accurately detecting the position change of the liquid level in the capillary glass needle and precisely controlling the volume of the injected liquid. The lensless optical liquid level sensor is used to measure the liquid level change in the injection needle; the micro injection control unit is used to track the liquid level change and correct the injection pressure of the syringe pump; the transmitted light generated by the parallel light source penetrates the transparent glass injection needle tube , the intensity of the parallel light source is weakened to within the photosensitive range of the micro linear array image sensor chip through the light reducing film, and finally injected into the micro linear array image sensor chip, and the liquid level in the injection needle is measured by the micro linear array image sensor chip Variety. It is used to measure the liquid level change in the glass needle, and correct the pressure of the syringe pump to stabilize the injection liquid in the syringe.

Description

technical field [0001] The present invention relates to a lens-free imaging technology and an ultra-micro injection control technology used in a microinjection system in the field of life sciences, and specifically relates to a method of imaging a liquid surface in a capillary glass injection needle by using a linear array image sensor without a lens. A new method of measuring its position change to perform closed-loop control on the micro-injection pump is mainly used in the real-time detection of the liquid level in the needle tube during the micro-injection of tiny organisms such as embryos, cells, and zebrafish larvae. Feedback is thus generated to achieve precise control of the injection volume. Background technique [0002] With the advancement of life science technology, genetic, drug and developmental studies based on embryos, cells and tiny organisms such as zebrafish larvae, nematodes and fruit flies have become a commonly used research method. Since these experim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36C12M1/34C12Q3/00C12N15/89G01F23/292
CPCC12M41/44C12M41/48C12Q3/00C12N15/89G01F23/292C12M33/04C12M41/36A61M5/3286A61M2205/3348A61M2205/3334A61M5/141A61M2005/1585A61M2005/1588A61M5/16886G01F23/804A61M5/16831
Inventor 佟明斯于兴虎高会军张林琦
Owner HARBIN INST OF TECH
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