Application of clam active polypeptide extracted by ultrasonic waves
An active polypeptide and ultrasonic technology, which is applied in the direction of food ultrasonic treatment, application, and the function of food ingredients, etc., can solve the problems of environmental pollution, difficult processing, waste of resources, etc., achieve strong umami taste, increase utilization value, and improve amino acids. Kind of effect
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Embodiment 1
[0015] The use of the clam active polypeptide extracted by ultrasonic waves, the application of the clam polypeptide in the preparation of seasoning sauce for enhancing the umami taste of food, and the application of the clam polypeptide in the preparation of mushroom sauce for enhancing the umami taste of food.
[0016] Preparation of clam active polypeptide: Take clams, wash and remove shells, break the tissue, add deionized water, deionized water contains 0.06wt% p-methoxyethylphenol, solid-liquid ratio 1:9, at -20 ℃ and 35 ℃, repeated freezing and thawing 4 times, 2 hours each time, ultrasonic treatment, ultrasonic power 350w, time 4min, adjust pH 7.6, at a temperature of 46 ℃, add trypsin, solid-liquid ratio 1:3 (V / W), Hydrolyze for 3 hours, raise the temperature to 58 ℃, adjust the pH to 6.0, the substrate ratio is 1:2.8 (V / W), add papain, papain contains 0.01wt% D-alloisoleucine and L-alloisoleucine A mixture of acids, the mass ratio of D-alloisoleucine and L-alloisoleu...
Embodiment 2
[0018] Use of the clam active polypeptide extracted by ultrasonic waves. The clam polypeptide is used in the preparation of seasoning sauce for enhancing the umami taste of food. The clam polypeptide is preferably used in the preparation of chili sauce for enhancing the umami taste of food.
[0019] The preferred preparation of clam active polypeptides: take clams, wash and remove their shells, break the tissue, add deionized water, which contains 0.06wt% p-methoxyethylphenol, and the solid-to-liquid ratio is 1:10. 35°C, repeated freezing and thawing 5 times, each time 3h, ultrasonic treatment, ultrasonic power 400w, time 5min, adjust pH 7.8, at 45°C, add trypsin, solid-liquid ratio 1:4 (V / W) , hydrolyzed for 3 hours, heated to 60 ℃, adjusted to pH 5.8, substrate ratio 1:2.6 (V / W), added papain, papain contained 0.02wt% D-alloisoleucine and L-alloisoleucine A mixture of amino acids, the mass ratio of D-alloisoleucine and L-alloisoleucine is 65:0.06, hydrolyzed for 3 hours, the...
Embodiment 3
[0022] The use of the clam active polypeptide extracted by ultrasonic waves, the application of the clam polypeptide in the preparation of seasoning sauce for enhancing the umami taste of food, and the application of the clam polypeptide in the preparation of chili sauce for enhancing the umami taste of food.
[0023] Preparation of clam active polypeptide: take clams, wash and remove shells, break the tissue, add deionized water, deionized water contains 0.05wt% p-methoxyethylphenol, solid-liquid ratio 1:9, at -20 ℃ and 35 ℃, repeated freezing and thawing 4 times, 2 hours each time, ultrasonic treatment, ultrasonic power 500w, time 4min, adjust pH 7.8, at a temperature of 45 ℃, add trypsin, solid-liquid ratio 1:3 (V / W), Hydrolyze for 5 hours, heat up to 58°C, adjust pH to 5.8, substrate ratio 1:2.7 (V / W), add papain, papain contains 0.02wt% D-alloisoleucine and L-alloisoleucine A mixture of acids, the mass ratio of D-alloisoleucine and L-alloisoleucine is 64:0.07, hydrolyzed ...
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