Iodine-125 particle and natural killer cell combined product capable of treating liver cancer and application method of Iodine-125 particle and natural killer cell combined product
A combination product, a technology for treating liver cancer, which can be used in drug combinations, preparations for in vivo testing, and pharmaceutical formulations, and can solve problems such as liver cancer recurrence
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] Example 1: KIR Mismatch Donor Selection
[0030] The invention adopts a human HANK cell in vitro preparation kit to induce KIR-mismatched HANK cell expansion and activation. For allogeneic HANK cell immunotherapy, an allogeneic donor (preferably a relative of the patient) is selected based on the mismatch between the patient's peripheral blood inhibitory KIR and the donor's HLA class I molecular genotype. Using TIANamp Blood DNA Kit DNA Kit (Tianjin Xiupeng Biotechnology Co., Ltd., Tianjin, China) and KIR / HLA-Cw Genotyping Low Resolution Kit (PCR-SSP; SuperBio.Developing Co., Tianjin, China) Test donors and patients. According to previously published criteria, a KIR / HLA-Cw mismatch was defined as the absence of one or more alleles of HLA as an inhibitory KIR ligand.
Embodiment 2
[0031] Embodiment 2: HANK cell preparation
[0032] HANK cells were prepared under GMP standard conditions (Good Manufacturing Practice) with clinical grade reagents, Human NK Cell In Vitro Culture Synergy Kit (Hanke Bioengineering Co., Ltd., Shenzhen, China) was used to stimulate blood mononuclear cells (PBMC) NK cell expansion and activation. First, the collected blood (50-80 mL) was transferred to a 50 mL conical tube and centrifuged at 600×g for 15 minutes. Collect the plasma in the supernatant into a 50 mL conical tube. Place the conical tube in a 56°C water bath, take it out after 30 minutes and cool it down to a tube temperature of 37°C; centrifuge at 400×g for 10 minutes, then transfer the supernatant to a new 50mL conical tube and store it at 4°C for subsequent use. use in one step. Plasma stored at 4°C for more than 12 hours should be centrifuged again and only the supernatant should be used. (2) Add the same volume of normal saline to the blood cells at the bott...
Embodiment 3
[0037] Example 3: Patient Treatment Protocol
[0038] A 48-year-old male patient with advanced HCC had recurrence of liver cancer after liver transplantation.
[0039]Iodine 125 seed implantation was performed in June 2016. According to the results of computed tomography (CT), the number of iodine-125 particles required was determined according to the size of the tumor, and strands were prepared for transplantation. After local anesthesia and electrocardiographic monitoring of the patient in the supine position, 10 particles, each with an activity of 0.7mCi and a half-life of 60.1 days, were implanted into the edge of the tumor at an interval of 0.5cm.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More