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Bacillus velezensis for preventing and treating clubroot disease and application of Bacillus velezensis

A technology for bacillus and clubroot, which is applied in the field of agricultural microorganisms and can solve problems such as poor stability, low control effect of clubroot, and easy inactivation.

Inactive Publication Date: 2019-08-30
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a new strain for the prevention and treatment of the defects in the prior art, especially for the defects of the existing Bacillus Velez that are not effective in preventing clubroot, are easily inactivated, and have poor stability. Bacillus Veles

Method used

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  • Bacillus velezensis for preventing and treating clubroot disease and application of Bacillus velezensis
  • Bacillus velezensis for preventing and treating clubroot disease and application of Bacillus velezensis
  • Bacillus velezensis for preventing and treating clubroot disease and application of Bacillus velezensis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Isolation identification and fermentation culture of embodiment 1 bacterial strain

[0012] 1. Strain screening

[0013] The microorganism of the present invention is obtained by separating from healthy rapeseed root soil in a field with severe clubroot disease in the Hubei Provincial Key Laboratory of Crop Disease Detection and Safety Control, Huazhong Agricultural University, Wuhan City, Hubei Province. Take 200 μL soil dilution (10 -3 、10 -4 、10 -5 and 10 -6 ) was added to the Martin medium plate, and the growth of the colony was observed every day. According to the characteristics of colony color, shape, wet and dry, transparency and edge, a representative single colony was selected for purification and culture, and the obtained pure culture strain was used on the slant of PDA medium Store the tubes at 4°C. Since Plasmodium is an obligate parasite and cannot be cultured artificially, fungi similar to the cell wall components of Plasmodium are used as indicator ...

Embodiment 2

[0022] Example 2: Inhibitory effect of Bacillus Velez F85 on vigor and germination of Plasmodium dormant spores

[0023] 1. Inhibitory effect of Bacillus Velez F85 on the activity of dormant spores of Plasmodium

[0024] Dilute the resting spore suspension of Plasmodium to 10 9 Individuals / mL, take an equal volume of dormant spore suspension and Bacillus velesi F85 sterile fermentation broth in a sterile centrifuge tube, and mix evenly; replace Bacillus velesii F85 aseptic fermentation solution was used as a blank control, and fluazinam diluted 1000 times by chemical agents was set as a positive control. Three repetitions were set for each treatment, and all treatments were left standing at room temperature for 2 days. After 2 days, the above-mentioned treatments were uniformly mixed with an equal volume of pH 7.4 phosphate buffer, and 1 / 2 of the total volume of 2% Evans blue solution was added, and after mixing, it was placed at room temperature for 30 minutes. Take a smal...

Embodiment 3

[0033] Example 3: Inhibitory effect of Bacillus velei F85 on root hairs of rapeseed root infection by Plasmodium

[0034] Fill the 50mL beaker with sterilized coarse sand, and wrap it with black paper to form a dark environment to facilitate the germination and infection of dormant spores. The rapeseed seedlings that had germinated for 4 days were moved into beakers (5 plants per beaker). After slowing down the seedlings for 3 days, inoculate 10% Bacillus Velez F85 aseptic fermentation filtrate and Plasmodium dormant spore suspension (final concentration is 10 7 individuals / mL), and inoculated with the same concentration of dormant spore suspension as the control. The plant nutrient solution (pH=6.3) was watered timely, and all treatments were cultured in a plant culture box at 24°C, with a photoperiod of 16 / 8h and a relative humidity of 90%. From the 1st to 15th day after inoculation, samples were taken every day, and the roots were washed and fixed in acetic acid: ethanol ...

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Abstract

The invention discloses a Bacillus velezensis F85 for preventing and treating clubroot disease. The Bacillus velezensis F85 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2019105. The strain has inhibitory effect on both the vitality and germination of resting spores of plasmodiophoromycetes, and can significantly inhibit the infection of plasmodiophoromycetes to rape root hairs. Pot experiments show that root irrigation treatment with the Bacillus velezensis F85 fermentation broth can effectively prevent and treat the clubroot disease of rapes, the comprehensive preventing effect reaches 83.4%, the treatment is at a higher level at present, and the strain also has the advantages of stable effect, wide environmental adaptability and etc.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a strain of biocontrol Bacillus velezensis and its application in the prevention and treatment of rapeseed clubroot. Background technique [0002] Cruciferous clubroot is a worldwide soil-borne disease caused by Plasmodiophora brassica infection. Clubroot bacteria can survive in the soil for more than 20 years, and can infect more than 100 species of cruciferous plants. Once clubroot occurs, the pathogenic bacteria will continue to accumulate, and the source of primary infection will continue to increase. Swelling disease has become one of the important diseases of cruciferous plants, which seriously restricts the sustainable development of oilseed and vegetable industry and the increase of farmers' income. After the root of rapeseed is infected, the root cells proliferate abnormally, forming tumor-like protrusions of different sizes. In the later...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 郑露朱曼丽何有为黄俊斌李仪任体容
Owner HUAZHONG AGRI UNIV
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