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Bacillus velezensis and application thereof to preventing and treating tomato phytophthora root rot

A technology of bacillus and tomato, which is applied in the field of bacillus velei and its application in the prevention and treatment of tomato Phytophthora root rot, which can solve the problems of environmental pollution, poor degradation, and difficulty in breeding for disease resistance

Active Publication Date: 2019-08-30
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the control methods of Phytophthora root rot of tomato. The selection of disease-resistant varieties is an economical and effective control measure, but the work of disease-resistant breeding is relatively difficult, and traditional agricultural measures have limitations. Although it is simple, fast and effective, its degradability is poor, and it is easy to cause environmental pollution, and the long-term single use of similar chemical agents such as metalaxyl has made the problem of drug resistance increasingly prominent. Therefore, a green control measure is urgently needed in production to ensure Sustainable Development of Vegetable Industry

Method used

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  • Bacillus velezensis and application thereof to preventing and treating tomato phytophthora root rot
  • Bacillus velezensis and application thereof to preventing and treating tomato phytophthora root rot
  • Bacillus velezensis and application thereof to preventing and treating tomato phytophthora root rot

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Experimental program
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Effect test

preparation example Construction

[0060] The preparation of the chemicals involved in the following examples is all commonly used medicines in this area, such as:

[0061] SolutionⅠ: 10mmol -1 phosphoric acid.

[0062] Solution II: 1mL 0.5mol·L -1 FeCl 3 Dissolve in 50mL 35% HClO 4 .

Embodiment 1

[0063] Embodiment 1, the isolation, purification and identification of Bacillus velezensis ZF50

[0064] 1. Isolation of Bacillus

[0065] Weigh 10g soil sample from tomato soil collected from Shouguang, Shandong Province for many years, put it into a triangular flask filled with 100mL sterile water, place it on a shaker at 27°C and 200r / min for 30min, and make it fully mixed. Dilute 1mL of the bacteria-soil suspension to 10 min at 80°C in a water bath -1 , 10 -2 , 10 -3 , 10 -4 100 μL of each gradient suspension was dropped onto the center of the LB plate, spread evenly with a spreader to make the plate fully absorbed, repeat each gradient 3 times, and place the plate upside down in a 30°C constant temperature incubator for 2 days. Bacterial colonies with different shapes were picked and purified by streaking on the plate, and then numbered and stored. A total of 98 Bacillus strains were isolated.

[0066] 2. Screening of Bacillus

[0067] Use a sterilized pipette tip t...

Embodiment 2

[0090] Embodiment 2, Bacillus velezensis (Bacillus velezensis) ZF50 are to the mensuration of six kinds of pathogenic fungus antibacterial effects

[0091] 24 hours after the pathogenic fungi were inoculated on the PDA plate, 5 μL of Bacillus velezensis ZF50 bacterial suspension (1.0 × 10 8 cfu mL -1 ), do 3 repetitions, set up a blank control only inoculated with liquid LB medium, and culture at 27°C for 5 days. When the blank control is about to cover the entire petri dish, measure the control growth (colony diameter) and treatment growth (growth diameter after inoculation of bacteria) of the target bacteria, and use the bacteriostatic rate to represent.

[0092] Inhibition rate (%)=(control colony diameter-treatment colony diameter) / control colony diameter×100%

[0093] The results show( Figure 4 , table 3), Bacillus velezensis (Bacillus velezensis) ZF50 all has good bacteriostatic effect to 6 kinds of testing pathogenic bacteria, wherein the bacteriostatic effect is th...

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Abstract

The invention discloses bacillus velezensis and an application thereof to preventing and treating tomato phytophthora root rot. The invention provides bacillus velezensis (bacillus velezensis) ZF50, and the preservation number of the bacillus velezensis in the China General Microbiological Culture Collection Center is CMCC No. 16775. The invention provides an efficient biocontrol strain for preventing and treating the tomato phytophthora root rot, and bacillus velezensis has important production significance for a green prevention and control technique of tomato diseases and promotion of sustainable development of the tomato industry.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a strain of bacillus velei and its application in preventing and treating Phytophthora root rot of tomato. Background technique [0002] Tomato Phytophthora root rot is a devastating fungal soil-borne disease caused by Phytophthora capsici Leonian. Only 7-15 days, the incidence rate is 50%, or even out of production. It is one of the important diseases of tomato production in protected areas and open fields in many countries, which seriously restricts the development of tomato industry. At present, there are few reports on the control methods of Phytophthora root rot of tomato. The selection of disease-resistant varieties is an economical and effective control measure, but the work of disease-resistant breeding is relatively difficult, and traditional agricultural measures have limitations. Although it is simple, fast and effective, its degradability...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 李宝聚谢学文孙雪莹李磊石延霞柴阿丽
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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