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Retrieval Method of Microcystin mc-lr Concentration in Water Body

A microcystin, MC-LR technology, applied in material excitation analysis, fluorescence/phosphorescence, instruments, etc., can solve the problem that the inversion model cannot be used universally, and achieve the effect of rapid detection, good accuracy and stability

Active Publication Date: 2022-02-11
YANGZHOU UNIV
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Problems solved by technology

Therefore, the inversion model cannot be generalized

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  • Retrieval Method of Microcystin mc-lr Concentration in Water Body
  • Retrieval Method of Microcystin mc-lr Concentration in Water Body
  • Retrieval Method of Microcystin mc-lr Concentration in Water Body

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Embodiment Construction

[0032] The present invention is further clarified in conjunction with specific embodiments.

[0033] Detection method of microcystin Mc-LR in the water body, including:

[0034] (1) Sampling: Copper green microcyst algae breaking period, in Wuxi Taihu Monitoring point with sampler 20 ~ 30 cm underwater 20 ~ 30 cm, the amount of sampling is 31. 9 samples were randomly extracted as the sample to be inverse, and other samples were used to build inverse equations.

[0035] (2) Refer to the national standard GB / T20466-2006 method, the Agilent 1200 Liquid Chromatograph is used to determine the content of the microcystin toxin MC-LR in the water sample sample, and the establishment of the inversion equation and the inversion detection;

[0036] (3) Extraction of copper-green microcystyllavine extracellular organics in water samples: divide the extracellular organism of copper green microcystis into DEOM (dissolved extracellular organism) and BEOM (binding extracellular organic matter) ...

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Abstract

The invention discloses an inversion method for the concentration of Microcystin MC‑LR in water bodies, comprising: taking the normalized characteristic fluorescence peak intensity of Microcystis aeruginosa extracellular organic matter in water samples as an independent variable, and normalizing Concentration of microcystin MC-LR was used as the dependent variable to construct a one-variable linear inversion equation, wherein the characteristic fluorescence peak intensity and concentration of microcystin MC-LR were normalized by the concentration of chlorophyll a in the water sample; For the detected water samples, measure the three-dimensional excitation emission matrix fluorescence spectrum of the extracellular organic matter of Microcystis aeruginosa in the water sample, obtain the characteristic fluorescence peak intensity of the extracellular organic matter of Microcystis aeruginosa, and then substitute it into the unary linear inversion equation to obtain the microcystis Concentration of phycotoxin MC‑LR. This method does not require sample pretreatment, and can realize rapid on-line monitoring of the concentration of microcystin MC‑LR. The characteristic fluorescence peak intensity of extracellular organic matter of Microcystis aeruginosa is used as a variable in the construction equation, which provides a new idea for the inversion method.

Description

Technical field [0001] The present invention relates to a hydrostatic environmental detection, and more particularly to the inversion method of microcystin Mc-LR concentration in a water body. Background technique [0002] A large amount of microcystin Mc-LR enters the water body and seriously threatened drinking water safety. Microcystin Mc-LR is mainly produced by copper green sac in the water body. At present, the conventional concentration detection method is divided into biological assay, physical chemical detection, biochemical detection method, molecular biological testing, and the test method, the water samples are cumbersome, time consumption, high cost, unable to achieve rapid online monitoring. The inversion method can utilize the inversion model of the inversion model and the concentration of microcylacetoxin MC-LR in the indirect reactive water. Many research scholars use a nutrient concentration (such as nitrogen, phosphorus, etc.), temperature, and dissolved oxygen...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 王志刚王赛赛张晴静孙宇
Owner YANGZHOU UNIV
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