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Molecular genetic marker related to linear movement of boar sperm and its application and acquisition method thereof

A molecular genetic marker, linear motion technology, applied in the field of molecular markers, can solve the problems of high computational intensity, unreasonable setting of significance threshold, overestimation of marker effect, etc., to improve the success rate and improve the ability of sperm linear motion. Effect

Active Publication Date: 2019-09-03
广西贵港秀博基因科技股份有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

Such methods often face problems such as high computational intensity, overestimation of marker effects, and unreasonable setting of significance thresholds.

Method used

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  • Molecular genetic marker related to linear movement of boar sperm and its application and acquisition method thereof
  • Molecular genetic marker related to linear movement of boar sperm and its application and acquisition method thereof
  • Molecular genetic marker related to linear movement of boar sperm and its application and acquisition method thereof

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Experimental program
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Effect test

Embodiment 1

[0056] 1. Phenotype-pedigree data collection

[0057] The basic research population of this application is Duroc boars, all of which come from the boar station of Guangxi Xiubo Co., Ltd. The complete pedigree contains 12 generations of 5,284 breeding pigs, of which 2,693 boars recorded the linear movement traits of sperm from 2015 to 2018 Phenotypic data; sperm linear motion was obtained by analyzing fresh semen with the UltiMateTM CASA (Hamilton Thorne Inc., Beverly, MA, USA) system. A total of 143,114 semen trait observations (average 53 data per boar) were obtained for phenotype-genotype association analysis.

[0058] 2. Genotyping and quality control

[0059] The ear tissue samples or blood samples of 1733 boars were collected, the total DNA was extracted, and genotyping was performed using the GGP 50k SNP (GeneSeek, US) chip, and 50705 SNP markers covering the whole genome were obtained. According to the latest version of the pig reference genome (Sscrofa11.1), the phys...

Embodiment 2

[0102] According to the results of the genes screened in Example 1, it is shown that the molecular genetic markers related to the linear movement of boar sperm in the present application are located at the 136112947bp position of pig chromosome 15, which is a T>C mutation (Sscrofa10 .2), corresponding to the 101st nucleic acid site in the nucleic acid sequence table SEQ ID NO.1.

Embodiment 3

[0104] According to the results of the genes screened in Example 1, it is shown that the molecular genetic marker related to the linear movement of boar sperm in this application is located at the 18505448bp position of pig chromosome 3, which is a G>A mutation (Sscrofa10.2), corresponding to the 101st nucleic acid site located in the nucleic acid sequence table SEQ ID NO.2.

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Abstract

The invention relates to the technical field of molecular markers, in particular to a molecular genetic marker related to linear motion of boar sperm, and an application and a method thereof. The method analyzes the linear motion traits of boar sperm by genome-wide association study (wssGWAS) analysis, and obtains three molecular genetic markers related to linear motion of boar sperm, which are located at the 136112947 bp position of chromosome 15 of pig, and is a mutation that T is greater than C; at the 18505448 bp position of chromosome 3 of pig, and is a mutation that A is greater than G;and at the 63272581 bp position of chromosome 11 of pig, and is a mutation that C is greater than T. The method used to analyze the linear motion of boar sperm is simple, efficient and fast.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of molecular markers, in particular to molecular genetic markers related to the linear movement of boar sperm and its application and acquisition method. [0003] 【Background technique】 [0004] In recent years, with the large-scale and intensive development of pig farming, genetic improvement has a greater impact on production efficiency. Over the past few decades, more and more farms have used fresh semen for artificial insemination. Artificial insemination has thus become a powerful tool in countries where pig production is highly intensive. The fertilization ability of each dose of semen is closely related to its sperm quality, so it is essential to test the semen before insemination. Traditional semen quality analysis methods, such as inspection and analysis under a light microscope, are convenient, quick and cheap. Traditional semen quality testing parameters can only give a rough idea of ​​...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 高宁赵云翔江威朱琳彭兴张从林刘沁沅郑伟
Owner 广西贵港秀博基因科技股份有限公司
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