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Silver hexosamine dyeing kit, silver hexosamine dyeing method, and application of protective protein agent in silver hexosamine dyeing field

A technology for protecting proteins and staining reagents, which is applied in the field of hexamine silver staining, which can solve the problems of affecting observation and diagnosis under the microscope, being unable to remove, and dirty tissue sections, so as to achieve clear visibility of sectioned tissues, improve the staining effect, and increase the staining efficiency. Effect

Pending Publication Date: 2019-09-03
河南赛诺特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the growth of time, obvious silver mirror reaction began to appear around the glassware, that is, the glassware was coated with a layer of metallic silver, and the stained slides were also coated with a layer of silver, and the tissue sections looked special. Dirty, cannot be removed at all by subsequent wiping treatments
After staining, the stained tissue parts on the section will be observed under the microscope, and there will be non-specific staining, which will affect the observation and diagnosis under the microscope

Method used

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  • Silver hexosamine dyeing kit, silver hexosamine dyeing method, and application of protective protein agent in silver hexosamine dyeing field
  • Silver hexosamine dyeing kit, silver hexosamine dyeing method, and application of protective protein agent in silver hexosamine dyeing field
  • Silver hexosamine dyeing kit, silver hexosamine dyeing method, and application of protective protein agent in silver hexosamine dyeing field

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] The tissue to be stained in the hexamine silver staining method used in this embodiment is kidney tissue; use the staining kit in Example 1 of the hexamine silver staining kit for staining, which includes the following steps:

[0092] (1) Periodic acid aqueous solution is oxidized to the tissue for 15 minutes;

[0093] (2) Rinse with purified water, soak for 5min×2 times;

[0094] (3) Put the freshly prepared hexaamine silver staining solution into a 64°C water bath and heat; the hexaamine silver staining solution is prepared from liquid A and liquid B in the kit at a volume ratio of 49:1;

[0095] After the temperature of the hexamine silver staining solution was constant, put the tissue sections into the staining solution for 15 minutes;

[0096] (4) Rinse with purified water, soak for 5min×2 times;

[0097] (5) Sodium thiosulfate solution was treated for 3 minutes;

[0098] (6) Counterstaining with hematoxylin for 3 minutes;

[0099] (7) Rinse with purified water, ...

Embodiment 2

[0102] In the hexamine silver staining method in this example, the dyeing kit in Example 2 of the hexamine silver staining kit is used for staining, and the staining steps are the same as in Example 1 of the hexamine silver staining method.

Embodiment 3

[0104] In the hexamine silver staining method in this example, the dyeing kit in Example 3 of the hexamine silver staining kit is used for staining, and the staining steps are the same as in Example 1 of the hexamine silver staining method.

[0105] The hexamine silver staining solution prepared in the embodiment 1-3 of the hexamine silver staining method is stained for 15min and the section tissue obtained by color and staining is as follows: image 3 Shown, wherein, A is the embodiment 1 of hexamine silver staining method; B is the embodiment 2 of hexamine silver staining method; C is the embodiment 3 of hexamine silver staining method. It can be seen from the figure that adding BSA will significantly improve the staining effect and staining intensity. During the staining process, the staining vat and the staining sheet will not appear dirty with the traditional staining solution; especially the tissue staining on the sheet with different amounts of BSA added Intensity will ...

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PUM

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Abstract

The invention relates to a silver hexosamine dyeing kit, a silver hexosamine dyeing method, and application of protective protein agent in a silver hexosamine dyeing field, and belongs to the technical field of silver hexosamine dyeing. An experiment discovers that BSA (Bull Serum Albumin), PHA (Phytohemagglutinin), escherichia coli protein, gelatin and milk powder can improve silver hexosamine dyeing efficiency and improve a dyeing effect, wherein the dyeing effect of the BSA is best; the protective protein agents can be firstly combined with silver ions in mixed dyeing solution, and therefore, a situation that residual silver ions are subjected to nonspecific binding attachment on a glass sheet is avoided; meanwhile, mucopolysaccharide on tissues are oxidized into aldehyde by periodic acid, so that an aldehyde group can be strongly combined with the silver ions, and finally, specific tinting is formed. Therefore, the protective protein agent can be used in the silver hexosamine dyeing field and is used for preparing the silver hexosamine dyeing kit.

Description

technical field [0001] The invention relates to a hexamine silver staining kit, a staining method thereof, and an application of a protective protein agent in the field of hexamine silver staining, and belongs to the technical field of hexamine silver staining. Background technique [0002] Hexamine silver staining is a common special stain in routine pathological techniques, and it is used as a diagnostic basis for showing changes in fungi and glomerular basement membranes in pathological diagnosis. The hexaamine silver method is sensitive to staining of various fungi, and can be easily found even in a small amount of fungi. It is of great value in the identification of Aspergillus and Trichozyme. It can be combined with immunofluorescence, electron microscopy and other techniques to comprehensively and correctly diagnose and study kidney disease. It can make up for the methodological defect that HE staining method is difficult to observe the change of renal capillary basem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/31
CPCG01N1/31
Inventor 梁永波刘玲玲陈玲李莉刘文弟齐华张娟丽于婷
Owner 河南赛诺特生物技术有限公司