Cyclodepsipeptide compound in mangrove endophytic fungi as well as preparation method and application of cyclodepsipeptide compound

A technology of compound and cyclic depsipeptide, which is applied in the field of cyclic depsipeptide compound and its preparation in mangrove endophytic fungi, can solve side effects and other problems, and achieve the effects of short growth cycle, easy accumulation, and easy expansion of cultivation

Active Publication Date: 2019-09-10
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the current cancer treatment drugs are cytotoxic drugs, which can inhibit the proliferation of ...

Method used

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  • Cyclodepsipeptide compound in mangrove endophytic fungi as well as preparation method and application of cyclodepsipeptide compound
  • Cyclodepsipeptide compound in mangrove endophytic fungi as well as preparation method and application of cyclodepsipeptide compound
  • Cyclodepsipeptide compound in mangrove endophytic fungi as well as preparation method and application of cyclodepsipeptide compound

Examples

Experimental program
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Effect test

Embodiment 1

[0055] tcctTCCGTAggggaACCTGCGGAAGGATCATTAACCTTTCAAAAAACAGGGTGCGTCGCGGCCCCCCTCCGGGGGCGGTTTGGGGTCCTCCCCTTCACGCGCGCACGACTGCATCCTTACTTTACGAGCACCTTCTGTTCTCCCTCGGCGGGGCAACCTGCCGTTGGAACCGAATAAACCCTTTTTTGCATCCAGCATTACCCGTTCCGAAACAAACAATCGTTACAACTTTCAACAATGGATCTCTTGGCTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATCTACACCCTCAAGCTCTGCTTGGTGTTGGGCGTCTGTCCCGCCTCAGCGCGCGGACTCGCCCCAAATCCATTGGCAGCGGTCCTTGCCTCCTCTCGCGCAGCACATTGCGCTTCTCGAGGGGCTCCGGCCCGCGTCCAACAAGAAAACATTACCGTCTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAAtaaacgGAGGAaac(SEQ ID NO.:1),鉴定该菌株属于Pseudopithomyces属真菌,为已知菌株。 The preparation of embodiment 1 formula (I) cyclic peptide compound

[0056] Prepare formula (I) cyclic peptide compound according to the following steps:

[0057] 1. Fermentation of the fungus Pseudopithomyces sp.

[0058] Take out the slant preservation tube of Pseudopithomyces sp., put it at room temperature for 0.5h, connect Pse...

Embodiment 2

[0062] The preparation of embodiment 2 formula (I) cyclic peptide compound

[0063] Prepare formula (I) cyclic peptide compound according to the following steps:

[0064] 1. Fermentation by the fungus Pseudopithomyces sp.

[0065] Take out the slant preservation tube of Pseudopithomyces sp., put it at room temperature for 0.5h, connect Pseudopithomyces sp. to the PDA plate in the ultra-clean bench for activation, place it in an incubator at 26°C for 4 days, and then transfer it to 6 bottles of In a sterile 500mL Erlenmeyer flask containing 250mL of PYG liquid medium, place it on a shaker at 26°C and culture at 100rpm for 5 days as a seed culture solution. The preparation of PYG liquid culture is based on 500mL Erlenmeyer flasks, each bottle contains 250mL of PYG, an appropriate amount of seed solution is added to each bottle, a total of 200L is inoculated, and the culture is placed at 28°C for 28 days to complete the fermentation.

[0066] 2. Extraction and separation of sec...

Embodiment 3

[0068] The preparation of embodiment 3 formula (I) cyclic peptide compound

[0069] Prepare formula (I) cyclic peptide compound according to the following steps:

[0070] 1. Fermentation by the fungus Pseudopithomyces sp.

[0071] Take out the slant preservation tube of Pseudopithomyces sp., place it at room temperature for 0.5h, connect Pseudopithomyces sp. to a PDA plate in an ultra-clean bench for activation, place it in an incubator at 30°C for 2 days, and then transfer it to 6 bottles of In a sterile 500mL Erlenmeyer flask containing 250mL of PYG liquid medium, place it on a shaker at 28°C and 90rpm for 3 days as a seed culture solution. The preparation of PYG liquid culture is based on 500mL Erlenmeyer flasks, each bottle contains 250mL of PYG, an appropriate amount of seed solution is added to each bottle, a total of 200L is inoculated, and the culture is placed at 28°C for 32 days to complete the fermentation.

[0072] 2. Extraction and separation of secondary metabo...

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Abstract

The invention discloses a cyclodepsipeptide compound which has a structural formula (I) shown in the specification. The compound can be prepared by separating and purifying fermentation products of anendophytic fungus Pseudopithomyces sp. of leaves of a mangrove plant, namely sonneratia caseolaris. In vitro tests show that the cyclodepsipeptide compound has an obvious cell proliferation inhibition effect on HELA (human cervical carcinoma cell) strains, A549 human lung cancer cell strains and SHSY5Y human bone marrow neuroblastoma cell strains and can be used for preparing antitumor drugs.

Description

technical field [0001] The invention belongs to the field of medicines, and more specifically relates to a cyclic depsipeptide compound of mangrove endophytic fungi and its preparation method and application. Background technique [0002] Malignant tumor is one of the serious diseases that endanger human health and life at present. According to the latest national cancer statistics, an average of more than 10,000 people are diagnosed with cancer every day, and 7 people are diagnosed with cancer every minute. The standard rate is 186.53 / 100,000, and the cumulative incidence rate of 0-74 years old is 21.58%. Most of the current cancer treatment drugs are cytotoxic drugs, which can inhibit the proliferation of normal cells in the body while inhibiting the proliferation of tumor cells, resulting in certain side effects. Therefore, it is of great significance to study new anti-tumor drugs with high targeting and few side effects. [0003] Most of the synthetic chemical anti-tu...

Claims

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Application Information

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IPC IPC(8): C07D273/08A61P35/00A61K31/395C12P17/14C12R1/645
CPCC07D273/08A61P35/00C12P17/14
Inventor 温露陈钢邬桂梅
Owner GUANGDONG PHARMA UNIV
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