A novel oncolytic virus and its preparation method and application

An oncolytic virus, a new type of technology, applied in the field of biomedicine, can solve problems such as weakening inhibitory activity, and achieve the effects of improving killing function, good targeting and anti-tumor effect, and good application prospects
CN110218707BActive Publication Date: 2021-10-22SHANGHAI PUBLIC HEALTH CLINICAL CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI PUBLIC HEALTH CLINICAL CENT
Publication Date
2021-10-22

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Abstract

The invention discloses a novel oncolytic virus and its preparation method and application. The oncolytic virus genome contains gene coding sequences of anti-human CTLA4 antibody and human IL-21, and can express anti-human CTLA4 antibody and human IL-21 21. Combining the tumor-suppressing effect of immunotherapy with the oncolytic effect of viral therapy, an oncolytic virus capable of highly expressing the αCTLA4-Fc(ALIE)-IL21 gene was prepared, and the oncolytic virus exerted an oncolytic effect to lyse the tumor At the same time, it can kill regulatory T cells (Regulatory T Cells, Treg) in the tumor microenvironment by expressing a large amount of αCTLA4‑Fc (ALIE), or block its inhibitory effect, thereby up-regulating the immune response of the tumor microenvironment and improving the anti-tumor effect At the same time, the oncolytic virus expresses a large amount of IL21, further activates the killing function of T cells and NK cells in the tumor microenvironment, and exerts multiple anti-tumor effects. Compared with simple gene therapy or virus therapy, it has enhanced its ability to kill malignant tumors.
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Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and specifically relates to a novel oncolytic virus comprising a modified or engineered highly lethal anti-human CTLA4 antibody and a human IL-21 gene, a preparation method of the oncolytic virus, and an anti-tumor effect application. Background technique

[0002] Due to the limited therapeutic effect and severe side effects of standard cancer treatment regimens in the treatment of advanced tumors, tumor-targeting oncolytic viruses have attracted great attention in recent decades. Oncolytic viruses benefit from their own characteristics of selectively infecting and lysing tumor cells locally in tumors, and are increasingly becoming the first choice for anti-tumor. The tumor selectivity of oncolytic viruses can be either their own tumor tropism or the result of genetic modification. Oncolytic viruses can act on multiple cellular pathways to reduce tumor resistance and induce different forms ...

Claims

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