Method for identifying resistance of tomato leaf mold by using in-vitro leaves

A technology of tomato leaf mold and detached leaves, applied in the field of tomato leaf mold, can solve the problems of difficult preservation of rare resources, high cost, long detection cycle, etc., and achieve low detection cost, good for preservation, and small space occupation Effect

Pending Publication Date: 2019-09-13
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The invention solves the problems existing in the current identification method of tomato leaf mold resistance, such as long detection period, high cost, or being easily affected by the environment, direct damage to identification materials, and difficult preservation of rare resources, etc., using tomato detached leaves to inoculate leaf mold pathogenic bacteria, evaluate the degree of disease incidence under precise experimental conditions, and test the resistance of different tomato resources to leaf mold

Method used

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  • Method for identifying resistance of tomato leaf mold by using in-vitro leaves
  • Method for identifying resistance of tomato leaf mold by using in-vitro leaves
  • Method for identifying resistance of tomato leaf mold by using in-vitro leaves

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Embodiment 1

[0057] (1) Planting of tomato seedlings: The tomato variety "Nippon Mikado" and "Beiying" were raised in the greenhouse. Before the seedlings were raised, the utensils and 50-hole seedling trays were washed and sterilized with 5% sodium hypochlorite. The nutrient soil for seedlings was vermiculite , perlite, peat (1:1:3) mixed compound soil, sow double seeds after high temperature sterilization, keep 1 seedling in each hole, 2 trays for each material, 50 plants in each tray. Take it when the seedlings are about 30 days old (5-6 true leaves).

[0058] (2) Treatment of tomato leaves: the leaves to be tested are the 2nd to 4th leaves (about 5-7cm in size), with the same maturity, rinsed with detergent water for 2 minutes, rinsed with tap water, and then rinsed with sterile water Rinse twice, dry naturally, and set aside.

[0059] (3) Leaf mold culture: Take leaf mold leaves from typical diseased plants in the field, and separate and purify them on PDA medium. And place it in a ...

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Abstract

The present invention discloses a method for identifying resistance of tomato leaf mold by using in-vitro leaves. The method comprises tomato in-vitro leaf collection, fulvia fulva (cooker) ciferri culture and spore suspension preparation, in-vitro leaf inoculation, disease investigation, etc. The method mainly utilizes the cultivated fulvia fulva (cooker) ciferri to inoculate on the in-vitro leaves of the tomato material to identify the resistance of the tomato material to pathogenic micro-organisms. The method utilizes a piece of the in-vitro leaf indoors to identify the disease resistance of the material, and is simple in operation, good in controllability and high in efficiency, and a simple and effective method for inoculation identification. The method can scientifically, efficientlyand accurately identify the resistance of different tomato materials to the leaf mold.

Description

technical field [0001] The invention relates to tomato leaf mold, more specifically to a method for identifying tomato resistance by using tomato leaf mold pathogenic bacteria to inoculate tomato detached leaves. Background technique [0002] Tomato leaf mold occurs all over the world, and mainly damages the leaves. Leaf infection mostly occurs on the middle and lower leaves, and then expands upwards. At first, yellow chlorotic spots with unclear edges appear on the front of the leaves, and the back of the leaves is densely gray and white at first. Velvet-like mold layer, later turning purple gray or dark gray to black or yellowish brown, polygonal or irregular brown spots appear on the leaves in severe cases, and then the brown spots gradually expand, with yellow halos around, multiple lesions merge and connect The leaves are curled and withered, and the plants dry up early, which seriously reduces the yield and quality of tomatoes. The causative agent of tomato leaf mold ...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/645
CPCC12Q1/18G01N2333/37
Inventor 吉立柱王倩郏艳红
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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